Sung-Won Kang

Nutritional quality and physicochemical characteristics of defatted bovine liver treated by supercritical carbon dioxide and organic solvent

Defatted bovine liver (DBL) is a potential source of protein and minerals. Supercritical carbon dioxide (SC-CO2) and a traditional organic solvent method were used to remove lipid from bovine liver, and the quality characteristics of a control bovine liver (CBL), bovine liver defatted by SC-CO2 (DBLSC-CO2) at different pressures, and bovine liver defatted by organic solvent (DBL-OS) were compared. The DBLSC-CO2 samples had significantly higher (p<0.05) protein, amino acid, carbohydrate, and fiber contents than CBL and DBL-OS. There was a higher yield of lipid from CBL when using SC-CO2 than the organic solvent method. SDS-PAGE analysis demonstrated that the CBL and DBLSC-CO2 had protein bands of a similar intensity and area, whereas DBL-OS appeared extremely poor bands or no bands due to the degradation of proteins, particularly in the 50 to 75 kDa and 20 to 25 kDa molecular weight ranges. In addition, DBLSC-CO2 was shown to have superior functional properties in terms of total soluble content, water and oil absorption, and foaming and emulsification properties. Therefore, SC-CO2 treatment offers a nutritionally and environmentally friendly approach for the removal of lipid from high protein food sources. In addition, SC-CO2 may be a better substitute of traditional organic solvent extraction for producing more stable and high quality foods with high-protein, fat-free, and low calorie contents.

Amelioration of Trimethyltin-induced Cognitive Impairment in ICR Mice by Perilla Oil

This study aimed to investigate the anti-amnesic effect of perilla oil against trimethyltin (TMT)-induced learning and memory impairment in ICR mice. Perilla oil (2.5 mL/㎏ of body weight) and soybean oil (2.5 mL/㎏ of body weight) were administered orally to mice for 3 weeks, and at the end of the experimental period, cognitive behavior was examined by Y-maze and Morris water maze (MWM) tests. Behavioral tests showed that the mice treated with perilla oil had improved cognitive function compared to that in mice administered soybean oil. Analysis of brain tissue showed that perilla oil significantly lowered acetylcholinesterase activity and malondialdehyde (MDA) levels. Oxidized glutathione (GSH)-to-total GSH ratio also decreased from 10.4% to 5.3% in perilla oil-treated mice, but superoxide dismutase (SOD) activity increased from 11.7 to 14.2 U/㎎ protein. Therefore, these results suggest that the perilla oil could be a potential functional substance for improving cognitive function.S&T FOODS Co. LtdAbstract This study aimed to investigate the anti-amnesic effect of perilla oil against trimethyltin (TMT)-inducedlearning and memory impairment in ICR mice. Perilla oil (2.5 mL/kg of body weight) and soybean oil (2.5 mL/kg of bodyweight) were administered orally to mice for 3 weeks, and at the end of the experimental period, cognitive behavior wasexamined by Y-maze and Morris water maze (MWM) tests. Behavioral tests showed that the mice treated with perilla oilhad improved cognitive function compared to that in mice administered soybean oil. Analysis of brain tissue showed thatperilla oil significantly lowered acetylcholinesterase activity and malondialdehyde (MDA) levels. Oxidized glutathione(GSH)-to-total GSH ratio also decreased from 10.4% to 5.3% in perilla oil-treated mice, but superoxide dismutase (SOD)activity increased from 11.7 to 14.2 U/mg protein. Therefore, these results suggest that the perilla oil could be a potentialfunctional substance for improving cognitive function.Keywords: acetylcholinesterase, cognition, perilla oil, soybean oil