Susan Firestone

Life-threatening Apnea in Infants Recovering from Anesthesia

To determine whether prematurely born infants with a history of idiopathic apneic episodes are more prone than other infants to life-threatening apnea during recovery from anesthesia, the authors prospectively studied 214 infants (173 full term, 41 premature) who received anesthesia. Fifteen premature infants had a preanesthetic history of idiopathic apnea. Six of these required mechanical ventilation because of idiopathic apneic episodes during emergence from anesthesia. Two were ventilated for other reasons, and seven recovered normally. Infants ventilated for apnea were younger (postnatal age 1.6 +/- 1.2 months, mean +/- SD; conceptual age 38.6 +/- 3.0 weeks) than those who recovered normally (postnatal age 5.6 +/- 2.7 months; conceptual age 55.1 +/- 11.3 weeks) (P less than 0.01). No other premature or full-term infant was ventilated because of postoperative apneic episodes. The authors conclude that anesthetics may unmask a defect in ventilatory control of prematurely born infants younger than 41-46 weeks conceptual age who have a preanesthetic history of idiopathic apnea.

Isoflurane's Enhancement of Chloride Flux through Rat Brain γ-Aminobutyric Acid Type A Receptors Is Stereoselective

Background Recent evidence is consistent with the view that volatile anesthetics interact directly with excitable membrane-bound channel proteins. If these agents interact directly with chiral centers in the neuronal cell membrane, then their effects should be stereoselective. Using rat brain membranes enriched in gamma-aminobutyric acid type A (GABA sub A) receptors, we investigated the hypothesis that the permeability response of this well-characterized central nervous system channel protein to stereoisomers of isoflurane is stereoselective.

Intraoperative events diagnosed by expired carbon dioxide monitoring in children

Expired carbon dioxide measurements (PECO2) were used (1) to assess the adequacy of initial alveolar ventilation, and (2) to document intraoperative airway events and metabolic trends. Three hundred and thirty-one children were studied. Thirty-five intraoperative events were diagnosed by continuous PeCO2 monitoring; 20 were potentially life-threatening problems (malignant hyperthermia, circuit disconnection or leak, equipment failure, accidental extubation, endobronchial intubation, or kinked tube); only two of these were also diagnosed clinically. The duration of anaesthesia may be a factor: 3.9 hours for cases with events vs. 2.5 hours for cases without events (p < 0.002). There was a higher incidence of hypercarbia (peak expired PeCO2≥ 50) in children who were not intubated (29 per cent) compared to those who had an endotracheal tube in place (12 per cent) (p = 0.0001). Hypocarbia (peak expired PeCO2≤30) was more frequent in intubated cases (11 per cent) than in unintubated cases (three per cent) (p = 0.03). There was a high incidence of hypocarbia in infants less than one year of age (p = 0.02). We conclude: (1) lifethreatening airway problems are common during anaesthesia in paediatric patients; (2) quantitative measurement of PECO2 provides an early warning of potentially catastrophic anaesthetic mishaps; (3) the incidence of events increases with duration of anaesthesia.RésuméL’6tude du CO2 en fin dexpiration (PeCO2) a été utilisée afin d’évaluer (1) la fonction respiratoire initiate et (2) pour documenter les événemenls per-opératoires touchant les voies aériennes ainsi que les changements métaboliques. 331 enfants ont été étudiés. 35 événements per-opératoires ont été diagnostiqués par une surveillance constante de la PeCO2; 20 représentaient des problèmes mettant en danger la vie (hyperthermie maligne, disconnection de circuit, fuite, bris d’équipement, extubation accidentelle, intubation endobronchique, ou tube endotrachéal coudd); seulement deux de ces événements ont été aussi diagnostiqués cliniquement. La durée de l’anesthésie pouvait aussi être un facteur: les présentants les accidents ont duré en moyenne 3.9 heurs contre 2.5 heures pour les cas n’ayant pas présenté de problèmes (p < 0.002). Il y avait un incidence plus élevée d’hypercarbie (PECO2 ≥ 50,) chez les enfants qui n’étaient pas intubés (29 pour cent) a comparé à ceux dont le tube endotrachéal élail en place (12 pour cent) (p = 0.0001). L’hypocarbie (PeCO2 ≤ 30) était plus fréquente chez les patients intubés (11 pour cent) que chez ceux qui n’étaient pas intubés (trois pour cent) (p = 0.03). It y avait une incidence plus grande d’hypocarbie chez les enfants âgés de moins qu’un an (p = 0.02). On colclut: 1) les problemes de voies aeriennes pouvant mettre en danger la vie sont fréquents lors de I’anesthésie pédiatrique; 2) la mesure quantitative de la PeCO2 fournie un signal d’alarme précoce pour les accidents anesthésiques potentiellement catastrophiques; 3) l’incidence des accidents augmente avec le temps.

Staurosporine, a Protein Kinase C Inhibitor, Decreases the General Anesthetic Requirement in rana pipiens Tadpoles

Protein kinase C, the intracellular effector for the inositol phosphate-mediated signal transduction pathway, plays a key role in neurotransmission in the central nervous system. Although the in vitro activity of protein kinase C is inhibited by therapeutic concentrations of volatile anesthetics, the relation of this effect to in vivo obtundation has not been established. If obtundation by volatile anesthetics involves protein kinase C inhibition, then an inhibitor of this enzyme should decrease the anesthetic requirement. To test this hypothesis, we compared the EC50s of halothane and diethylether for loss of the righting reflex in Rana pipiens tadpoles pretreated with Staurosporine and in untreated controls. Anesthetic concentrations were confirmed by gas chromatography and Staurosporine concentrations by ultraviolet absorbance spectropho-tometry. Results obtained in more than 1000 animals indicated that pretreatment with Staurosporine concentrations in the nanomolar range significantly decreased the EC50 for both halothane (68% of control; P < 0.035) and diethylether (41% of control; P < 0.001). This finding implies that protein kinase C inhibition may play a role in general anesthetic-induced obtundation.