Sun Lian-na

Chemical constituent analysis of EtOAc fraction of Tithonia diversifolia(HEMSL.) A. Gray

Objective To isolate and identify the chemical constituents of the EtOAc fraction of Tithonia diversifolia.Methods The dried aerial parts of Tithonia diversifolia were extracted with 80% EtOH,and the chemical constituents of the ethyl acetate portion from the extract were isolated by column chromatography with silica gel,Sephadex LH-20 and MCI.The isolated compounds were identified by NMR and MS analysis.Results Ten compounds were isolated from EtOAc extract,and identified as:vanillin(1),ergosterol(2),ergosterol peroxide(3),3-methoxy-4-hydroxy-trans-cinnamaldehyde(4),p-hydroxybenzaldehyde(5),7-methylesculetin(6),5-hydyoxymethyl furfural(7),nepetin(8),docosanoic acid 2,3-dihydroxypropyl ester(9) and 13-hydroxyeupatorochromene(10).Conclusion Vanillin,ergosterol,ergosterol peroxide,3-methoxy-4-hydroxy-trans-cinnamaldehyde,p-hydroxybenzaldehyde,7-methylesculetin,5-hydyoxymethyl furfural,docosanoic acid 2,3-dihydroxypropyl ester,and 13-hydroxyeupatorochromene have been isolated from Tithonia diversifolia for the first time.

Studies on chemical constituents of Chaenomeles speciosa (Sweet) Nakai (II).

Objective:To investigate the chemical constituents of Chaenomeles speciosa.Methods:The chemical constituents of Chaenomeles speciosa was analyzed by various chromatographic methods.The structures of the compounds were identified based on physicochemical properties and spectral data.Results:Nine compounds were obtained from the ethyl acetate fraction of C.speciosa and were identified as methyl chlorogenate (Ⅰ),5-O-cafeoyl quinic acid butyl ester (Ⅱ),quinic acid butyl ester (Ⅲ),5-hydroxymethyl-furan-2-carbaldehyde (Ⅳ),triacontanoic acid (Ⅴ),(-)-epicatichin (Ⅵ),7,8-dihydroxy-coumanrin (Ⅶ),protocatechuic acid ethyl ester (Ⅷ),and protocatechuic acid (Ⅸ).Conclusion:Compounds Ⅲ-Ⅷ have been obtained from this genus for the first time.