Sung-Ook Hwang

Transactivation of bad by vorinostat-induced acetylated p53 enhances doxorubicin-induced cytotoxicity in cervical cancer cells

Vorinostat (VOR) has been reported to enhance the cytotoxic effects of doxorubicin (DOX) with fewer side effects because of the lower DOX dosage in breast cancer cells. In this study, we investigated the novel mechanism underlying the synergistic cytotoxic effects of VOR and DOX co-treatment in cervical cancer cells HeLa, CaSki and SiHa cells. Co-treatment with VOR and DOX at marginal doses led to the induction of apoptosis through caspase-3 activation, poly (ADP-ribose) polymerase cleavage and DNA micronuclei. Notably, the synergistic growth inhibition induced by the co-treatment was attributed to the upregulation of the pro-apoptotic protein Bad, as the silencing of Bad expression using small interfering RNA (siRNA) abolished the phenomenon. As siRNA against p53 did not result in an increase in acetylated p53 and the consequent upregulation of Bad, the observed Bad upregulation was mediated by acetylated p53. Moreover, a chromatin immunoprecipitation analysis showed that the co-treatment of HeLa cells with VOR and DOX increased the recruitment of acetylated p53 to the bad promoter, with consequent bad transactivation. Conversely, C33A cervical cancer cells containing mutant p53 co-treated with VOR and DOX did not exhibit Bad upregulation, acetylated p53 induction or consequent synergistic growth inhibition. Together, the synergistic growth inhibition of cervical cancer cell lines induced by co-treatment with VOR and DOX can be attributed to the upregulation of Bad, which is induced by acetylated p53. These results show for the first time that the acetylation of p53, rather than histones, is a mechanism for the synergistic growth inhibition induced by VOR and DOX co-treatments.

Abdominal Wall Metastasis of Uterine Papillary Serous Carcinoma in a Post-Menopausal Woman: A Case Report

Uterine papillary serous carcinoma (UPSC) is an aggressive form of endometrial cancer characterized by a high recurrence rate and poor prognosis. We report a case of a 58-year-old post-menopausal woman with an abdominal wall metastasis in stage IA UPSC. After surgical staging, she did not receive additional adjuvant therapy. An egg sized palpable mass developed in the right lower abdomen after 8 months. Both Abdominopelvic computed tomography (CT) and positron emission tomography (PET)-CT revealed a metastatic lesion in the abdominal wall. Hence, surgical excision was performed. The pathological findings showed metastatic UPSC with clear resection margin. After the diagnosis of UPSC metastasis in the abdominal wall, she received chemotherapy utilizing paclitaxel and carboplatin. After 3 years, no evidence of recurrence was found. Therefore, we suggest that even when UPSC is confined to the endometrium without lymph node metastasis and without lymphovascular invasion, chemotherapy should be considered as a postoperative adjuvant therapy.

A Frequency-Selecting Technique for Mobile Handset Antennas Based on Capacitance Switching

This paper introduces a mobile switchable antenna for long term evolution (LTE) of the 4th generation (4G), applicable to all mobile service bands. The validity of this antenna is assessed by design, realization, and measurement. A new frequency-selecting method is used in the proposed antenna, based on capacitance switching among four states. Due to the limited antenna space in existing terminals, it is quite di-cult to cover the entire low band with a single antenna. To overcome this di-culty, three single- pole double-throw (SPDT) switches, one 74HC04, and four capacitors are used. The resulting antenna covers the LTE (698{798MHz) and GSM (824{960MHz) bands in the low-band characteristic by realizing four capacitance states and at the same time covers the DCS (1710{1880 MHz)/PCS (1850{1990MHz)/WCDMA (1920{2170MHz) bands in the high-band characteristic. The antenna provides a gain of i1:04{6.00dBi, a radiation e-ciency of 32.73{74.99%, and omnidirectional characteristics in the H-plane. Because of this outstanding performance, it is expected that the new frequency - selecting technique will be applied in 4G mobile communication terminals.

Construction of the first compendium of chemical-genetic profiles in the fission yeast Schizosaccharomyces pombe and comparative compendium approach.

Genome-wide chemical genetic profiles in Saccharomyces cerevisiae since the budding yeast deletion library construction have been successfully used to reveal unknown mode-of-actions of drugs. Here, we introduce comparative approach to infer drug target proteins more accurately using two compendiums of chemical-genetic profiles from the budding yeast S. cerevisiae and the fission yeast Schizosaccharomyces pombe. For the first time, we established DNA-chip based growth defect measurement of genome-wide deletion strains of S. pombe, and then applied 47 drugs to the pooled heterozygous deletion strains to generate chemical-genetic profiles in S. pombe. In our approach, putative drug targets were inferred from strains hypersensitive to given drugs by analyzing S. pombe and S. cerevisiae compendiums. Notably, many evidences in the literature revealed that the inferred target genes of fungicide and bactericide identified by such comparative approach are in fact the direct targets. Furthermore, by filtering out the genes with no essentiality, the multi-drug sensitivity genes, and the genes with less eukaryotic conservation, we created a set of drug target gene candidates that are expected to be directly affected by a given drug in human cells. Our study demonstrated that it is highly beneficial to construct the multiple compendiums of chemical genetic profiles using many different species. The fission yeast chemical-genetic compendium is available at http://pombe.kaist.ac.kr/compendium.

The Correlation of cervical intraepithelial neoplasia 3 detected by cervical punch biopsies with the pathologic results of conization and hysterectomy

349 THE CORRELATION OF CERVICAL INTRAEPITHELIAL NEOPLASIA 3 DETECTED BY CERVICAL PUNCH BIOPSIES WITH THE PATHOLOGIC RESULTS OF CONIZATION AND HYSTERECTOMY Ki-Won Kim, MD, Kyoung-Mi Kim, MD, Jung-Woo Park, MD, Hyun-Jae Jung, MD, Jee-Young Han, MD, Joon-Mee Kim, MD, Jee-Hyun Park, MD, Sung-Ook Hwang, MD, Woo-Young Lee, MD, Eunseop Song, MD Departments of Obstetrics and Gynecology, Pathology, Inha University College of Medicine, Incheon, Korea

Simplified multiband front-end architecture using a multifunction circuit

A multifunction circuit to simplify a multiband front-end operating at very different bands is proposed. The multifunction circuit operates as a sub-harmonic mixer or an attenuator depending on a control voltage. A multiband front-end using the proposed multifunction circuit can reduce component, is 23 percent smaller, and is less expensive compared to a conventional architecture with a switching circuit.

Ovarian cancer with initial symptom of pulmonary embolism: A case report

Venous thromboembolism often occurs after major surgery and may occur as a consequence of underlying cancer. A 39-year-old woman presented to the emergency room with chief complaints of dyspnea and right chest pain. Chest computed tomography (CT) revealed pulmonary artery thromboembolism of the left lobe and a massive right pleural effusion. D-dimer level was 40.4 μg/mL. Abdomino-pelvic CT revealed a 15×12×14 cm solid and cystic mass in the pelvic cavity, suggesting ovarian cancer. A pleural biopsy found metastatic adenocarcinoma. She underwent cytoreductive surgery and pathologic findings revealed malignant mullerian mixed tumor of ovary. The hypercoagulable state in patients with ovarian cancer may occur as an initial symptom of pulmonary embolism. It is unresponsive to standard anticoagulation therapy. The hypercoagulable state in patient with ovarian cancer may be stopped by cytoreductive surgery of the malignancy.

Four risk of malignancy indices in evaluation of pelvic masses

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