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Featured researches published by Suk-Weon Kim.


BMC Genomics | 2012

Small RNA and transcriptome deep sequencing proffers insight into floral gene regulation in Rosa cultivars

Jungeun Kim; June Hyun Park; Chan Ju Lim; Jae Yun Lim; Jee-Youn Ryu; Bong-Woo Lee; Jae-Pil Choi; Woong Kim; Ha Yeon Lee; Yourim Choi; Donghyun Kim; Cheol-Goo Hur; Suk-Weon Kim; Yoo-Sun Noh; Chanseok Shin; Suk-Yoon Kwon

BackgroundRoses (Rosa sp.), which belong to the family Rosaceae, are the most economically important ornamental plants—making up 30% of the floriculture market. However, given high demand for roses, rose breeding programs are limited in molecular resources which can greatly enhance and speed breeding efforts. A better understanding of important genes that contribute to important floral development and desired phenotypes will lead to improved rose cultivars. For this study, we analyzed rose miRNAs and the rose flower transcriptome in order to generate a database to expound upon current knowledge regarding regulation of important floral characteristics. A rose genetic database will enable comprehensive analysis of gene expression and regulation via miRNA among different Rosa cultivars.ResultsWe produced more than 0.5 million reads from expressed sequences, totalling more than 110 million bp. From these, we generated 35,657, 31,434, 34,725, and 39,722 flower unigenes from Rosa hybrid: ‘Vital’, ‘Maroussia’, and ‘Sympathy’ and Rosa rugosa Thunb. , respectively. The unigenes were assigned functional annotations, domains, metabolic pathways, Gene Ontology (GO) terms, Plant Ontology (PO) terms, and MIPS Functional Catalogue (FunCat) terms. Rose flower transcripts were compared with genes from whole genome sequences of Rosaceae members (apple, strawberry, and peach) and grape. We also produced approximately 40 million small RNA reads from flower tissue for Rosa, representing 267 unique miRNA tags. Among identified miRNAs, 25 of them were novel and 242 of them were conserved miRNAs. Statistical analyses of miRNA profiles revealed both shared and species-specific miRNAs, which presumably effect flower development and phenotypes.ConclusionsIn this study, we constructed a Rose miRNA and transcriptome database, and we analyzed the miRNAs and transcriptome generated from the flower tissues of four Rosa cultivars. The database provides a comprehensive genetic resource which can be used to better understand rose flower development and to identify candidate genes for important phenotypes.


Plant Cell Tissue and Organ Culture | 2003

High frequency somatic embryogenesis and plant regeneration in petiole and leaf explant cultures and petiole-derived embryogenic cell suspension cultures of Hylomecon vernalis

Suk-Weon Kim; Dong Su In; T.J. Kim; Jang Ryol Liu

Culture conditions are described for high frequency somatic embryogenesis and plant regeneration in petiole and leaf explant cultures and petiole-derived embryogenic cell suspension cultures of Hylomecon vernalis Max. Petiole explants formed embryogenic calluses at a frequency of 53% when cultured on B5 medium supplemented with 13.6 μM 2,4-dichlorophenoxyacetic acid (2,4-D) alone. Leaf explants formed embryogenic calluses at a frequency of 21% when cultured at a combination of 4.52 μM 2,4-D and 2.22 μM 6-benzyladenine. Cell suspension cultures were established with petiole-derived embryogenic calluses using liquid B5 medium with 4.52 μM 2,4-D. Upon plating onto B5 basal medium, cell suspension cultures produced numerous somatic embryos, which then developed into plantlets. Regenerated plantlets were transplanted to potting soil and grown to maturity in a greenhouse.


Plant Molecular Biology Reporter | 2009

Production of Fibrinolytic Enzyme in Plastid-Transformed Tobacco Plants

Suk-Min Ko; Byung-Ho Yoo; Jong-Min Lim; Kwang-Hoon Oh; Jae-Il Liu; Suk-Weon Kim; Jang-Ryol Liu; Kwan-Sam Choi; E. S. Yoon

The earthworm fibrinolytic enzyme, which belongs to a group of serine proteases with strong fibrinolytic activity, has been used as an oral drug for prevention and treatment of thrombosis in East Asia. Fibrizyme is a fibrinolytic enzyme isolated from the earthworm Eisenia andrei. Here we report genetic engineering of tobacco plastids with stable integration of the fibrizyme gene into the tobacco chloroplast genome. A plastid transformation vector was constructed by introducing various regulatory elements into fibrizyme cDNA. This vector was delivered by particle bombardment into tobacco leaf explants and plastid-transformed plants were subsequently regenerated into whole plants through several rounds of selection. We confirmed stable integration of the fibrizyme gene into the tobacco plastid genome by PCR and Southern blot analyses. Northern and Western blot analyses revealed that mRNA and protein of recombinant fibrizyme were highly expressed in transformed tobacco plants.


Journal of Plant Biotechnology | 2007

Genetic Discrimination of Catharanthus roseus Cultivars by Multivariate Analysis of Fourier Transform Infrared Spectroscopy Data

Suk-Weon Kim; Soohwa Cho; Hoeil Chung; Jang-R. Liu

To determine whether pattern recognition based on metabolite fingerprinting for whole cell extracts of higher plants is applied to discriminate plants genetically, leaf samples of eight cultivars of Catharanthus roseus were subjected to Fourier transform infrared spectroscopy (FT-IR). FT-IR fingerprint region data were analyzed by principal component analysis (PCA). Major peaks as biomarkers were identified as the most significant contributors to distinguish samples by using genetic programming. A hierarchical dendrogram based on the results from PCA separated the eight cultivars into two major groups in the same manner as the dendrograms based on genetic fingerprinting methods such as RAPD and AFLP. A slight difference between the dendrograms was found only in branching pattern within each subgroup. Therefore, we conclude that the hierarchical dendrogram based on PCA of the FT-IR data represents the most probable chemotaxonomical relationship between cultivars, which is in general agreement with the genetic relationship determined by conventional DNA fingerprinting methods.


Phytochemistry | 1996

Production of petroselinic acid from cell suspension cultures of Coriandrum sativum

Suk-Weon Kim; Mi-Kyung Park; Kyung-Sook Bae; Moon-Soo Rhee; Jang-Ryol Liu

Abstract The fatty acid pattern and petroselinic acid content in calli, somatic embryos and cell suspension cultures of Coriander sativum are described. The petroselinic acid content was 0.15–0.23 mg g −1 fresh wt of calli, somatic embryo and cell suspension cultures and varied little with callus origin and culture conditions. The ratio of unsaturated: saturated fatty acid was ca 4: 1 in calli. However, the unsaturated fatty acid content was greater in somatic embryos and cell suspension cultures.


Journal of Plant Biotechnology | 2007

Plant Regeneration from Floral Stem Cultures of Nymphoides indica (L.) O. Kuntze. via Somatic Embryogenesis

Myung-Jin Oh; Sung-Ran Min; Jang-Ryol Liu; Suk-Weon Kim

Plant regeneration system from floral stem of Mymphoides indica via somatic embryogenesis was established. After four weeks of culture onto 1/2MS medium containing 2,4-D, pale-yellow globular structures and calluses were formed on the cut surface of floral stem explants. Upon transfer to 1/2MS basal medium, pale-yellow globular structures were developed into somatic embryos and normal plantlets. These results indicated that pale-yellow globular structures and calluses from floral stem were globular embryos and embryogenic calluses, respectively. The frequency of embryogenic callus formation from floral stem was reached to nearly 100% when floral stem was cultured onto 1/2Ms medium supplemented with low concentration of 2,4-D (0.1 to 0.3 mg/L). However, the higher concentration of 2,4-D resulted in decrease of the frequency of embryogenic callus formation. In this study, low concentration of 2,4-D had a stimulative role in embryogenic callus formation, whereas BA showed inhibitory role in callus formation. In comparison to floral stem, leaf explants showed low frequency of embryogenic callus formation. The highest frequency of embryogenic callus formation from leaf explants was 9.5% when leaf explants were cultured onto 1/2MS medium supplemented with 0.3 mg/L of 2,4-D. The plant regeneration system of Nymphoides indica established in this study, might be applied to mass proliferation, conservation of genetic resources and genetic transformation for molecular breeding.


Journal of Plant Biotechnology | 2008

In vitro seed germination and callus formation on flower bud of Korean mistletoe ( Viscum album L. var. cololatum [Kom.] Ohwi)

Suk-Weon Kim; Suk-Min Ko; Jang-R. Liu

겨우살이의 캘러스 형성 및 종자 발아 와 haustorium 발달에 요구되는 환경요인 및 생장조절제의 영향을 조사하였다. 아울러 종자의 인위부착을 통한 식물체 재생 체계를 확립하였다. 겨우살이 조직관찰 결과 종자에는 1개 또는 2개의 막대모양의 접합자배가 존재하며 종자의 외부에 위치한 유근 부위에 주로 세포분열이 왕성한 세포층이 존재하며 반대로 자엽은 종자 내부에 위치하며 상대적으로 분열능을 가진 세포수가 감소하였다. 겨우살이 종자 발아 와 haustorium 발달에 요구되는 여러 요인 중에서 광의 요구도가 절대적이었다. 본 실험에 조사된 다른 배양 환경요인 및 생장조절제들은 광의 효과를 대체하지 못하였으며 ethylene의 경우 발아촉진 효과가 3배 이상 증가함을 알 수 있었다. 여러 조직 중에서 오로지 겨우살이 화아로부터 캘러스 형성이 가능하였으며 캘러스 형성빈도는


Journal of Plant Biotechnology | 2011

Myo-inositol increases the plating efficiency of protoplast derived from cotyledon of cabbage (Brassica oleracea var. capitata)

Eun-Yee Jie; Suk-Weon Kim; Hye-Rim Jang; Dong-Su In; Jang-Ryol Liu

0.1\;mgl^{-1}


Journal of Plant Biotechnology | 2010

Current status on plant molecular farming via chloroplast transformation

Sung-Ran Min; Won-Joong Jeong; Suk-Weon Kim; Jeong-Hee Lee; Hwa-Jee Chung; Jang-R. Liu

IAA가 첨가된 B5 배지에 배양 시 27.3%로 가장 높았다. 본 연구에서 확립된 겨우살이 캘러스 배양 및 종자 인위 부착을 통한 식물체 재생체계는 겨우살이의 유용물질 생산 연구 및 대량증식 연구분야에 활용이 가능할 것으로 기대된다. 【Effects of growth regulators and culture conditions on seed germination, haustorium development, and callus formation of Korean mistletoe (Viscum album var. coloratum (Kom.) Ohwi) were described. Histological examination showed that seed of V. album contained one or two zygotic embryos with rod shape, and actively dividing cells were mainly distributed in radicle region rather than cotyledon of zygotic embryo. The most significant factor for seed germination and haustorium development of V. album was the requirement of the light. Various growth regulators examined in this study failed to substitute the effect of the light on seed germination. The frequency of callus formation was highest at 27.3% when flower buds were cultured onto B5 medium containing


Journal of Plant Biotechnology | 2007

Establishment of a Simple and Rapid Method for Quantitative Determination of -Aminobutyric Acid Using 1 H NMR and Production of γ-Aminobutyric Acid in Cell Suspension Cultures of Coriandrum sativum L.

Suk-Weon Kim; Jang-R. Liu

0.1\;mgl^{-1}

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Jang-Ryol Liu

Korea Institute of Science and Technology

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Jong-Du Lee

Chungbuk National University

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Suk-Min Ko

Jeju National University

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Hwa-Jee Chung

Korea Research Institute of Bioscience and Biotechnology

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Jong Hyun Kim

Korea Research Institute of Bioscience and Biotechnology

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Thanh-Tam Ho

Chungbuk National University

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Won-Joong Jeong

Korea Research Institute of Bioscience and Biotechnology

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