Sushil Kumar Sharma

α-n-hydroxyamino acid derivatives

Abstract Reactions of organolithium reagents with glyoxylate derived oximes provided a direct route to α-N-hydroxyamino acids. The process required direct attachment of an ionizable group to the glyoxylate carbonyl to prevent competitive reactions. The procedure allowed for direct formation of the α-chiral center of the newly formed α-N-hydroxyamino acid derivative. Introduction of potential chiral auxiliaries on the oxime oxygen resulted in modest diastereoselection. In most instances, use of chiral glyoxylamides also gave low diastereoselectivity.

Reactions of organometallics with oximes. Synthesis of α-N-hydroxy amino acids

Abstract Reaction of organolithium reagents with glyoxylate and pyruvate derived oximes provides a direct route for the synthesis of unusual α-N-hydroxy amino acids.

The chemical synthesis of the chromogenic substrates H-D-VAL-L-LEU-L-LYS-p-Nitroanilide (S2251) and H-D-ILE-L-PRO-L-ARG-p-NITROANILIDE (S2288)

We describe detailed procedures for the chemical synthesis of two costly chromogenic substrates, H-D-Ile-L-Pro-L-Arg-p-nitroanilide (S2288) and H-D-Val-L-Leu-L-Lys-p-nitroanilide (S2251), which are widely employed for assay of tissue plasminogen activator and plasmin, respectively, as well as in inhibitor assays for these enzymes. Quantities of 5 grams or more of these reagents can be synthesized on a bench-top scale with routine equipment in a laboratory or facility with minimal experience in peptide chemistry. While some of the chemical reactions have been employed previously, improvements have been made therein in this manuscript, and these detailed descriptions are mainly provided to be of service to investigators who employ large quantities of these substrates in their work. The main strategic difference between our methodology and that employed previously is the coupling of the L-Lys- and L-Arg-p-nitroanilides to the previously synthesized, and otherwise completed peptides, which gives superior yields of final product and provides flexibility the chemical synthesis of substrates with other amino acids present.

Maleimide-assisted on-resin macrocyclization

Abstract The facile addition of a sulfhydryl group to a maleimide moeity has been exploited for onresin macrocyclization of peptides. This methodology can be easily extended to multiple synthesis of peptide/peptide-like molecules.