Susana Mendes

Cytoprotective effect of seaweeds with high antioxidant activity from the Peniche coast (Portugal)

Screening of antioxidant potential of dichloromethane and methanolic extracts of twenty-seven seaweeds from the Peniche coast was performed by: total phenolic contents (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and oxygen radical absorbance capacity (ORAC). Seaweeds revealing the highest antioxidant activity were screened for cytoprotective potential in MCF-7 cells, including the mitochondrial membrane potential analysis and the caspase-9 activity. High correlation was found between TPC of seaweed extracts and their scavenging capacity on DPPH and peroxyl radicals. The highest antioxidant activity was displayed by the methanolic fraction of brown seaweeds belonging to Fucales, however Ulva compressa presented the highest cytoprotective effect by blunting the apoptosis process. These results suggest that high antioxidant activity may not be directly related with high cytoprotective potential. Thus, seaweeds reveal to be a promising source of compounds with potential against oxidative stress.

Effects of sewage pollution on the structure of rocky shore macroinvertebrate assemblages

The urgency to find efficient indices and indicators to prevent further deterioration of coastal areas is one of the hot topics in today’s scientific publication. However, a detailed knowledge of community responses to anthropogenic impacts is essential to sustain those indices. The studies on the response of benthic community to sewage pollution on intertidal rocky shores are generally based on visual census and do not take into account the tidal levels. In order to fulfil this gap in this study: (i) the sampling was performed by destructive sampling, with all individuals identified to the species level; (ii) the sampling was done at all levels of the intertidal (sublittoral fringe, eulittoral, and littoral fringe). Sewage pollution changed the environmental variables and the abundance of macroinvertebrates, being Mytilus galloprovincialis, Melarhapheneritoides, and Chthamalus montagui the species most responsible for the dissimilarities observed. Effects were different on the three intertidal zones: community structure changed in the sublittoral fringe; suspension-feeders abundances and species richness increased in the eulittoral; no differences were detected in the littoral fringe. Moreover, the results confirm that the presence of sewage discharges tended to benefit suspension feeders, and that the sensitive species were replaced by opportunistic ones.

Effects of ocean acidification on the swimming ability, development and biochemical responses of sand smelt larvae.

Ocean acidification, recognized as a major threat to marine ecosystems, has developed into one of the fastest growing fields of research in marine sciences. Several studies on fish larval stages point to abnormal behaviours, malformations and increased mortality rates as a result of exposure to increased levels of CO2. However, other studies fail to recognize any consequence, suggesting species-specific sensitivity to increased levels of CO2, highlighting the need of further research. In this study we investigated the effects of exposure to elevated pCO2 on behaviour, development, oxidative stress and energy metabolism of sand smelt larvae, Atherina presbyter. Larvae were caught at Arrábida Marine Park (Portugal) and exposed to different pCO2 levels (control: ~600μatm, pH=8.03; medium: ~1000μatm, pH=7.85; high: ~1800μatm, pH=7.64) up to 15days, after which critical swimming speed (Ucrit), morphometric traits and biochemical biomarkers were determined. Measured biomarkers were related with: 1) oxidative stress - superoxide dismutase and catalase enzyme activities, levels of lipid peroxidation and DNA damage, and levels of superoxide anion production; 2) energy metabolism - total carbohydrate levels, electron transport system activity, lactate dehydrogenase and isocitrate dehydrogenase enzyme activities. Swimming speed was not affected by treatment, but exposure to increasing levels of pCO2 leads to higher energetic costs and morphometric changes, with larger larvae in high pCO2 treatment and smaller larvae in medium pCO2 treatment. The efficient antioxidant response capacity and increase in energetic metabolism only registered at the medium pCO2 treatment may indicate that at higher pCO2 levels the capacity of larvae to restore their internal balance can be impaired. Our findings illustrate the need of using multiple approaches to explore the consequences of future pCO2 levels on organisms.

Trace Elements in Edible Rocky Shore Species: Effect of Sewage Discharges and Human Health Risk Implications

ABSTRACT Sewage pollution is a worldwide concern and can result in increasing levels of trace elements in the environment that can pose serious risks both to wildlife and human health. This highlights the importance of studying the role of sewage discharges in the contamination of aquatic systems, especially in rocky shores, since part of our food resources is directly collected from coastal waters. For this purpose, the accumulation of trace elements (Cu, Zn, Pb, Ni, Co, Cd, Fe, Mn, and As) by edible molluscs (Mytilus galloprovincialis, Patella ulyssiponensis, and Phorcus lineatus) was compared between one sewage-impacted area and two reference areas. This study suggests that the concentrations of trace elements in the soft tissues of the selected molluscs can be affected by the presence of sewage discharges, and that limpets seem to be the best bioindicator. Moreover, the sewage pollution increased the concentrations of As in the mollusc species, emphasizing its potential damaging effects on natural systems and on edible species.

Effect of stocking density and different diets on growth of Percula Clownfish, Amphiprion percula (Lacepede, 1802)

The aim of this study was to evaluate the influence of stocking density (0.5, 1, 2 and 3 fishL−1) and commercial marine fish diets (diet A, B, C and D) over four months on specific growth rate, condition factor, percentage without anomalous pigmentation (partial or total lack of white bands -miss-band) and survival of juvenile Amphiprion percula.Results showed that at 0.5 fishL−1 densities induced the best survival (100%) and also the maximum percentage of fish without miss-band (58.33 +/−4.417%). The maximum SGR was obtained for the 0.5 fishL−1 (0.459 ± 0.023% cm/day). However, the best condition factor (2.53 +/− 0.27) was achieved for 2 fishL−1 densities. There were no significant differences in survival (68.9 to 84.5%), fish without miss-bands (18.03 to 26.92%) and condition factor (1.92 to 2.1) among diets during the experimental period. On the other hand, diet C (with 41% crude protein) supported the best SGR (0.485 ± 0.001% cmday−1).The results suggested that stocking density are critical and more relevant when compared with the different diet tested, namely on specific growth rate, condition factor, the miss-band and survival of juvenile percula clownfish.This study has particular significance with regards to anemonefishes husbandry in terms of survival and production efficiency.

Arsenic accumulation in intertidal macroalgae exposed to sewage discharges

Arsenic is a widely distributed element in marine ecosystems. The main anthropogenic sources of this contaminant are domestic and industrial wastewaters, and since it can be harmful to humans even at low concentrations, it has been ranked as one of the top hazardous substances. Therefore, the analysis of arsenic is an essential task to assess the potential environmental and human health risk associated with sewage discharges. The accumulation of total arsenic on intertidal rocky shore macroalgae exposed to contaminated sewage discharges was measured in five macroalgae species (Asparagopsis armata, Codium sp., Plocamium cartilagineum, Saccorhiza polyschides and Ulva sp.). Differences in the concentrations of arsenic were examined in the seawater and in the macroalgae species. The results showed significantly higher concentrations of arsenic near the sewage discharges in all the species except S. polyschides. Although the information obtained from total arsenic determination is not enough to assess the toxicological risk in the environment, this paper gives an important contribution on contamination risks and helps in choosing potential good biomonitors.

CO-tucker: a new method for the simultaneous analysis of a sequence of paired tables

ABSTRACT Relationships between species and their environment are a key component to understand ecological communities. Usually, this kind of data are repeated over time or space for communities and their environment, which leads to a sequence of pairs of ecological tables, i.e. multi-way matrices. This work proposes a new method which is a combined approach of STATICO and Tucker3 techniques and deals to the problem of describing not only the stable part of the dynamics of structure–function relationships between communities and their environment (in different locations and/or at different times), but also the interactions and changes associated with the ecosystems’ dynamics. At the same time, emphasis is given to the comparison with the STATICO method on the same (real) data set, where advantages and drawbacks are explored and discussed. Thus, this study produces a general methodological framework and develops a new technique to facilitate the use of these practices by researchers. Furthermore, from this first approach with estuarine environmental data one of the major advantages of modeling ecological data sets with the CO-TUCKER model is the gain in interpretability.

An empirical comparison of Canonical Correspondence Analysis and STATICO in the identification of spatio-temporal ecological relationships

The wide-ranging and rapidly evolving nature of ecological studies mean that it is not possible to cover all existing and emerging techniques for analyzing multivariate data. However, two important methods enticed many followers: the Canonical Correspondence Analysis (CCA) and the STATICO analysis. Despite the particular characteristics of each, they have similarities and differences, which when analyzed properly, can, together, provide important complementary results to those that are usually exploited by researchers. If on one hand, the use of CCA is completely generalized and implemented, solving many problems formulated by ecologists, on the other hand, this method has some weaknesses mainly caused by the imposition of the number of variables that is required to be applied (much higher in comparison with samples). Also, the STATICO method has no such restrictions, but requires that the number of variables (species or environment) is the same in each time or space. Yet, the STATICO method presents information that can be more detailed since it allows visualizing the variability within groups (either in time or space). In this study, the data needed for implementing these methods are sketched, as well as the comparison is made showing the advantages and disadvantages of each method. The treated ecological data are a sequence of pairs of ecological tables, where species abundances and environmental variables are measured at different, specified locations, over the course of time.

It is All about Safety: An Experience in Pico Mountain—Portugal

ABSTRACT Mountain adventure tourism represents 20% of the current tourism flows and will continue to grow, and, consequently there is a great need to better understand this segment. Pico Mountain, a mountain destination, in Portugal has followed this growth trend; however the lack of knowledge about this phenomenon is still a reality. Therefore, our goal is to define the Pico Mountain visitor’s profile as well as to assess the importance of the mountain guides’ roles in the Pico climbing. To achieve this goal a quantitative analysis was employed based on a survey applied in the territory. Our main results indicated that visitors of Pico Mountain are mainly young, European, inexperienced, and “package” adventure tourists that undertake “soft” adventure activities. For 90% of the respondents the mountain guides played an important role in the trip as well as great influence on it. Furthermore, the feeling of safety assured by guides is the most valued characteristic of mountain guides for all the respondents and it is the main reason why they are hired.

Reproductive cycle of the European clam Ruditapes decussatus from Óbidos Lagoon, Leiria, Portugal

ABSTRACT In Portugal, the European clam (Ruditapes decussatus) is an important commercial resource. Óbidos Lagoon is a strong candidate as a cultivation area to increase European clam exploitation. However, the reproductive biology of this population has not been described. In this work, the reproductive cycle of R. decussatus was characterized by determining gonadal development stages, gonad index, condition index, and biochemical composition. The relationship between reproduction and environmental parameters (sea surface temperature, chlorophyll a, and particulate organic matter) was assessed. Ruditapes decussatus had an annual reproductive cycle. The gametogenic cycle started in late winter, and the ripe stage in spring was followed by spawning that began at the end of spring/early summer and extended until early autumn. The subsequent period of sexual rest occurred during the winter. Condition index showed seasonal variations related to food availability (chlorophyll a). The European clams in Óbidos Lagoon recovered rapidly after their reproductive period, most likely owing to the availability of food. This study will help to improve sustainable management of this wild stock and is important for future aquaculture development of this species.