Suresh Lokhande

Nitrogen Concentration and Dry-Matter Accumulation in Maize Crop: Assessing Maize Nitrogen Status with an Allometric Function and a Chlorophyll Meter

The ability to determine the optimal nitrogen (N) content in maize plants needed to obtain maximum growth is important to the management of the crop. It has been shown that N content declines as a function of aboveground biomass accumulation (W): [N] = 3.4W–0.37. The goal of this study is to evaluate the applicability of relating chlorophyll meter readings with the optimal N content relationship to provide a tool for whole-plant N-status diagnosis in maize without the necessity of measuring N content. Biomass of shoot and specific organs, N concentration, and chlorophyll meter measurement of specific leaves were measured over several sites and years. Nitrogen-concentration measurements indicated that whole-plant N status can be represented by the N concentration of the topmost fully expanded leaf. A quantitative relationship between N concentration and chlorophyll meter measurement on the uppermost expanded leaf was established and validated.

Genotypic variability among cotton cultivars for heat and drought tolerance using reproductive and physiological traits

Development of rapid and inexpensive screening tools for heat and drought stress tolerance is needed and will be helpful in cotton breeding programs and selecting cultivars for a niche environment. In this study, several pollen-based traits at optimum and high temperatures and physiological parameters measured during the boll-filling period were used to evaluate variability among the cultivars for heat and drought stresses. Principal component analysis and drought stress response index methods were used to categorize cotton cultivars into three heat and drought tolerant clusters. Based on the combined analysis, PX532211WRF has been identified as heat- and drought-tolerant, and would be expected to perform better under both heat- and drought-stressed environments. A poor correlation between reproductive and physiological indices indicates that screening breeders have to use different traits to screen cultivars for reproductive and vegetative tolerance. Identified traits could serve as valuable screening tools in cotton breeding programs aimed at developing genotypes to a changing climate. Moreover, cultivar-dependent relative scores will aid in the identification of cultivars best suited to niche environments to alleviate the influences of abiotic stresses at both vegetative and reproductive stages.