Suhaib A. Bandh

Fungal endophytes as prolific source of phytochemicals and other bioactive natural products: A review

Endophytic fungi are those that live internally in apparently healthy and asymptomatic hosts. Endophytic fungi appear to be ubiquitous; indeed, no study has yet shown the existence of a plant species without endophytes. High species diversity is another characteristic of endophytic mycobiota which is depicted by the fact that it is quite common for endophyte surveys to find assemblages consisting of more than 30 fungal species per host plant species. Medicinal plants had been used to isolate and characterize directly the bioactive metabolites. However, the discovery of fungal endophytes inside these plants with capacity to produce the same compounds shifted the focus of new drug sources from plants to fungi. Bioactive natural products from endophytic fungi, isolated from different plant species, are attracting considerable attention from natural product chemists and biologists alike which is clearly depicted by the steady increase of publications devoted to this topic during the recent years. This review will highlight the chemical potential of endophytic fungi with focus on the detection of pharmaceutically valuable plant constituents as products of fungal biosynthesis. In addition, it will cover newly discovered endophytic fungi and also new bioactive metabolites reported in recent years from fungal endophytes. It summarizes the up-to-date and comprehensive information on bioactive compounds from endophytic fungi by having done a thorough survey of literature.

In vitro and in vivo anthelmintic activity of Euphorbia helioscopia L.

The anthelmintic potentials of the aqueous and methanol extracts of Euphorbia helioscopia were investigated. In folklore medicine, E. helioscopia (Euphorbiaceae) is used in the treatment of various gastrointestinal ailments and diseases. A worm motility inhibition (WMI) assay and egg hatch assay (EHA) were used for in vitro assessment, and a faecal egg count reduction (FECR) assay was used for an in vivo study. The in vitro study revealed anthelmintic effects of crude methanolic extracts of E. helioscopia on live Haemonchus contortus worms as evident from their paralysis and/or death at 8h after exposure, different concentrations (12.5 mg ml(-1), 25 mg ml(-1) and 50 mg ml(-1)) of aqueous and methanolic extracts were used against H. contortus which exhibited dose-dependent anthelmintic effects on H. contortus. Aqueous and methanolic extracts of aerial parts (stem, leaves and flowers) of E. helioscopia were found to have very low percent inhibitory egg hatching as compared to levamisole. It is concluded that the entire plant of E. helioscopia possesses significant anthelmintic activity and could be a potential alternative for treating cases of helminth infections in ruminants.

Phytochemical screening, antimicrobial and antioxidant efficacy of different extracts of Rumex dentatus L. – A locally used medicinal herb of Kashmir Himalaya

Objective To elucidate the antimicrobial and antioxidant activities of Rumex dentatus L. (R. dentatus) along with its phytochemical analysis.

Identification of some Penicillium species by traditional approach of morphological observation and culture

Maximum percentage of fungal colonies (27.7%) was observed at site PKB followed by TBN (21.13%), DLG and GB (13.15%) each, NL (8.45%), BHN (7.04%), HB (5.63%) and BD (3.76%).

Aquatic bacterial diversity: Magnitude, dynamics, and controlling factors

The primary aspiration in the microbial observatory is to advance the understanding of freshwater bacterioplankton, whose diversity and population dynamics are currently the least understood off all freshwater planktonic organisms. Through identification and characterization of bacterial populations in a suite of fresh water bodies, we are able to gain significant new insight into the ecological niches of bacteria in diverse freshwater ecosystems. Given the facts that lakes and other inland freshwaters play a more critical role in the global carbon budget and that lakes have been described as early indicators of both regional and global environmental change, the role of microbes in these processes is of renewed interest. In this review, general overview will be given highlighting the characteristic features of bacterial species thriving in different water bodies. In the following sections, different cultural approaches vis a vis the controlling factors of bacterial diversity have been elaborated. In the concluding sections, the prospects of aquatic microbial diversity are well mentioned.

Isolation, Identification and Distribution of Culturable Bacteria in Manasbal Lake, Kashmir Himalaya

Bacteria comprise an important part of the aquatic microbial flora and perform myriad of ecological roles in aquatic ecosystems. Yet the bacteriological studies on aquatic ecosystems are scarce, especially in the Himalayan context. The main objective of present study was to assess the bacteriological composition of Manasbal lake, the deepest freshwater lake of Kashmir Himalayas, in relation to anthropogenic pressures. The authors measured abundance of the culturable bacteria in lake waters from March to December 2011 using culture based approaches. A total of 1391 different colonies of the identified bacterial strains were obtained from the lake waters with significant site specific variability. The data indicated that the colony number was highest for site II (surrounded by residential hamlets) followed by site I (an irrigational stream) and III (Central site) and lowest for site IV (Outlet). The strains were identified using the gram’s staining, motility test and biochemical tests. Data revealed a highest percentage occurrence of 14.67 % for Proteus II and lowest of 0.21 % for Shigella I. This study indicated the need for development of a feasible system with regular assessment of microbial population for ascertaining the proper microbial load in order to effectively combat the multiple dangers of anthropogenic pollution of the lake.

Enhanced production of α-amylase by Penicillium chrysogenum in liquid culture by modifying the process parameters.

In this paper, we have assessed the role of changing physicochemical parameters and substrate types on the production of α-amylase enzyme from Penicillium chrysogenum, with a view to determining the optimal conditions required for its maximum production. The findings of this research revealed that, at pH 6 using linseed oil cake as substratum, α-amylase enzyme production was maximum (550.0 U/mL), when the fungi was incubated for 6 days at 30 °C in 0.1 M acetate buffer. Further, reasonably good production of the α-amylase enzyme was also observed at pH 9 with all the experimented carbon sources as substrates. Moreover, statistical analysis, using analysis of variance (ANOVA) carried out to study the impact of different studied parameters on the α-amylase enzyme production revealed that incubation period of 6-18 days is highly significant (p = 0.01) factor in amylotic activity of the P. chrysogenum. Under the researched out optimal conditions, P. chrysogenum is an economically viable option for the industrial and biotechnological production of α-amylase enzyme.

Municipal solid waste generation rates and its management at Yusmarg forest ecosystem, a tourist resort in Kashmir

The present study was carried out at Yusmarg, a forest ecosystem and tourist resort, in the Kashmir valley during 2012 with the objectives of determining the municipal solid waste (MSW) generation rates per capita and on a daily basis, and assessing the existing MSW system. It was estimated that daily generation of MSW at Yusmarg by tourists, as well as residents, was 107.74 kg; on average, the MSW generated at each site was about 36.48 kg/day. The per capita generation of MSW was highest (0.97 kg/person/day) at site 1 followed by 0.288 kg/person/day at site 2 and 0.201 kg/person/day at site 3, with an average per capita MSW generation rate of 0.484 kg/person/day. Manual segregation of the collected wastes showed that it comprised some recyclable, combustible, compostable and inert materials. Among the different waste categories, 56% of waste was recyclable materials, 29% was compostable wastes, 9% was combustible wastes and 6% was inert materials. The present study infers that MSW management in Yusmarg was inappropriate, and infrastructure, skilled manpower and a proper scientific disposal mechanism is lacking in the area. In order to conserve the forest wealth of the area there is a great need to focus on the solid waste problem of the tourist resort.

Opportunistic fungi in lake water and fungal infections in associated human population in Dal Lake, Kashmir.

Natural habitats of opportunistic fungal pathogens are outside of the host; therefore, it is critically important to understand their ecology and routes of transmission. In this study, we investigated the presence of human pathogenic opportunistic fungi in lake water and incidence of fungal infections in associated population in Kashmir, India. Six hundred forty water samples were taken on seasonal basis from a wide network of sampling stations of the lake for an extended period of two years for screening their occurrence. The samples were inoculated onto rose bengal agar, malt extract agar, potato dextrose agar and other specified culture media supplemented with Chloramphenicol and Streptomycin followed by incubation at 37 °C. All the samples were positive for fungi, which were later identified by sequencing the rDNA internal transcribed spacer region aided by classical morphological culture techniques and physiological profiling. The whole process led to the isolation of sixteen species of opportunistic fungal pathogens belonging to genus Aspergillus, Candida, Penicillium, Cryptococcus, Fusarium, Rhizopus and Mucor in decreasing order of prevalence. Furthermore, 20% population (n = 384) of Dal inhabitants was examined for possible fungal infections and it was observed that only 8.07% individuals were positive for fungal infections with 4.68% skin infection cases, 2.34% onychomycosis cases and 1.04% candidiasis cases. Scrapings from onychomycosis and candidiasis patients showed the presence of Aversicolor and Calbicans respectively, resembling exactly the strains isolated from the lake water. However, the skin infection was because of a dermatophyte not isolated for the lake water. Higher prevalence of infection (6.77%) was seen in people using lake water followed by a positive prevalence of 1.30% using tap water. The results of present study suggest that the lake inhabitants are at a greater risk of getting life threatening fungal diseases which may lead to various morbidities.

Dynamics of bacterial class Bacilli in the deepest valley lake of Kashmir-the Manasbal Lake

In recognition of the importance of bacteria as ecological indicators of the aquatic systems a comprehensive and systematic analysis was carried out on Manasbal Lake, the deepest spring fed valley lake of Kashmir. The main objective envisaged was to analyze bacterial community composition (BCC) and for this purpose systematic and regular sampling of waters from ten different sampling stations, predetermined in the Lake according to differences in degree of human interference and also as zones of special ecological interests were selected. The isolated species were identified according to Bergeys Manual specification by examining their micro and macro morphological characteristics and biochemical characteristics on different culture media. Further confirmation was done by sequencing the 16s rRNA gene by using universal bacterial primers 27F and 1429R. From all the sampling stations the class Bacilli showed a maximum relative abundance with a contribution of 16 bacterial species. The whole process resulted in the identification of Bacillus aerius, Bacillus altitudinis, Bacillus anthracis, Bacillus cereus, Bacillus ginsengisoli, Bacillus pumilus, Bacillus safensis, Bacillus stratosphericus, Bacillus subtilis, Bacillus tequilensis, Bacillus thermocopriae, Bacillus thuringiensis, Brevibacillus agri strain, Lysinibacillus boronitolerans, Lysinibacillus pakistanensis and Lysinibacillus sphaericus.