Süleyman Akbulut

Description of Bursaphelenchus anamurius sp. n. (Nematoda: Parasitaphelenchidae) from Pinus brutia in Turkey.

Bursaphelenchus anamurius sp. n. isolated from wilted Turkish pine (Pinus brutia Ten.) wood is described. The new species shares several characters with the hofmanni-group sensu Braasch (2001). It is characterised by a body length of 633 (540-749) μm and 744 (638-867) μm for males and females, respectively, stout body (a = 29 in male and 27 in female), stylet 14 μm long in both males and females, lateral field with three lines, length of postuterine branch one-third to one-half of the vulva to anus distance, conoid female tail (c′ = 3.4) with a finely rounded or pointed terminus, very delicate and relatively straight spicules 10 (9-11) μm long with compact capitulum, broad, not distinctly offset condylus and strong rostrum with a more or less rounded tip lacking a cucullus, shovel-shaped bursa and the presence of seven caudal papillae. Bursaphelenchus anamurius sp. n. differs from other species of the hofmanni-group mainly by having smaller, unusually shaped spicules, shorter condylus and no cucullus. The new species can be differentiated from other Bursaphelenchus species morphologically and by means of ITS-RFLP patterns, particularly by digestion of the PCR product with RsaI, HaeIII, MspI, HinfI and AluI.

Comparison of the reproductive potential of Monochamus galloprovincialis on two pine species under laboratory conditions

Monochamus galloprovincialis Olivier (Coleoptera: Cerambycidae) is one of the vectors of the pinewood nematode Bursaphelenchus xylophilus (Steiner and Buhrer) Nickle (Nematoda: Parasitaphelenchidae), the causal agent of pine wilt in susceptible pine trees. It is therefore important to study both the biological and ecological characteristics of the beetle. In this study reproductive potentials of female beetles reared on two pine species were investigated under laboratory conditions and compared using fertility tables constructed for beetles reared on each tree species. Beetle population parameters were compared between tree species. The intrinsic rate of increase and associated population parameters of beetles reared on Pinus sylvestris L. were consistently higher than those reared on P. nigra Arnold. This result suggests that P. sylvestris has a significantly greater positive effect on the reproductive potential of M. galloprovincialis populations than does P. nigra.

Bursaphelenchus Fuchs, 1937 (Nematoda: Parasitaphelenchidae) species associated with Pinus species in northern Turkey

SummaryA survey for Bursaphelenchus nematodes, associated with different conifer trees, was conducted in several forest areas in the northern regions of Turkey. Only pine trees (Pinus nigra, P. pinaster and P. sylvestris) yielded Bursaphelenchus specimens. Nematodes were identified using several morphological diagnostic characters of the genus (male spicule structure, number of lateral incisures, number and distribution of the male papillae, presence of female vulval flap), and confirmed by using RFLP analysis of the internal transcriber spacer (ITS) regions of ribosomal DNA. Three different species were identified from several sampled areas, namely B. mucronatus, B. pinophilus and B. sexdentati, representing a first report of the last two species for Turkey. The association of B. pinophilus with black pine (P. nigra) is herein reported for the first time.

Pine Wilt Disease: A Threat to Pine Forests in Turkey?

The pinewood nematode (PWN), is the causal agent of pine wilt disease, and constitute one of the most important pathogens of conifer forests. In 1999, this nematode was found and identified for the first time in Portugal and in Europe. The detection of this quarantine pest in Portugal has prompted the need to know more about the distribution of Bursaphelenchus spp. in coniferous trees in Europe in order to describe the geographic range of the species and to act quickly in case of the nematode’s unwanted introduction into other European regions. Pine forest has a wide distribution in Turkey, which increases the number of susceptible host trees for the PWN. Because of these reasons, some regions of Turkey were surveyed for the presence of the nematode. Three different species of Bursaphelenchus were found, however, B. xylophilus was not detected. The detection of B. mucronatus, very similar to B. xylophilus biologically and morphologically, is very important. The presence of this species indicates that B. xylophilus could spread easily into the conifer forests of Turkey. Biological characteristics of M. galloprovincialis were compared with M. carolinensis, the North American insect vector, and some of them were found to be similar.

Comparison of pathogenic potential of Bursaphelenchus species on conifer seedlings between greenhouse and outdoor conditions

Bursaphelenchus xylophilus is a serious threat to the European conifer forests. In Turkey, four Bursaphelenchus species (B. mucronatus, B. sexdentati, B. anamurius and B. vallesianus) were isolated from wilted pine trees. To determine the effects of Bursaphelenchus species on the wilting of pine trees, a pathogenicity study was conducted comparing greenhouse and outdoor conditions. Three-year-old seedlings of Pinus nigra, P. sylvestris and P. pinaster were used for inoculation tests. Approximately 1000 (±100) nematodes of all life stages in 0.5 ml distilled water were inoculated into each seedling. The first wilting symptoms were observed in the fifth week. Mortalities occurred between the 5th and 13th weeks of the study. B. mucronatus was the most pathogenic nematode species. The most susceptible seedling species was P. pinaster, and P. nigra was the most resistant species. The mean number of wilted seedlings differed significantly among nematode species and seedling species but not between greenhouse and outdoor conditions.

Morphological and molecular characterisation of Bursaphelenchus andrassyi sp. n. (Nematoda: Aphelenchoididae) from Romania and Turkey

Bursaphelenchus andrassyi sp. n., found in conifer wood samples from Romania and Turkey, is characterised morphologically and genetically. Bursaphelenchus andrassyi sp. n. clearly belongs to the sexdentati group, having a terminal bursa, four lateral lines, a very small female ‘vulval flap’, strongly arcuate spicules and the typical position of caudal papillae of males. It is morphologically most similar to B. vallesianus and B. sexdentati. It can be differentiated from B. vallesianus by the usually subcylindrical female tail with rounded or wedge-shaped terminus vs conical female tail with a more or less rounded terminus, slightly different shape of spicules (low square condylus, lacking a distinct cucullus, pointed rostrum) and from B. sexdentati by lacking a distinct post-vulval constriction, shorter stylet and shorter spicules. The species status is supported by ITS-RFLP patterns and sequencing results of both partial 18S and 28S rDNA regions.

Effect of log seasonality on reproductive potential ofMonochamus galloprovincialis reared in scots pine logs under laboratory conditions

Insect reproduction may be affected by a number of factors including seasonal changes in larval or adult nutrition. The effect of season on the reproductive potential ofMonochamus galloprovincialis (Olivier) females reared inPinus sylvestris L. (Scots pine) logs was investigated by constructing fertility tables for each log that differed only in the season they were cut. Population parameters were compared among three seasonal cohorts. The intrinsic rate of increase and most of the associated population parameters of beetles that emerged from logs cut during spring were higher than for beetles emerged from summer and autumn logs. Slight differences were found between summer and autumn cohorts. We suggest that seasonal differences in the nutritional quality of logs caused differences inM. galloprovincialis survival and reproductive potential.

A STUDY ON TROUT BREEDING IN DUZCE PROVINCE

In this study, trout breeding facilities of Duzce Province, a suitable place for trout breeding, were evaluated. As a result of this evaluation, current status, capacities, and problems of trout breeding facilities were determined. Some suggestions were provided to solve these problems and increase the capacity of trout breeding facilities. Keywords: Duzce, Trout Breeding.