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Dive into the research topics where Suradej Siripattanapipong is active.

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Featured researches published by Suradej Siripattanapipong.


International Journal for Parasitology | 2008

A suspected new species of Leishmania, the causative agent of visceral leishmaniasis in a Thai patient.

Theerayudh Sukmee; Suradej Siripattanapipong; Mathirut Mungthin; Jeerapun Worapong; Ram Rangsin; Yudhthana Samung; Wandee Kongkaew; Kusak Bumrungsana; Karoon Chanachai; Chamnan Apiwathanasorn; Pairaya Rujirojindakul; Somsak Wattanasri; Kumnun Ungchusak; Saovanee Leelayoova

A suspected new species of Leishmania is described as the causative agent of the third reported case of autochthonous visceral leishmaniasis in a Thai man living in Southern Thailand. The results of PCR-restriction fragment length polymorphism and sequence analysis of the internal transcribed spacer 1 of ssrRNA and the mini-exon genes were different from those of previously reported Leishmania species. A direct agglutination test (DAT) revealed that antibody against Leishmania infection was detected in nine domestic cats. No potential vectors could be identified. A large-scale epidemiological survey of leishmaniasis should be urgently conducted since visceral leishmaniasis is considered an emerging disease of public health concern in Thailand.


American Journal of Tropical Medicine and Hygiene | 2012

Autochthonous disseminated dermal and visceral leishmaniasis in an AIDS patient, southern Thailand, caused by Leishmania siamensis

Lertwut Bualert; Wiwat Charungkiattikul; Paramee Thongsuksai; Mathirut Mungthin; Suradej Siripattanapipong; Rommanee Khositnithikul; Tawee Naaglor; Christophe Ravel; Fouad El Baidouri; Saovanee Leelayoova

We report the first establishment of in vitro cultivation and genotypic characterization of Leishmania siamensis isolated from an autochthonous disseminated dermal and visceral leishmaniasis in a Thai acquired immunodeficiency syndrome (AIDS) patient. The molecular identification has shown that the parasite was identical to L. siamensis, a recently described Leishmania species reported in the southern provinces of Thailand. The phylogenetic analysis has confirmed L. siamensis as closely related to the zoonotic Leishmania species L. enrietti.


BMC Infectious Diseases | 2013

Sergentomyia (Neophlebotomus) gemmea , a potential vector of Leishmania siamensis in southern Thailand

Kobkan Kanjanopas; Suradej Siripattanapipong; Ubolrat Ninsaeng; Atitaya Hitakarun; Somnat Jitkaew; Preecha Kaewtaphaya; Peerapan Tan-ariya; Mathirut Mungthin; Chetsuda Charoenwong; Saovanee Leelayoova

BackgroundLeishmaniasis, caused by Leishmania siamensis, is an emerging disease in Thailand. Although reported cases have been increasing, epidemiological information of the disease including host and vector aspects is not clearly known. This study was a preliminary survey of the potential vector of L. siamensis in an affected area of leishmaniasis, Trang Province, southern Thailand.MethodsThe collection of sandflies was performed around the area where a case of leishmaniasis was reported using CDC light traps. Species of sandfly were identified based on morphological characteristics according to Lewis’s key. PCR amplification and sequencing of the heat shock protein 70 gene (hsp70) was used to identify L. siamensis DNA in sandflies.ResultsA total of 146 male and female sandflies were collected in the affected areas. Of 71 female sandflies, four species were identified, i.e., Sergentomyia (Neophlebotomus) gemmea, S. (Neophlebotomus) iyengari, S. (Parrotomyia) barraudi and Phlebotomus (Anaphlebotomus) stantoni. Among these species, S. (Neophlebotomus) gemmea was the most predominant species in all areas. DNA of L. siamensis was identified in S. (Neophlebotomus) gemmea. Nucleotide sequences of PCR products using DNA extracted from S. (Neophlebotomus) gemmea showed 99.8% identity to L. siamensis.ConclusionS. (Neophlebotomus) gemmea might be a potential vector of L. siamensis in an affected area, Trang Province, southern Thailand. However further studies are needed to prove whether these sandflies can be natural vectors of leishmaniasis.


Journal of Clinical Microbiology | 2005

Genotypic Study of Pneumocystis jirovecii in Human Immunodeficiency Virus-Positive Patients in Thailand

Suradej Siripattanapipong; Jeerapun Worapong; Mathirut Mungthin; Saovanee Leelayoova; Peerapan Tan-ariya

ABSTRACT Pneumocystis jirovecii is one of the common opportunistic infections in human immunodeficiency virus (HIV)-infected patients in Thailand. Information regarding genotypic and epidemiological of this organism in Thai patients is not available. We analyzed the genotypes of 28 P. jirovecii-positive specimens from bronchoalveolar lavage and sputum samples from HIV-infected Thai patients based on nucleotide variations of the internal transcribed spacer regions 1 and 2 of the rRNA gene. Thirteen genotypes were the same as previously reported outside Thailand. Ten genotypes, which included Bp, Er, Eq, Ic, Ir, Ip, Rc, Rp, Qb, and Qq, were new. Ir and Rp were unique and dominant types observed in HIV-infected Thai patients. Thirteen specimens (46.4%) were infected with a single type of P. jirovecii, and fifteen (53.6%) were mixed infections. These differences may be used as genotypic markers for studying the epidemiology and transmission of P. jirovecii in the Thai population.


BMC Microbiology | 2013

Multilocus characterization and phylogenetic analysis of Leishmania siamensis isolated from autochthonous visceral leishmaniasis cases, southern Thailand

Saovanee Leelayoova; Suradej Siripattanapipong; Atitaya Hitakarun; Hirotomo Kato; Peerapan Tan-ariya; Padet Siriyasatien; Seksit Osatakul; Mathirut Mungthin

BackgroundVisceral leishmaniasis (VL) caused by Leishmania siamensis is an emerging disease continuously reported in six southern provinces of Thailand. To date, the phylogenetic relationships among Leishmania isolates from Thai patients and other Leishmania species are still unclear and the taxonomic diversity needs to be established. In this study, the phylogenetic inference trees were constructed based on four genetic loci (i.e., SSU-rRNA, ITS1, hsp70, and cyt b), using DNA sequences obtained from autochthonous VL patients from southern Thailand and reference sequences of reported Leishmania isolates from other studies deposited in GenBank.ResultsPhylogenetic analyses of hsp70 and cyt b loci supported a clade comprised of L. siamensis isolates, which is independent to the other members in the genus Leishmania. In combination with genetic distance analysis, sequence polymorphisms were observed among L. siamensis isolates and two different lineages could be differentiated, lineages PG and TR. Phylogenetic analysis of the cyt b gene further showed that L. siamensis lineage TR is closely related to L. enrietti, a parasite of guinea pigs.ConclusionThe finding of this study sheds further light on the relationships of L. siamensis, both in intra- and inter-species aspects. This information would be useful for further in-depth studies on the biological properties of this important parasite.


American Journal of Tropical Medicine and Hygiene | 2013

Identification of Blastocystis subtype 1 variants in the Home for Girls, Bangkok, Thailand.

Umaporn Thathaisong; Suradej Siripattanapipong; Mathirut Mungthin; Duangnate Pipatsatitpong; Peerapan Tan-ariya; Tawee Naaglor; Saovanee Leelayoova

A cross-sectional study of Blastocystis infection was conducted to evaluate the prevalence, risk factors, and subtypes of Blastocystis at the Home for Girls, Bangkok, Thailand in November 2008. Of 370 stool samples, 118 (31.9%) were infected with Blastocystis. Genotypic characterization of Blastocystis was performed by polymerase chain reaction and sequence analysis of the partial small subunit ribosomal RNA (SSU rRNA) gene. Subtype 1 was the most predominant (94.8%), followed by subtype 6 (3.5%) and subtype 2 (1.7%). Sequence analyses revealed nucleotide polymorphisms for Blastocystis subtype 1, which were described as subtype 1/variant 1, subtype 1/variant 2. Blastocystis subtype 1/variant 1 was the most predominant infection occurring in almost every house. The results showed that subtype analysis of Blastocystis was useful for molecular epidemiological study.


Parasites & Vectors | 2014

Comparison of PCR methods for detection of Leishmania siamensis infection

Atitaya Hitakarun; Peerapan Tan-ariya; Suradej Siripattanapipong; Mathirut Mungthin; Phunlerd Piyaraj; Tawee Naaglor; Padet Siriyasatien; Saruda Tiwananthagorn; Saovanee Leelayoova

BackgroundLeishmania siamensis, a newly identified species, has been reported as a causative agent of leishmaniasis in Thailand. This organism has been identified and genetically characterized using PCR techniques based on several target genes. However, the sensitivities and specificities of these methods for the diagnosis of L. siamensis infection have never been evaluated.MethodsTo evaluate the sensitivities and specificities of PCR methods to detect L. siamensis infection, PCR for different genetic markers, i.e., the small subunit ribosomal RNA region (SSU-rRNA), the internal transcribed spacer 1 region (ITS1), cysteine protease B (cpb), cytochrome b (cyt b), heat shock protein 70 (hsp 70), the spliced leader mini-exon, and the triose-phosphate isomerase (tim) genes were compared.ResultsBoth the ITS1-PCR and the SSU rRNA-PCR could detect promastigote of L. siamensis at concentrations as low as 0.05 parasites/μl or the DNA concentration at 2.3 pg/μl. Though the ITS1-PCR method only recognized 8 samples as positive, all of these could be assessed as true positive according to microscopic diagnosis and/or amplifying the results of the PCR and their sequencing, while other methods also produced false positive results. Compared with the ITS1-PCR method, the PCR amplified SSU-rRNA and cpb gene showed 100% sensitivity for the detection of L. siamensis in clinical specimens. The PCR amplified mini-exon and hsp 70 gene also gave a high sensitivity of 87.5%. In contrast, the PCR methods for cyt b and tim gene showed low sensitivity. The PCR methods for cyt b, mini-exon and tim gene showed 100% specificity compared with the ITS1-PCR.ConclusionAs a result, the ITS1-PCR method is a suitable target for PCR-based detection of L. siamensis infection in clinical specimens due to its high sensitivity and specificity. The results of this study can be used for molecular diagnosis as well as in epidemiological studies of L. siamensis in affected areas.


American Journal of Tropical Medicine and Hygiene | 2014

Recurrences of visceral leishmaniasis caused by Leishmania siamensis after treatment with amphotericin B in a seronegative child.

Seksit Osatakul; Mathirut Mungthin; Suradej Siripattanapipong; Atitaya Hitakarun; Rommanee Kositnitikul; Tawee Naaglor; Saovanee Leelayoova

We report the first case of visceral leishmaniasis caused by Leishmania siamensis in a seronegative child. She was treated with amphotericin B at 1 mg/kg/day for 3 weeks; however, recurrences occurred twice. The patient was cured after the administration of amphotericin B for 5 weeks and monthly prophylaxis for 6 months.


Emerging Infectious Diseases | 2018

Visceral Leishmaniasis in Traveler to Guyana Caused by Leishmania siamensis, London, UK

Saovanee Leelayoova; Suradej Siripattanapipong; Mathirut Mungthin

described L. siamensis strains, except 1, are now reported as L. martiniquensis. Their rDNA internal transcribed spacer 1 sequences are still deposited in GenBank under the name L. siamensis. The exception, reported from Thailand (GenBank accession no. JX195640), is the only known L. siamensis sample to date. New analysis of Leishmania (Mundinia) sequences available in GenBank and of L. infantum showed no variability in L. martiniquensis, including the sequence (GenBank accession no. LT577674) reported by Polley et al. (1), and sequence divergence when compared with L. siamensis (32.4%), a Leishmania sp. from Ghana (32.3%) (4), L. enrietti (30.6%), and L. infantum (43.6%). L. martiniquensis has been reported worldwide (Florida, West Indies, central Europe, and Southeast Asia). However, L. siamensis has been reported only once (in Thailand). If one considers possible quiescence of the parasite, and that the patient was from Guyana, migrated to the United Kingdom in 1967, and had a relevant travel history, including visits to France (2003), Ghana (2005), Caribbean Grenada (2012), and Guyana (2012 and 2013), the geographic origin of this infection is unknown. Moreover, the mode of transmission of L. martiniquensis is not yet clearly defined. In contrast to the report of Polley et al. (1), although the genus Sergentomyia could play a role in some foci of leishmaniasis, it has never been recorded in the Americas (5).


American Journal of Tropical Medicine and Hygiene | 2008

Drinking Water: A Possible Source of Blastocystis spp. Subtype 1 Infection in Schoolchildren of a Rural Community in Central Thailand

Saovanee Leelayoova; Suradej Siripattanapipong; Umaporn Thathaisong; Tawee Naaglor; Paanjit Taamasri; Phunlerd Piyaraj; Mathirut Mungthin

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Mathirut Mungthin

Phramongkutklao College of Medicine

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Saovanee Leelayoova

Phramongkutklao College of Medicine

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Tawee Naaglor

Phramongkutklao College of Medicine

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Paanjit Taamasri

Phramongkutklao College of Medicine

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Parima Boontanom

Phramongkutklao College of Medicine

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Phunlerd Piyaraj

Phramongkutklao College of Medicine

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