Susan A. Kaplan

Molecular markers in keratins from Mysticeti whales for species identification of baleen in museum and archaeological collections

Baleen has been harvested by indigenous people for thousands of years, as well as collected by whalers as an additional product of commercial whaling in modern times. Baleen refers to the food-filtering system of Mysticeti whales; a full baleen rack consists of dozens of plates of a tough and flexible keratinous material that terminate in bristles. Due to its properties, baleen was a valuable raw material used in a wide range of artefacts, from implements to clothing. Baleen is not widely used today, however, analyses of this biomolecular tissue have the potential to contribute to conservation efforts, studies of genetic diversity and a better understanding of the exploitation and use of Mysticeti whales in past and recent times. Fortunately, baleen is present in abundance in museum natural history collections. However, it is often difficult or impossible to make a species identification of manufactured or old baleen. Here, we propose a new tool for biomolecular identification of baleen based on its main structural component alpha-keratin (the same protein that makes up hair and fingernails). With the exception of minke whales, alpha-keratin sequences are not yet known for baleen whales. We therefore used peptide mass fingerprinting to determine peptidic profiles in well documented baleen and evaluated the possibility of using this technique to differentiate species in baleen samples that are not adequately identified or are unidentified. We examined baleen from ten different species of whales and determined molecular markers for each species, including species-specific markers. In the case of the Bryde’s whales, differences between specimens suggest distinct species or sub-species, consistent with the complex phylogeny of the species. Finally, the methodology was applied to 29 fragments of baleen excavated from archaeological sites in Labrador, Canada (representing 1500 years of whale use by prehistoric people), demonstrating a dominance of bowhead whale (Balaena mysticetus) in the archaeological assemblage and the successful application of the peptide mass fingerprinting technique to identify the species of whale in unidentified and partially degraded samples.

Living on the Edge: Inughuit Women and Geography of Contact

The women of northwestern Greenland experienced contact with Euro-American men in multiple ways and in a variety of geographical contexts. Together the archaeological record and unpublished historical documents reveal the complexities of these situations and women’s responses to them. Archaeological and documentary data from two early 20th-century contact situations at Iita in northwestern Greenland and Floeberg Beach on Ellesmere Island, Nunavut, highlight the varied nature of women’s responses to contact and the ways in which interaction with newcomers affected them individually and collectively. The women’s responses varied over time and across space. They responded to both the stresses and the opportunities of contact with resilience and resistance, acceptance and rejection, depending on circumstances.