Sungchul Baek

Conducting polymer-hydrogels for medical electrode applications

Abstract Conducting polymers hold significant promise as electrode coatings; however, they are characterized by inherently poor mechanical properties. Blending or producing layered conducting polymers with other polymer forms, such as hydrogels, has been proposed as an approach to improving these properties. There are many challenges to producing hybrid polymers incorporating conducting polymers and hydrogels, including the fabrication of structures based on two such dissimilar materials and evaluation of the properties of the resulting structures. Although both fabrication and evaluation of structure–property relationships remain challenges, materials comprised of conducting polymers and hydrogels are promising for the next generation of bioactive electrode coatings.

Conductive Hydrogels: Mechanically Robust Hybrids for Use as Biomaterials

A hybrid system for producing conducting polymers within a doping hydrogel mesh is presented. These conductive hydrogels demonstrate comparable electroactivity to conventional conducting polymers without requiring the need for mobile doping ions which are typically used in literature. These hybrids have superior mechanical stability and a modulus significantly closer to neural tissue than materials which are commonly used for medical electrodes. Additionally they are shown to support the attachment and differentiation of neural like cells, with improved interaction when compared to homogeneous hydrogels. The system provides flexibility such that biologic incorporation can be tailored for application.

Development of bioactive conducting polymers for neural interfaces

Bioelectrodes for neural recording and neurostimulation are an integral component of a number of neuroprosthetic devices, including the commercially available cochlear implant, and developmental devices, such as the bionic eye and brain–machine interfaces. Current electrode designs limit the application of such devices owing to suboptimal material properties that lead to minimal interaction with the target neural tissue and the formation of fibrotic capsules. In designing an ideal bioelectrode, a number of design criteria must be considered with respect to physical, mechanical, electrical and biological properties. Conducting polymers have the potential to address the synergistic interaction of these properties and show promise as superior coatings for next-generation electrodes in implant devices.

Effects of dopants on the biomechanical properties of conducting polymer films on platinum electrodes

Conducting polymers have often been described in literature as a coating for metal electrodes which will dampen the mechanical mismatch with neural tissue, encouraging intimate cell interactions. However, there is very limited quantitative analysis of conducting polymer mechanics and the relation to tissue interactions. This article systematically analyses the impact of coating platinum (Pt) electrodes with the conducting polymer poly(ethylene dioxythiophene) (PEDOT) doped with a series of common anions which have been explored for neural interfacing applications. Nanoindentation was used to determine the coating modulus and it was found that the polymer stiffness increased as the size of the dopant ion was increased, with PEDOT doped with polystyrene sulfonate (PSS) having the highest modulus at 3.2 GPa. This was more than double that of the ClO4 doped PEDOT at 1.3 GPa. Similarly, the electrical properties of these materials were shown to have a size dependent behavior with the smaller anions producing PEDOT films with the highest charge transfer capacity and lowest impedance. Coating stiffness was found to have a negligible effect on in vitro neural cell survival and differentiation, but rather polymer surface morphology, dopant toxicity and mobility is found to have the greatest impact.

The biological and electrical trade-offs related to the thickness of conducting polymers for neural applications

Poly(3,4-ethylenedioxythiophene) (PEDOT) films have attracted substantial interest as coatings for platinum neuroprosthetic electrodes due to their excellent chemical stability and electrical properties. This study systematically examined PEDOT coatings formed with different amounts of charge and dopant ions, and investigated the combination of surface characteristics that were optimal for neural cell interactions. PEDOT samples were fabricated by varying the electrodeposition charge from 0.05 to 1 C cm(-2). Samples were doped with either poly(styrenesulfonate), tosylate (pTS) or perchlorate. Scanning electron micrographs revealed that both thickness and nodularity increased as the charge used to produce the sample was increased, and larger dopants produced smoother films across all thicknesses. X-ray photoelectron spectroscopy confirmed that the amount of charge directly corresponded to the thickness and amount of dopant in the samples. Additionally, with increased thickness and nodularity, the electrochemical properties of all PEDOT coatings improved. However, neural cell adhesion and outgrowth assays revealed that there is a direct biological tradeoff related to the thickness and nodularity. Cell attachment, growth and differentiation was poorer on the thicker, rougher samples, but thin, less nodular PEDOT films exhibited significant improvements over bare platinum. PEDOT/pTS fabricated with a charge density of <0.1Ccm(-2) provided superior electrochemical and biological properties over conventional platinum electrodes and would be the most suitable conducting polymer for neural interface applications.

Thin film hydrophilic electroactive polymer coatings for bioelectrodes

Hybrids of conducting polymers (CPs) and hydrogels have been explored as soft electroactive coatings for improving the mechanical and electrical performance of metallic implant electrodes. However, hydrogel fabrication methods pose a significant challenge to producing thin (sub-micron) coatings, resulting in bulky implants, which displace a large volume of tissue. To address this issue, polymer brushes of poly(2-hydroxyethyl methacrylate) (pHEMA) were covalently bound to a gold electrode using surface initiated atom-transfer radical-polymerization (SI-ATRP). The CP poly(3,4-ethylene dioxythiophene) (PEDOT) was electropolymersied through the brush layer to form a thin hydrophilic coating. The electrical properties of the hybrid were shown to be superior to homogenous CPs and the surface chemistry was varied as a function of PEDOT deposition time to present a graded composition of pHEMA and PEDOT. The resulting material was shown to support the attachment and differentiation of model neural cells, signifying the potential of these hybrid coatings for bioelectrode applications.

Nanoparticle-Assisted Heating Utilizing a Low-Cost White Light Source

In this experimental study, a filtered white light is used to induce heating in water-based dispersions of 20 nm diameter gold nanospheres (GNSs)—enabling a low-cost form of plasmonic photothermal heating. The resulting temperature fields were measured using an infrared (IR) camera. The effect of incident radiative flux (ranging from 0.38 to 0.77 W·cm−2) and particle concentration (ranging from 0.25–1.0 × 1013 particles per mL) on the solutions temperature were investigated. The experimental results indicate that surface heat treatments via GNSs can be achieved through complementary tuning of GNS solutions and filtered light.

Temperature Measurements of a Gold Nanosphere Solution in Response to Light-Induced Hyperthermia

Gold nanospheres (GNSs), biocompatible nanoparticles that can be designed to absorb visible and near-infrared light, have shown great potential in induced thermal treatment of cancer cells via Plasmonic Photothermal Therapy (PPTT) [3]. In this study, light induced heating of a water-based dispersion of 20 nm diameter GNSs was investigated at their plasmon resonance wavelength (λ = 520 nm). Temperature changes of the solution at the point of light irradiation were measured experimentally. A heat transfer model was used to verify the experimental data. The effect of two key parameters, light intensity and particle concentration, on the solution’s temperature was investigated. The experimental results showed a significant temperature rise of the GNS solution compared to de-ionized water. The temperature rise of GNS solution was linearly proportional to the concentration of GNS (from 0.25–1.0 C, C = 1×1013 particles per ml) and the light intensity (from 0.25 to 0.5 W cm−2). The experimental data matches the modeling results adequately. Overall, it can be concluded that the hyperthermic ablation of cancer cells via GNS can be achieved by controlled by the light intensity and GNS concentration. A novel component of this study is that a high power lamp source was used instead of a high power laser. This means that only low cost components were used in the current experimental set-up. Moreover, by using suitable filters and white light from the high power lamp source, it is possible to obtain light in many wavelength bands for the study of other nanoparticles with different plasmon wavelength ranges. The current results represtent just one example in this versatile experimental set-up developed. It should be noted, however, the plasmon resonance wavelength used in this study is not within the therapeutic window (750–1300 nm) [13]. Therefore, the GNSs used in this experiment are only applicable to the surface induced thermal treatment of cancer cells, for instance, in the skin.Copyright

Development of a Dynamic Testing Device for Predicting the Enhanced Permeation and Retention (EPR) Effect of Different Nanoparticles in Tumor Vessels

A microfluidic device was developed to simulate the dynamic conditions of the transvascular transport of nanoparticles. The device utilizes a microfluidic channel, filter paper, collagen gel—which represent the blood vessel, porous vessel wall, and interstitial matrix of the tumor, respectively. By controlling these components, the fluid-dynamic conditions of the tumor blood vessels can be simulated.For the initial study, Durapore® filters with the nominal diameter of 0.22 μm and 5 mg/ml type 1 collagen gel were used. The transvascular transport parameters of the membrane for a model particle, 20 nm gold spheres, were similar to those of rabbit VX2 carcinoma model. Overall, this design allows for fundamental research into the fluid dynamic transport of particles inside different organs, cancer types and stages. To investigate the physiological conditions of cancer, future studies will include modification of the filter membranes with proteins as well as subsequent culturing of endothelial cells on the filter and tumor cells in the gel matrix. Through this device, we will be able to prescribe nanoparticle fluids for to obtain enhanced permeation and retention.Copyright