Supatcharee Arun

Phenolic contents and antioxidant capacities of Thai-Makham Pom (Phyllanthus emblica L.) aqueous extracts *

概要通过对余甘子不同部位(叶、 枝和树皮)水提物的总酚含量的测定及体外抗氧化活性的研究, 为其体内及临床试验研究奠定了基础。 余甘子叶、 枝和树皮的水提物皆具有抗氧化活性, 其中树皮中的总酚含量最高, 抗氧化活性最强。

Changes of testicular phosphorylated proteins in response to restraint stress in male rats.

ObjectiveTo investigate male reproductive parameters via changes of potential testicular protein markers in restraint-stress rats.MethodsMale Sprague-Dawley rats were divided into two groups (non-immobilized control and restraint-immobilized/stress groups, n=8 each group). The stress animals were immobilized (12 h/d) by a restraint cage for 7 consecutive days. All reproductive parameters, morphology and histology were observed and compared between groups. In addition, the expression of steroidogenic acute regulatory (StAR) and phosphotyrosine proteins (previously localized in Sertoli and late spermatid cells) in testicular lysate was assayed by immuno-Western blotting.ResultsTestosterone level, sperm concentration and sperm head normality of stress rats were significantly decreased while the corticosterone level was increased as compared with the control (P<0.05). Histologically, stress rats showed low sperm mass in epididymal lumen and some atrophy of seminiferous tubules. Although the expression of testicular StAR protein was not significantly different between groups, changed patterns of the 131, 95, and 75 kDa testicular phosphorylated proteins were observed in the stress group compared with the control group. The intensity of a testicular 95-kDa phosphorylated protein was significantly decreased in stress rats.ConclusionsThis study has demonstrated the alteration of testicular phosphorylated protein patterns, associated with adverse male reproductive parameters in stress rats. It could be an explanation of some infertility in stress males.中文概要目 的寻找雄性大鼠应激模型中潜在的睾丸蛋白标记物,探索评估男性生殖能力新的参数。创新点压力可以影响男性生育能力,但其具体机制尚未明了。睾丸组织中酪氨酸磷酸化蛋白在精子形成过程中扮演着重要的角色。本研究首次利用动物模型说明了压力对睾丸组织中酪氨酸磷酸化蛋白表达的影响,进而探索评估男性生殖能力新的参数。方 法雄性SD 大鼠被随机分为对照组和束缚应激的实验组。实验组大鼠每天禁足12 小时,持续7 天。观察比较两组类固醇激素、精子密度、生殖系统形态学和组织学的差别,同时利用免疫印迹方法比较两组睾丸组织裂解产物中急性类固醇调节蛋白和酪氨酸的表达差别(早期在支持细胞中表达,后期在精子细胞中表达)。结 果实验组大鼠的睾酮水平和精子密度较对照组均显著降低,而精子畸形率和皮质酮显著升高。组织学比较发现实验组大鼠精子团数量减少并且出现输精管萎缩。两组大鼠睾丸组织中酪氨酸磷酸化蛋白的表达具有显著差异,实验组95 kDa 的磷酸化蛋白表达量显著降低,但两组急性类固醇调节蛋白的表达没有显著差异。结 论本研究证明束缚应激可使大鼠睾丸组织磷酸化蛋白表达降低,并相应伴随着生殖能力参数的降低。这也许可以从一个方面解释压力对男性不育的影响。

Antioxidant activity and protective effect of Clitoria ternatea flower extract on testicular damage induced by ketoconazole in rats

Background: Ketoconazole (KET), an antifungal drug, has adverse effects on the male reproductive system. Pre-treatments with antioxidant plant against testicular damage induced by KET are required. The flowers of Clitoria ternatea (CT) are proven to have hepatoprotective potential. However, the protective effect on KET-induced testicular damage has not been reported. Objective: To investigate the protective effect of CT flower extracts with antioxidant activity on male reproductive parameters including sperm concentration, serum testosterone level, histopathology of the testis, and testicular tyrosine phosphorylation levels in rats induced with KET. Methods: The antioxidant activity of CT flower extracts was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. Male rats were treated with CT flower extracts (10, 50, or 100 mg/kg BW) or distilled water via a gastric tube for 28 d (preventive period: Days 1–21) and induced by KET (100 mg/kg BW) via intraperitoneal injection for 7 d (induction period: Days 22–28). After the experiment, all animals were examined for the weights of the testis, epididymis plus vas deferens and seminal vesicle, serum testosterone levels, sperm concentration, histological structures and diameter of testis, and testicular tyrosine phosphorylation levels by immunoblotting. Results: The CT flower extracts had capabilities for DPPH scavenging and high reducing power. At 100 mg/kg BW, the extract had no toxic effects on the male reproductive system. Significantly, in CT+KET groups, CT flower extracts (50 and 100 mg/kg BW) alleviated the reduction of reproductive organ weight parameters, testosterone levels, and sperm concentration. In addition, CT flower extracts gave protection from testicular damage in KET-induced rats. Moreover, in the CT100+KET group, CT flower extracts significantly enhanced the expression of a testicular 50-kDa tyrosine phosphorylated protein compared with that of other groups. Conclusions: C. ternatea flower extracts possessing antioxidant activity are not harmful to the male reproductive system and can protect against testicular damage in KET-induced rats.

Phyllanthus emblica L. Branch Extract Ameliorates Testicular Damage in Valproic Acid-Induced Rats

El acido valproico (AVP) es utilizado frecuentemente en el tratamiento de pacientes epilepticos y puede danar los parametros reproductivos que causan la infertilidad masculina. Este estudio tuvo como objetivo investigar el efecto protector de la rama Phyllanthus emblica L. (PE) sobre el dano testicular de ratas inducidas con AVP. Ratas machos fueron divididas en 6 grupos (control, AVP, PE 250 mg/kg peso corporal, APV+ PE 50, 100, 250 mg/kg peso corporal, respectivamente). Los animales fueron pretratados con PE durante 23 dias y se administro AVP durante 10 dias antes de medir todos los parametros reproductivos. Los resultados mostraron que todas las dosis de PE protegen significativamente el peso y los niveles reducidos de testosterona testicular en ratas con AVP. El extracto de PE mejoro significativamente la concentracion de espermatozoides en ratas tratadas con AVP. Por otra parte, la histologia testicular de los grupos PE+AVP mostro disminucion de la histopatologia testicular en comparacion con el grupo tratado solo con AVP. Por lo tanto, parece que el extracto de la rama PE puede prevenir danos testiculares incluyendo los parametros reproductores masculinos en ratas inducidas con AVP.

Acute Effects of Mimosine Purified from Leucaena leucocephala on Male Reproductive System of Adult Mice

This study attempted to examine the acute effect of purified minosine extracted from Leucaena leucocephala on male reproductive system. Adults male mice were divided into 4 groups (n =8); control and 3 experimental groups treated with purifie d mimosine at different doses of 15, 30, and 60 mg/KgBW, respectively for 7 consecutive days. The morphological features and weights of bo dy and reproductive organs including testis, epididymis plus vas deferens, and seminal vesicle were compared among groups. In addition , epidi ymal sperm concentration and the changes of histopathology of testicular tissues in all groups were observed. The results showed tha t mimosine in all doses did not affect mice body weights. However, all doses of mimosine could significantly reduce the absolute and relative weights of testis and seminal vesicle but not of epididymis plus vas deferens. Significantly, mimosine at doses of 30, and 60 mg/KgBW coul d decrease sperm concentration. Moreover, the seminiferous atrophy and degeneration were obviously found in mimosine treated mice as compa r d to the control. In conclusion, consumption of Leucaena leucocephala edible parts containing mimosine could damage male reproductive organs which may cause acute male subfertility or infertility.