Susana Mendoza Elvira

Evaluación de la citotoxicidad de la aflatoxina y la fumonisina en células intestinales de porcino

Resumen La coexistencia de la aflatoxina (AFB) y la fumonisina (FB) es ampliamente conocida en muchas partes del mundo; sin embargo existen pocos estudios que describan el efecto sinergico de ambas micotoxinas in vivo o in vitro. El objetivo de este trabajo fue evaluar la citotoxicidad y el efecto de AFB y FB sobre la morfologia, la capacidad proliferativa celular y la sintesis de interleucina 8 (IL-8) en una linea celular de epitelio intestinal porcino (IPEC-1). Respecto a la morfologia celular, esta se vio afectada unicamente en las concentraciones mas altas de AFB (50 μM) y FB (500 μM). Sin embargo la proliferacion celular, el dano celular y la sintesis de IL-8 se vieron afectadas con la combinacion AFB/FB (1,3/3,7; 2/3,7 y 5/10 (M, respectivamente), al compararlas con el efecto individual de estas micotoxinas a las mismas concentraciones (p

Oral immunization against porcine pleuropneumonia using the cubic phase of monoolein and purified toxins of Actinobacillus pleuropneumoniae.

The main goal of this work was to obtain an orally administered immunogen that would protect against infections by Actinobacillus pleuropneumoniae. The Apx I, II and III toxins were obtained from the supernatants of cultures of serotypes 1 and 3 of A. pleuropneumoniae. The capacity of monoolein gel to trap and protect the Apx toxins, and the effect of their incorporation on the stability of the cubic phase were evaluated. The gel was capable of trapping a 400-μg/ml concentration of the antigen with no effects on its structure. Approximately 60% of the protein molecules were released from the gel within 4h. Four experimental groups were formed, each one with four pigs. All challenges were conducted in a nebulization chamber. Group A: Control (-) not vaccinated and not challenged; Group B: Control (+) not vaccinated but challenged; Group C: vaccinated twice intramuscularly with ToxCom (a commercial toxoid) at an interval of 15 days and then challenged; and Group D: vaccinated orally twice a week for 4 weeks with ToxOral (an oral toxoid) and challenged on day 28 of the experiment with a same dose of 2.0 × 10(4) UFC of A. pleuropneumoniae serotypes 1 and 3. The lesions found in group B covered 27.7-43.1% of the lungs; the pigs in group C had lesions over 12.3-28%; and those in group D over 15.4-32.3%. No lesions were found in the Group A pigs. A. pleuropneumoniae induced macroscopic lesions characteristic of infection by and lesions microscopic detected by histopathology. The etiologic agent was recovered from the infected lungs, tonsils and spleen. The serotypes identified were 1 and 3. An indirect ELISA test identified the antibodies against the Apx toxins in the serum of the animals immunized orally.

Identification and genotyping of porcine circovirus type II (PCV2) in Mexico

Porcine circovirus type 2 (PCV2), family Circoviridae, genus Circovirus infection in domestic pig has been associated with several pathological conditions being the most important of them the postweaning multisystemic wasting syndrome. Many studies have demonstrated the existence of three PCV2 genotypes (a, b, and c) and recently PCV3. Until now, these genotypes or subgenotypes have not been described in Mexico. We found genetic changes in ORF2 from nine strains of PCV2 obtained from samples of Jalisco, Veracruz, Estado de México, Hidalgo and Sonora states of Mexico. Our results shown the presence of two genotypes (PCV2a and PCV2b) as well as, the presence and differences between the reported subgenotypes. The subgenotype PCV2b (1A/1B, 1A) has a higher prevalence (87.5%) in comparison with PCV2a (2C) (12.5%).