Sung Hee Choi
Kyungsung University
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Publication
Featured researches published by Sung Hee Choi.
Biotechnology Letters | 2009
Sung Hee Choi; Hee Sook Kim; Eun Yeol Lee
The epoxide hydrolase (EH) of a marine fish, Mugil cephalus, was engineered to improve the catalytic activity based on comparative homology modeling. The 3-D crystal structure of the EH from Aspergillus niger was used as a template. A triple point mutant, F193Y for spatial orientation of the nucleophile (D199), W200L for removing electron density overlap between W200 and Y348, and E378D for good charge relay in the active site, was developed. The initial hydrolysis rate, the reaction time to reach 98 %ee, and yield were enhanced up to 35-fold, 26-fold and 32%, respectively, by homology modeling-inspired site-directed mutagenesis of M. cephalus EH.
Biotechnology Letters | 2010
Sung Hee Choi; Hee Sook Kim; In Su Lee; Eun Yeol Lee
A triple-point mutated fish microsomal epoxide hydrolase (mEH) gene from Mugil cephalus was expressed in Escherichia coli in the presence of various chaperones to prevent protein aggregations. The enantioselective hydrolytic activity was more than doubled by co-expressing the EH mutant gene with pGro7 plasmid. The highly active EH mutant with a his-tag was immobilized onto magnetic silica assembled with NiO nanoparticles. The immobilized mEH mutant was re-used more than 10 times with less than 10% activity loss. (S)-Styrene oxide with 98% enantiopurity was repeatedly obtained with over 50% of the theoretical yield by the magnetically separable high-performance mEH mutant.
Bioprocess and Biosystems Engineering | 2011
Jung Won Shin; Sung Hee Choi; Dong Eun Kim; Hee Sook Kim; Jae-Hwa Lee; In Su Lee; Eun Yeol Lee
Journal of Industrial and Engineering Chemistry | 2012
Sung Hee Choi; Hee Sook Kim; Eun Yeol Lee
Applied Chemistry for Engineering | 2013
Yoon Ju Jo; Sung Hee Choi; Eun Yeol Lee
한국생물공학회 학술대회 | 2013
Sung Hee Choi; Jung Won Shin; Eun Yeol Lee
한국생물공학회 학술대회 | 2013
In Yeub Hwang; Sung Hee Choi; Yoon Ju Jo; Eun Yeol Lee
한국생물공학회 학술대회 | 2013
Sung Hee Choi; Eun Yeol Lee
한국생물공학회 학술대회 | 2013
Dong Hoon Hur; Sung Hee Choi; Ok Kyung Lee; Eun Yeol Lee
한국생물공학회 학술대회 | 2012
Yoon Ju Jo; Se Eun Yun; Sung Hee Choi; Eun Yeol Lee