Suporn Nuchadomrong
Khon Kaen University
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Featured researches published by Suporn Nuchadomrong.
BMC Complementary and Alternative Medicine | 2013
Thanaset Senawong; Suwatchai Misuna; Somprasong Khaopha; Suporn Nuchadomrong; Prasan Sawatsitang; Chanokbhorn Phaosiri; Arpa Surapaitoon; Banchob Sripa
BackgroundThe rhizome of Hydnophytum formicarum Jack., a medicinal plant known in Thai as Hua-Roi-Roo, has been used in Thai traditional herbal medicine for treatment of cancer. We assessed the ability of its ethanolic and phenolic-rich extracts and its major phenolic compound, sinapinic acid, possessing histone deacetylase (HDAC) inhibitory activity to inhibit proliferation of 5 human cancer cell lines.MethodsHeLa cells were used to study HDAC inhibitory activity of the extracts, sinapinic acid, and a well-known HDAC inhibitor sodium butyrate. Five human cancer cell lines and one non-cancer cell line were used to study antiproliferative activities of the plant extracts, sinapinic acid and sodium butyrate, comparatively.ResultsResults indicated that ethanolic and phenolic-rich extracts of H. formicarum Jack. rhizome possessed both antiproliferative activity and HDAC inhibitory activity in HeLa cells. Sinapinic acid, despite its lower HDAC inhibitory activity than the well-known HDAC inhibitor sodium butyrate, inhibited the growth of HeLa and HT29 cells more effectively than sodium butyrate. However, sinapinic acid inhibited the growth of HCT116 and Jurkat cells less effectively than sodium butyrate. The non-cancer cell line (Vero cells) and breast cancer cell line (MCF-7 cells) appeared to be resistant to both sinapinic acid and sodium butyrate. The growth inhibitory effects of the ethanolic and phenolic-rich extracts and sinapinic acid in HeLa cells were mediated by induction of apoptosis.ConclusionsThe results of this study support the efficacy of H. formicarum Jack. rhizome ethanolic and phenolic-rich extracts for the treatment of cervical cancer, colon cancer, and T- cell leukemia in an alternative medicine. Further studies of other active ingredients from this plant are needed.
Food Chemistry | 2016
Chorkaew Aninbon; S. Jogloy; N. Vorasoot; A. Patanothai; Suporn Nuchadomrong; Thanaset Senawong
Terminal drought reduces pod yield and affected the phenolic content of leaves, stems and seed of peanut (Arachis hypogaea L.). The aim of this study was to investigate the effects of end of season water deficit on phenolic content in drought tolerant and sensitive genotypes of peanuts. Five peanut genotypes were planted under two water regimes, field capacity and 1/3 available water. Phenolic content was analyzed in seeds, leaves, and stems. The results revealed that terminal drought decreased phenolic content in seeds of both tolerant and sensitive genotypes. Phenolic content in leaves and stems increased under terminal drought stress in both years. This study provides basic information on changes in phenolic content in several parts of peanut plants when subjected to drought stress. Future studies to define the effect of terminal drought stress on specific phenolic compounds and antioxidant properties in peanut are warranted.
Breeding Science | 2017
Chorkaew Aninbon; S. Jogloy; N. Vorasoot; Suporn Nuchadomrong; C. Corley Holbrook; Craig Kvien; Naveen Puppala; A. Patanothai
Peanut seeds are rich in arginine, an amino acid that has several positive effects on human health. Establishing the genetic variability of arginine content in peanut will be useful for breeding programs that have high arginine as one of their goals. The objective of this study was to evaluate the variation of arginine content, pods/plant, seeds/pod, seed weight, and yield in Valencia peanut germplasm. One hundred and thirty peanut genotypes were grown under field condition for two years. A randomized complete block design with three replications was used for this study. Arginine content was analyzed in peanut seeds at harvest using spectrophotometry. Yield and yield components were recorded for each genotype. Significant differences in arginine content and yield components were found in the tested Valencia peanut germplasm. Arginine content ranged from 8.68–23.35 μg/g seed. Kremena was the best overall genotype of high arginine content, number of pods/plant, 100 seed weight and pod yield.
Bioscience, Biotechnology, and Biochemistry | 2015
Waraluk Senakoon; Suporn Nuchadomrong; Robin Y.-Y. Chiou; Gulsiri Senawong; S. Jogloy; Patcharin Songsri; A. Patanothai
This work revealed peanut seed prolamins likely displaying a defensive role besides the known nitrogen storage. Drought stress and proteomic approaches were used in varieties of peanuts to explore the prolamin member in association with a test against Aspergillus flavus spore germination. The stress effect was showed by aerial biomass, leaf content of malondialdehyde, and seed contamination by A. flavus. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis profiles were not informative for the antifungal polypeptides. From two-dimensional gel electrophoresis, the suspected polypeptides were those with pI 5.45–5.75 and sizes of 22.0–30.5 kDa specifically in Spanish-type peanuts. Regarding to the drought effect in most of these peanuts, the spot peak volume analysis deduced three novel prolamin-related antifungal polypeptides at pI 5.75–5.8 with 30.5, 27.5–28.5, and 22.0–22.5 kDa, which was confirmed after isoelectric purification at pH 5.60. The data could not yet conclude their correlation with resistance to drought and to seed infection by A. flavus. Graphical Abstract Antifungal peanut prolamins were related with polypeptides 1, 2, and 3 in pellet (Fig. A) not with spots 4, 5, and 6 in the supernatant (Fig. B) portion on precipitation at pH 5.60.
Parasitology International | 2017
Jeerati Prompipak; Thanaset Senawong; Khuanta Jokchaiyaphum; Kornpira Siriwes; Suporn Nuchadomrong; Thewarach Laha; Banchob Sripa; Gulsiri Senawong
Opisthorchis viverrini (Ov) infection is a long-time public health problem in Thailand that can lead to bile duct cancer, cholangiocarcinoma (CCA). Characterization of the Ov proteins at a molecular level will increase our knowledge of host-parasite interaction that can be applied to new drug, vaccine, or immunodiagnostic development. In this study, an important enzyme in the Ov glycolytic pathway, fructose-1,6-bisphosphate aldolase (FBPA), that had been obtained from a previous study was characterized and immunolocalized. The full-length sequence of OvFBPA gene is 1089bp and encodes 362 amino acids with a predicted molecular weight and isoelectric point of 39.54kDa and 7.61, respectively. Additionally, three OvFBPA isoforms were identified by sequence analysis. The amino acid sequence of OvFBPA-1 characterized in this study shared 98% identity to FBPA isoform 1 of Clonorchis sinensis that was classified based on highly conserved active residues to class-I FBPA. The recombinant OvFBPA-1 protein was expressed as a soluble form in Escherichia coli at 25°C with N-terminal His-tagged fusion protein and the purified OvFBPA-1 protein was used to generate polyclonal antibody in mice. Antibody against rOvFBPA-1 protein was able to detect the native OvFBPA-1 protein in both Ov infected hamster liver section and Ov excretory-secretory (ES) products by immunohistochemistry and western blotting, respectively.
European Food Research and Technology | 2011
Araya Saengkanuk; Suporn Nuchadomrong; S. Jogloy; A. Patanothai; Supalax Srijaranai
Plant Cell Reports | 2009
Nison Sattayasai; Runglawan Sudmoon; Suporn Nuchadomrong; Arunrat Chaveerach; Adelheid R. Kuehnle; Rasika G. Mudalige-Jayawickrama; Wandee Bunyatratchata
Archive | 2002
Rachanee Chaveerach; Hisato Kunitake; Suporn Nuchadomrong; Nison Sattayasai; Haruki Komatsu
Plant Foods for Human Nutrition | 2015
Bang-orn Srinameb; Suporn Nuchadomrong; S. Jogloy; A. Patanothai; Supalax Srijaranai
Acta Biochimica et Biophysica Sinica | 2008
Runglawan Sudmoon; Nison Sattayasai; Wandee Bunyatratchata; Arunrat Chaveerach; Suporn Nuchadomrong