Sun ZhongWu

A preliminary research of genetic linkage map construction and QTL analysis for growth-related traits in yellow catfish (Pelteobagrus fulvidraco).

Genetic linkage maps of yellow catfish (Pelteobagrus fulvidraco) were constructed using SSR,SRAP and TRAP markers and a pseudo-testcross mapping strategy. A segregating population including 100 progenies was sampled within the first filial generation obtained by artificial fertilization between a male parent and a female parent from wild and breeding populations respectively. Totally,13 SSR,89 SRAP and 26 TRAP markers were used to construct a genetic map. Female map included 16 linkage groups,covering a total of 585.5 cM in length. Male map included 15 linkage groups,covering a total of 752.3 cM in length. Combined map included 5 linkage groups,covering a total of 231.3 cM in length. This map was used to scan the QTL related to growth traits of yellow catfish. One head length QTLs were identified in female map located in LG7,LOD value is 3.2 and accounted for 13 % of phenotypic variation. A body width and a body length QTLs were identified in male map both located in LG1,LOD value is 2.4 and 2.1 respectively,and accounted for 12% and 11% of phenotypic variation respectively. Three QTLs could be considered to apply in marker-assisted selection for growth-related traits breeding of yellow catfish.

The actions, detection and synthesis of platelet-activating factor (PAF) in mammalian pregnancy

Platelet-activating factor (PAF) exhibits a variety of biological activities and it be thought to involved in various pathophysiological process. In this paper, some studies were summarized about those roles of PAF in a variety productive processes of female of mammalian that include fertilization, implantation and parturition, and that was involved in the concentration, synthesis, degradation and some assay methods of PAF. The relationship between PAF and early pregnancy factor (EPF) was reviewed.

Research progress about nature of early Pregnancy factor

Platelet-activating factor (PAF) exhibits a variety of biological activities and it be thought to involved in various pathophysiological process. In this paper, some studies were summarized about those roles of PAF in a variety productive processes of female of mammalian that include fertilization, implantation and parturition, and that was involved in the concentration, degradation and some assay methods of PAF. The relationship between PAF and early pregnancy factor(EPF was reviewed.

Study on Isozyme Electrophoresis of Martes zibellina L.

Four isozymes, such as Malate dehydrogenase (MDH), Alchol dehydrogenase (ADH), Peroxidae (POD) and Esterase (EST) in six tissues (heart, liver, kidney, muscle, eye, gonad) ofMartes zibellina L., were analyzed by means of vertical polyacrylamide gel electrophoresis (PAGE). The results indicated that the zymograms of these four isozymes in different tissues were different from each other, i.e. there existed apparent tissue-specificitity in these isozymes inMartes zibellina L.. Characteristic enzyme band was found both in POD zymogram and in EST zymogram. Moreover, the characteristic enzyme band in POD isozyme would be of some value to sexual identification ofMartes zibellina L.