Sun Sheng-gang

Study on the relationship between plasma homocysteine and acute cerebral vascular disease.

The levels of plasma homocysteine were determined by using high-performance liquid chromatographic method. It was found that plasma homocysteine levels were significantly higher in the patients with stroke than that in the controls. There was no correlation between plasma homocysteine levels and hypertension, smoking, concentrations of blood glucose or hypertriglyceridesemia. It was suggested that hyperhomocysteinemia may be an independent risk factor for acute cerebral vascular disease.SummaryThe levels of plasma homocysteine were determined by using high-performance liquid chromatographic method. It was found that plasma homocysteine levels were significantly higher in the patients with stroke than that in the controls. There was no correlation between plasma homocysteine levels and hypertension, smoking, concentrations of blood glucose or hypertriglyceridesemia. It was suggested that hyperhomocysteinemia may be an independent risk factor for acute cerebral vascular disease.

Experimental Study on the Neuronal Toxical Effect of Levodopa and the Inhibition of Ginkgo Biloba Extract on Parkinson Disease in Rats

Objective: To observe neuronal toxical effect of Levodopa and investigate whether using Levodopa together with EGb is an ideal, workable method to treat Parkinson disease. Methods: In this study, the rat models of Parkinson disease (PD) were made by injecting stereotaxically 6-OHDA to right side of the mesencephic ventral tegmental area (VTA) and substantia nigra pars compacta (SNc). We used rotational behaviour, TUNEL, immunocytochemical, Nissl’s body staining methods to observe the difference between Levodopa (50mg/kg/d×3d, ×5d, ×7d, L-dopa group) and the combination use of Levodopa and EGb (100 mg/kg/d, E-D group). Results: The numbers of apoptosis and rotation, bFGF protein expression in the L-dopa group surpassed those in the E-D group (P<0.05). The number of Nissl cells in the L-dopa group was fewer than the E-D group. Conclusion: Levodopa has neurological toxical effect. EGb may decrease the toxicity of levodopa. The combination use of Ginkgo biloba extract (EGb) and Levodopa is a workable method to treat Parkinson disease and is better than using Levodopa alone.

Experimental Study on Parkinson Disease Model of Rats

Objective: To observe the characteristics of praxiology of Parkinson disease (PD) rats. Methods: At 1d, 7d, 14d after PD rats’ model were successful; we observed the markers of rotational behavior of Parkinson disease (PD) rats. For example: start time, continuous time, maximum turning, etc. Results: From 1d to 14d, PD rats, start time of the rotational behavior elongated gradually, continuous time shortened by degrees, maximum rotational speed and the number of the rotational behavior were invariable. Conclusions: Improved method of PD is scientific, reliable, and simple. Substantial nigral pathology of PD rats makes the foundation of the rotational behavior. The injury of substantial nigral can be reflected by maximum rotational speed and the number of the rotational behavior of PD rats.

Experimental Study on the Connection of Nigral Apoptosis of Normal Rats and Rats of Parkinson Disease with Different Doses of Levodopa

Objective: To evaluate the neuronal toxicical effects induced by levodopa and explore the method in which PD can be treated reasonably with levodopa. Method: The rat models of Parkinson disease (PD) were made by injecting stereotaxically 6-OHDA to right side of the mesencephic ventral tegmental area (VTA) and substantia nigra pars compacta (SNc). The PD rats were treated intraperitoneally with different dosages (10 mg/kg/d, 50 mg/kg/d, 100 mg/kg/d) and different durations (1 d, 3 d, 5 d, 7 d) of levodopa. At the same time, we observed the injury of substantia nigral by the way of treating PD rats with levodopa for 7 days and then stopped for 7 days. In this study, we used TUNEL to detect the injury of substantia nigra by different doses and durations of levodopa. Results: The number of substantia nigral apoptosis was increased with the changes of doses and durations of levodopa. Conclusions: If we use levodopa intermittently or as little as possible, the toxical action of levodopa may be reduced.

Study on relationship between anticardiolipin antibody and cerebrovascular diseases

SummarySerum anticardiolipin antibody (ACA) was measured in 91 patients with cerebral infarction (CI), 42 patients with cerebral hemorrhage (CH) and 30 healthy controls. The results showed that the ACA in CI and CH patients was significantly higher than in controls and IgG-ACA was the most important iso-type. Stroke in ACA positive group tended to be recurrent and of multi-focuses. Positive rate of IgG-ACA reached its peak within the first week after stroke on-set. The results suggested that ACA was an independent risk factor in CI and CH and might be valuable in stroke prediction.