Su Xiu-rong

Main nutritional contents of 30 Dalian coastal microalgae species

This paper reports results of study on the contents of proteins, amino acids, polysaccharose and uronic acids in 30 species of macroalgae from Shicao, Heishijiao, Shimiao, and Xiaofujiazhuang in the vicinity of Dalian City, N.E.China. The results showed that the protein contents of the 30 algae from highest (112.55 μ g/ml) to the lowest (0.24 μg/ml) was in the descending order ofDictyopteris ndalata, Gelidium vagum, Gymnogongrus japonican, Ectocarpus confervoides, Tinocladia crassa, Sargassum thunberii. In general, the protein content in red algae was higher than that in brown algae. The content of free amino acids showed no significent differences from 7.44 μg/ml4.96 μg/ml in all these algae, in the descending order ofGymnogongrus japonican, Sargassum confusum, Undoria pinnatifida, Laminaria japonica andEctocarpus confervoides. The content of polysaccharose varied from 168.2 μ/ml-22.15 μg/ml in the descending order ofSymphocladia latiuscula, Scytosiphon lomentarius, Desmarestia viridis., Tinocladia crassa, Gracilaria asiatica andPorphyra yezoensis. The content of uronic acids is from 196.24μg/ml-20.77 μg/ml in the descending order ofUlva lactuca, Symphyoclaldia latiuscula, Scytosiphon lomentarius, Ceramimum kodoi, Gracilaria vemucosa andPorphyra yezoensis. The fatty acids in 30 species of algae belong to Rhodophyta, Chlorophyta and Phaeophyta. Most phaeophytes have many (4–12) types of fatty acids.

泥蚶( Tegillarca granosa )重组铁蛋白富集锰铁后的磁学性质解析

采用聚丙烯酰胺凝胶同工酶电泳技术对韩国釜山、山东荣成、浙江奉化和乐清、福建福鼎、广东汕头和湛江7个地理群体的泥蚶进行同工酶分析，结果发现在所检测的MDH、ADH、ATPase、LDH、POD、IDH、SOD、SDH、ME、SCD和EST等11种同工酶中，除ATPase外其他10种在各地理群体之间存在着或大或小的差异，特别是汕头和湛江两地理群体与其他5个地理群体之间的这种差异尤其显著，两组间的平均酶距达0．4209，而汕头和湛江群体间的差异较小，两者酶距为0．0909；釜山、荣成、奉化、乐清、福鼎5个群体间的平均酶距也只有0．0191。这说明上述7个地理群体泥蚶已明显分化为两大类群，汕头和湛江群体为一类群，其他5个群体为一类群，两大类群的分界线在东海和南海的分界处。还对不同泥蚶群体间的同工酶分子标记的建立、不同群体间的遗传变异水平及泥蚶群体间分化的可能机制等问题进行了探讨，以期为泥蚶资源的进一步开发和利用提供科学资料。

泥蚶( Tegillarca granosa )重组铁蛋白富集重金属离子的特性及化学传感器的研究

采用不连续垂直聚丙烯酰胺凝胶电泳法,对泥蚶(Tegillarca granosa Linnaeus)19个发育阶段的ADH、LDH、EST、IDH、ALP、MDH、GDH、POD、ATPase和ME等10种同工酶的基因表达进行检测分析.结果表明,泥蚶在个体发育过程中随着机体分化和生理活动的改变,同工酶基因的表达呈现出显著的时序变化.在10种酶中,LDH和ATPase两种酶的所有酶带及EST、MDH和ME等的部分酶带在各个发育阶段持续表达,其他酶带只出现在发育阶段的某一或某些阶段,并且表现出了与泥蚶营养方式、代谢形式等生理活动的关联性.在泥蚶的个体发育阶段过程中,IDH、 MDH、GDH、ME、ALP等5种酶在32细胞期表达的酶带数剧增,因此32细胞期可能是合子基因表达启动的时间; ADH、EST、LDH、MDH、GDH、ME等6种酶在泥蚶的附着变态期酶带大幅减少或产生新酶带,可能与泥蚶附着期运动方式、代谢强度及生活环境的改变有关.