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Gene | 1986

Direct expression of urogastrone gene in Escherichia coli

Kishimoto Fumitaka; Gomi Hideyuki; Kanaoka Masaharu; Nakatani Tomoyuki; Ito Akira; Katoh Tomoko; Agui Hideo; Sumida Seizo; Ogino Shigeo

Abstract Human epidermal growth factor (urogastrone; UG) is a 53-amino acid polypeptide hormone. A 192-bp DNA fragment containing the coding sequence for methionyl UG (Met-UG) and the ribosome-binding site (RBS) was chemically synthesized and placed downstream from the promoter for the Escherichia coli outer-membrane lipoprotein gene (Ipp) on a plasmid. E. coli cells harboring the plasmid directed the synthesis of Met-UG at 10 2 -10 2 molecules per cell. Next, the coding sequence for Met-UG was inserted in a runaway-replication plasmid and expressed under the control of the lpp promoter and the RBS derived from bacteriophage Mu cII gene. Upon heat induction, the cells harboring the recombinant plasmid synthesized 10 5 molecules of Met-UG per cell.


Archive | 1978

N{40 -{8 4-(substituted phenethyloxy)phenyl{9 -N-methyl-N-methoxyurea

Takemoto Ichiki; Yoshida Ryo; Sumida Seizo; Kamoshita Katsuzo


Archive | 1979

3-SUBSTITUIERTE ANILINDERIVATE, VERFAHREN ZU IHRER HERSTELLUNG UND IHRE VERWENDUNG

Kossuge Yoshiaki; Yoshida Ryo; Sumida Seizo


Archive | 1979

N'-PHENYL-N-METHYLHARNSTOFF-DERIVATE

Takemoto Ichiki; Sumida Seizo; Yoshida Ryo; Kamoshita Katsuzo


Archive | 1979

N'-PHENYL-N-METHYLHARNSTOFFDERIVATE, VERFAHREN ZU IHRER HERSTELLUNG UND IHRE VERWENDUNG ZUR UNKRAUTBEKAEMPFUNG

Takemoto Ichiki; Sumida Seizo; Yoshida Ryo; Kamoshita Katsuzo


Archive | 1979

PROCEDE DE PRODUCTION DE DERIVES D'ANILINE SUBSTITUEE EN POSITION 3, NOUVEAUX PRODUITS AINSI OBTENUS ET LEUR UTILISATION COMME HERBICIDES ET COMME FONGICIDES

Kosuge Yoshiaki; Yoshida Ryo; Sumida Seizo


Archive | 1978

N'-(4-PHENETHYLOXY SUBSTITUE)PHENYL) - N - METHYL - N - METHOXYUREES ET LEUR UTILISATION COMME HERBICIDES

Takemoto Ichiki; Yoshida Ryo; Sumida Seizo; Kamoshita Katsuzo


Archive | 1977

N-(4-BENZYLOXYPHENYL)-N'-METHYL-N'- METHOXYHARNSTOFF, VERFAHREN ZU SEINER HERSTELLUNG UND SEINE VERWENDUNG ALS HERBIZID INSBESONDERE IN SOJABOHNENKULTUREN

Yoshida Ryo; Sumida Seizo; Itooka Eiyoshi; Noguchi Hiroshi; Kamoshita Katsuzo; Toyonaka Osaka


Archive | 1981

2-Alkoxy pyridazinoquinazolinone derivatives as herbicides

Asao Shuichiro; Nakayama Yoshinori; Yoshida Ryo; Sumida Seizo; Hashimoto Shunichi


Archive | 1980

3,5-DIOXO-1,2,4-TRIAZINE UND DEREN SALZE, VERFAHREN ZU IHRER HERSTELLUNG UND VERWENDUNG

Katoh Haruhiko; Oshio Hiromichi; Hashimoto Shunichi; Sanemitsu Yuzuru; Mizutani Masato; Sumida Seizo

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