Subir Bera

Phytochemical and genetic analyses of ancient cannabis from Central Asia

The Yanghai Tombs near Turpan, Xinjiang-Uighur Autonomous Region, China have recently been excavated to reveal the 2700-year-old grave of a Caucasoid shaman whose accoutrements included a large cache of cannabis, superbly preserved by climatic and burial conditions. A multidisciplinary international team demonstrated through botanical examination, phytochemical investigation, and genetic deoxyribonucleic acid analysis by polymerase chain reaction that this material contained tetrahydrocannabinol, the psychoactive component of cannabis, its oxidative degradation product, cannabinol, other metabolites, and its synthetic enzyme, tetrahydrocannabinolic acid synthase, as well as a novel genetic variant with two single nucleotide polymorphisms. The cannabis was presumably employed by this culture as a medicinal or psychoactive agent, or an aid to divination. To our knowledge, these investigations provide the oldest documentation of cannabis as a pharmacologically active agent, and contribute to the medical and archaeological record of this pre-Silk Road culture.

Quantifying the rise of the Himalaya orogen and implications for the South Asian monsoon

We reconstruct the rise of a segment of the southern flank of the Himalaya-Tibet orogen, to the south of the Lhasa terrane, using a paleoaltimeter based on paleoenthalpy encoded in fossil leaves from two new assemblages in southern Tibet (Liuqu and Qiabulin) and four previously known floras from the Himalaya foreland basin. U-Pb dating of zircons constrains the Liuqu flora to the latest Paleocene (ca. 56 Ma) and the Qiabulin flora to the earliest Miocene (21- 19 Ma). The proto-Himalaya grew slowly against a high (similar to 4 km) proto-Tibetan Plateau from similar to 1 km in the late Paleocene to similar to 2.3 km at the beginning of the Miocene, and achieved at least similar to 5.5 km by ca. 15 Ma. Contrasting precipitation patterns between the Himalaya-Tibet edifice and the Himalaya foreland basin for the past similar to 56 m.y. show progressive drying across southern Tibet, seemingly linked to the uplift of the Himalaya orogen.

Herbivore Damage to Ferns Caused by a Chrysomelid Beetle from Lower Gangetic Plains of West Bengal, India

ABSTRACT This paper records the occurrence of a polyphagous beetle, Schenklingia bhaumiki (Coleoptera: Chrysomelidae), feeding on ten fern species growing in the Lower Gangetic Plains of West Bengal, India viz., Christella dentata, Ampelopteris prolifera, Cyclosorus sp., Pteris vittata, Nephrolepis cordifolia, N. exaltata, Adiantum philippense, Drynaria propinqua, Pyrrosia adnascens and Phymatosorus scolopendria for the first time. The adult beetles are leaf surface scraper and skeletonize the lamina. The larvae are leaf miners and produce linear–blotch mines between the epidermal layers with continuous spiral black frass. Young leaves of all ten species of ferns are significantly less damaged than mature ones indicating that both the adults and the larvae attack leaves of all ages. Herbivore damage of the beetle infested ferns ranged from 1.94% to 25.47% and 2.68% to 54.86% for scraping feeding and mining feeding respectively. Among the host ferns, the members of Thelypteridaceae viz., Christella (Scraping feeding 25.47%; mining feeding 54.86%), Ampelopteris (Scraping feeding 24.10%; mining feeding 53.60%) and Cyclosorus (Scraping feeding 16.06%, mining feeding 27.12%) suffered maximum herbivore damage. Interspecific variation of plant size and biogeographic range of the fern species are not related to herbivore damage. Insects may perhaps attack fewer ferns than angiosperms, but there is no evidence that ferns are generally less damaged than angiosperms.

On some fabaceous fruits from the Siwalik sediments (Middle Miocene-Lower Pleistocene) of Eastern Himalaya

Four legume fruits (Fabaceae) from the Siwalik sediments (middle Miocene to lower Pleistocene) of Darjeeling and Arunachal Pradesh of Eastern Himalaya are described here. One fossil fruit, Dalbergia prelatifolia sp. nov., is recovered from the lower part of the Siwalik succession (Gish Clay Formation of Sevok Group; middle to upper Miocene) of Darjeeling foothills. Mastertia neoassamica sp. nov. and Acacia miocatechuoides sp. nov. are collected from the lower part of the Siwalik succession (Dafla Formation; middle Miocene to upper Miocene), while Pongamiakathgodamensis Prasad is recovered from the upper part of the Siwalik succession (Kimin Formation; upper Pliocenelower Pleistocene) of Arunachal sub Himalaya. Their presence indicates a warm and humid tropical environment in the region during the Siwalik sedimentation.

Pollen and Phytoliths from Fired Ancient Potsherds as Potential Indicators for Deciphering Past Vegetation and Climate in Turpan, Xinjiang, NW China

It is demonstrated that palynomorphs can occur in fired ancient potsherds when the firing temperature was under 350°C. Pollen and phytoliths recovered from incompletely fired and fully fired potsherds (ca. 2700 yrs BP) from the Yanghai Tombs, Turpan, Xinjiang, NW China can be used as potential indicators for reconstructing past vegetation and corresponding climate in the area. The results show a higher rate of recovery of pollen and phytoliths from incompletely fired potsherds than from fully fired ones. Charred phytoliths recovered from both fully fired and incompletely fired potsherds prove that degree and condition of firing result in a permanent change in phytolith color. The palynological data, together with previous data of macrobotanical remains from the Yanghai Tombs, suggest that temperate vegetation and arid climatic conditions dominated in the area ca. 2700 yrs BP.

Evidence for diversification of Calophyllum L. (Calophyllaceae) in the Neogene Siwalik forests of eastern Himalaya

Here, we report fossil leaves, woods, and pollen grains comparable to Calophyllum L. (mainly to Calophyllum inophyllum L. and Calophyllum polyanthum Wall. ex Choisy) of Calophyllaceae from the upper (Kimin Formation, late Pliocene-early Pleistocene), middle (Subansiri Formation, Pliocene) Siwalik sediments of the Arunachal sub-Himalaya, and lower (Gish Clay Formation of Sevok Group; middle to late Miocene) Siwalik sediments of the Darjeeling foothills, eastern Himalaya. Their presence indicates a warm and humid tropical environment in the region during the period of Siwalik sedimentation. Considering all records of Calophyllum, it is suggested that Calophyllum was a frequent forest element throughout the period of Siwalik sedimentation during the Neogene (Miocene time). At present, C. polyanthum grows in the eastern Himalaya, but C. inophyllum is totally absent from north-eastern regions suggesting differential adaptability of these taxa to changing ecoclimatic conditions. Distinct climate change in the area, possibly related to the Himalayan Orogeny during Miocene–Pleistocene times, might have caused the disappearance of C. inophyllum from the entire eastern Himalaya and north-east Indian plains and a move to littoral/coastal and swampy forests of India and other adjoining south-east Asian regions, Polynesia, and the east coast of Africa. The past global distribution of Calophyllum is also discussed, and it is suggested that India may have been its primary centre of origin. This is the first time Cenozoic fossil leaves Calophyllum siwalikum Khan, R.A.Spicer & Bera, sp. nov. comparable to C. inophyllum are described using the both macro- and micromorphological characters.

First megafossil evidence of Cyatheaceous tree fern from the Indian Cenozoic

A part of the compressed tree fern axis with leaf and adventitious root scars in unusual arrangement from Plio–Pleistocene sediments of Arunachal Pradesh, India is described as Cyathea siwalika sp. nov. This record suggests that Cyathea was an important component of tropical evergreen forest in the area during the Plio–Pleistocene and this group subsequently declined in importance within the local vegetation.

Study on the selection of some local pollen plants in West Bengal, India as pollen brood feed in Apis cerana Fabr. Larvae by the worker bees, through meconia analysis and community ordination method of recovered pollen taxa

Pollen analytical data of meconia from worker bee larvae of Apis cerana Fabr. collected in the summer (June, 2008) from West Bengal, India (22.35°N, 88.44°E) are presented. Following the community ordination method, the pollen analytical data were used to determine the preference of pollen feeding by the worker bees to the larvae destined to be worker of Apis cerana, which could be recognized as a new protocol in order to ascertain the probable larval food spectrum. Meconium samples were collected from 9 different sites having box hives. Microscopic analysis yields pollen grains of 10 different taxa viz., Carica papaya L. (Caricaceae), Borassus flabellifer L., Cocos nucifera L. (Arecaceae), Psidium guajava L., Eucalyptus sp., Syzygium sp., (Myrtaceae), Punica granatum L. (Punicaceae), Litchi chinensis Sonn. (Sapindaceae), Ziziphus sp. (Rhamnaceae) and Dalbergia sissoo Roxb. ex DC. (Papilionaceae). The community ordination method reveals that the most preferred pollen taxa are of Carica papaya, Borassus flabellifer and Psidium guajava although they were not recovered in significant frequency. The present result may be an useful information for the apiarists, especially during site selection for hive installation.

Soral Crypsis: Protective Mimicry of a Coccid on an Indian Fern

Herbivory with crypsis is not well documented in ferns. The present record of cryptic coloration of coccid Saissetia filicum Boisduval (Homoptera: Coccidae) to the sori of a fern species Asplenium nidus L. (Aspleniaceae) is unique. Predatory beetles (Jauravia sp., Coleoptera: Coccinellidae) that feed on the coccids, are suggested to be selective pressure for the development of the present homopteran soral crypsis. A higher rate of effective predation is noticed in the vegetative leaves than the fertile leaves. Aggressive ants were found harvesting honeydew secretions from the coccids and defending the trophobionts as well as the host fern from their natural enemies. In addition, a possible three-way mutualistic relationship among the coccids, its host fern and the tending ant is suggested. Differential numbers of coccids on vegetative and fertile leaves is correlated with their phenol content and degree of predation by beetles. Such coloration mimicry by the coccids may enable them to obtain the necessary blend of sorus of the host fern needed to evade beetle detection and attack.

First fossil evidence of Connaraceae R. Br. from Indian Cenozoic and its phytogeographical significance

Fossil leaflet impression described here as a new species Rourea miocaudata sp. nov., showing close resemblance with the modern leaflets of Rourea caudata Planch. (Connaraceae R. Br.), has been recorded from the lower part of the Siwalik sediments (Dafla Formation, middle–upper Miocene) exposed at the road-cutting section of Pinjoli area in West Kameng district, Arunachal Pradesh. The important morphological characters of the fossil are its narrow elliptic leaflet, cuneate base, long caudate apex, entire margin, eucamptodromous to brochidodromous secondary veins, presence of intersecondary veins, percurrent and reticulate tertiary veins and orthogonally reticulate quaternary veins. This is the first authentic record of the occurrence of leaflet comparable to R. caudata of Connaraceae from the Cenozoic sediments of India and abroad. At present R. caudata does not grow in India and is restricted only in southeast Asia especially in China and Myanmar. This taxon probably migrated to these southeast Asian regions after lower Siwalik sedimentation (middle–upper Miocene) due to climatic change caused by post-Miocene orogenic movement of the Himalaya. The recovery of this species and other earlier-described evergreen taxa from the same formation, suggests the existence of a tropical, warm and humid climatic conditions during the depositional period.