Sufia Rahman

Micropropagation of Morus laevigata Wall. from mature trees.

Multiple shoots were obtained from nodal explants of 10-year-old tree of Morus laevigata on Murashige and Skoogs medium supplemented with different concentrations (0.5–5.0 mg.l−1) of benzyladenine (BA). Nodal segments taken from in vitro proliferated shoots gave further multiple shoots when cultured on the same basal medium containing 2.5 mg.l−1 BA. Repeated subculture resulted in rapid shoot multiplication at the average rate of 6-fold per subculture. In vitro raised shoots rooted on MS medium containing 0.1 mg. l−1 each of 3-indolebutyric acid (ISA) and α-naphthaleneacetic acid (NAA). The regenerated plantlets were successfully established in soil under field conditions after a few days of indoor acclimatization.

Production of plantlets from Aegle marmelos nucellar callus

Techniques have been developed for the regeneration of Aegle marmelos from nucellar explants. Slow-growing calli were induced from nucellar explants excised from 90–120 d-old developing fruits. The medium consisted of Murashige and Skoog formulation containing 40 g/l sucrose, 400 mg/l casein hydrolysate, 5 mg/l 1-naphthaleneacetic acid and 1 mg/l kinetin. The basal medium with high concentration (1–5 mg/l) of N6-benzyladenine (BA) and low concentration (0.1 mg/l) of NAA was suitable for regeneration of shoots from 3-month-old calli. Addition of 1 mg/l gibberellic acid (GA3) favoured shoot growth. Callus-derived shoots produced roots and developed into plantlets when transferred to half-strength MS medium supplemented with 0.5 mg/l indole-3-butyric acid (IBA) and 0.5 mg/l NAA. Approximately 5 months were required for the full regenerative process.

Effects of media composition and culture conditions on in vitro rooting of rose

Abstract Basal medium strength, auxins, sucrose, agar, pH, photoperiod and culture room temperature substantially affected the development of roots from proliferated rose (cultivar ‘Tajmahal’) shoots. Half strength Murashige and Skoog (MS) medium fortified with 0.1 mg l−1 naphthaleneacetic acid (NAA) and 0.5 mg l−1 indolyl-3-acetic acid (IAA), sucrose level of 40 g l−1, agar concentration at 6 g l−1, a 16 h photoperiod and 28°C culture room temperature were required for optimal in vitro rooting of the excised shoots. Gradual acclimatization was essential for subsequent establishment of plantlets in natural condition.

High efficiency plant regeneration from petiole explants of Carica papaya L. through organogenesis

Callus cultures were obrained from petiole explants of Carica papaya on MS medium containing 0.5–10.5 μM α-naphthaleneacetic acid (NAA) in combination with 0.5–5 μM benzyladenine (BA). Hard-green calli were transferred to MS medium containing 100 mgl−1 casein hydrolysate (CH) with specific BA-NAA formulation, where they developed adventitious buds within 2 weeks of culture. Maximum number of adventitious buds were obtained in 2 μM BA and 0.1 μM NAA. Shoot regeneration occurred from these adventitious buds by the end of the 4th week. Regenerated shoots were elongated in hormone-free medium and rooted in half-strength MS fortified with 3 UM NAA and 0.5 μM gibberellic acid (GA3). The regenerants were transferred to soil after acclimatization.

A Class of Poisson Mixtured Distributions

Abstract In this paper, a class of mixtured distributions is defined which is known as “Poisson mixture of distributions”. Some characteristics of these distributions are then provided.

TCTAP A-182 Immediate In-Hospital Complications of Percutaneous Transvenous Mitral Commissurotomy in Patients with Mitral Stenosis

Rheumatic fever and rheumatic heart disease continue to be the major health problem in all developing countries including Bangladesh. Rheumatic mitral stenosis is a very common problem in our population having an incidence of 54 percent among rheumatic heart disease with a female preponderance of 2: