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Dive into the research topics where Susumu Uchiyama is active.

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Featured researches published by Susumu Uchiyama.


Communications Biology | 2018

Assembly of protein complexes restricts diffusion of Wnt3a proteins

Ritsuko Takada; Yusuke Mii; Elena Krayukhina; Yuusuke Maruyama; Kazuhiro Mio; Yoshikazu Sasaki; Takao Shinkawa; Chan-Gi Pack; Yasushi Sako; Chikara Sato; Susumu Uchiyama; Shinji Takada

Members of the Wnt protein family play roles in many aspects of embryogenesis and homeostasis. Despite their biological significance, characteristics of Wnt proteins still remain unclear, mainly due to their insolubility after the removal of serum. Here we examine Wnt proteins in serum-containing media by using analytical ultracentrifugation with a fluorescence detection system. This analysis reveals that Wnt3a assembles into high-molecular-weight complexes that become dissociable by interaction with the extracellular domain of the Frizzled8 receptor or secreted Wnt-binding protein sFRP2. Cross-linking and single-particle analyses of Wnt3a fractionated by gel filtration chromatography show the homo-trimer to be the smallest form of the assembled Wnt3a complexes. Fluorescence correlation spectroscopy and immunohistochemistry reveal that the assembly of Wnt3a complexes restricted their diffusion and signaling range in Xenopus laevis embryos. Thus, we propose that the Wnt diffusion range can be controlled by a balance between the assembly of Wnt complexes and their dissociation.Ritsuko Takada et al. show that Wnt3a assembles into high molecular weight complexes that restrict the diffusion of Wnt within Xenopus embryos. These results suggest that Wnt diffusion in cells is controlled by a balance between higher order complex assembly and dissociation by Wnt-binding proteins.


Journal of Biological Chemistry | 2000

Selected Mutations in a Mesophilic Cytochrome c Confer the Stability of a Thermophilic Counterpart

Jun Hasegawa; Susumu Uchiyama; Yuko Tanimoto; Masayuki Mizutani; Yuji Kobayashi; Yoshihiro Sambongi; Yasuo Igarashi


Archive | 2008

FEATURE QUANTITY SELECTION METHOD, FEATURE QUANTITY SELECTION APPARATUS, IMAGE CLASSIFICATION METHOD, IMAGE CLASSIFICATION APPARATUS, COMPUTER PROGRAM, AND RECORDING MEDIUM

Seiichiro Hasezawa; Natsumaro Kutsuna; Takumi Higaki; Satihiro Matsunaga; Susumu Uchiyama; Rika Maniwa; Arni eguna Gambe; Kiichi Fukui


Archive | 2008

Feature value selection method, feature value selection device, image classification method, image classification device, computer program, and recording medium

Seiichiro Hasezawa; Natsumaro Kutsuna; Takumi Higaki; Satihiro Matsunaga; Susumu Uchiyama; Rika Maniwa; Arni eguna Gambe; Kiichi Fukui


Archive | 2007

Isolation of Human and Plant Chromosomes as Nanomaterials

Susumu Uchiyama; Tomoyuki Doi; Kiichi Fukui


Archive | 2007

On-Chip Chromosome Sorter Using Electric and Magnetic Fields

Takahito Inoue; Yasuyuki Fujita; Susumu Uchiyama; Tomoyuki Doi; Kiichi Fukui; Hiroshi Yokoyama


The Japanese Biochemical Society/The Molecular Biology Society of Japan | 2017

Molecular basis of binding selectivity between axon guidance cue semaphorin and its receptor plexin

Tsubasa Tanaka; Makiko Neyazaki; Rika Oi; Emiko Mihara; Masanori Noda; Susumu Uchiyama; Junichi Takagi; Terukazu Nogi


日本生物工学会大会講演要旨集 | 2012

4Ep21 Effects of rotational speed on the hydrodynamic properties of antibodies measured by analytical ultracentrifugation sedimentation velocity

Elena Krayukhina; Susumu Uchiyama; Kiichi Fukui


生物物理 | 2011

2D1536 3α-hydroxysteroid dehydrogenaseの二量体形成能と酵素機能との相関(蛋白質_構造機能相関1,第49回日本生物物理学会年会)

Kotaro Hara; Masanori Noda; Susumu Uchiyama; Shigeru Ueda; Kiichi Fukui; Masayuki Oda


日本生物工学会大会講演要旨集 | 2011

2Ba07 Optimization of Agrobacterium mediated transformation efficiency in Jatropha curcas L.

Shota Yuasa; Naruemon Khemkladngoen; Joyce Cartagena; Susumu Uchiyama; Kiichi Fukui

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Tadayasu Ohkubo

Japan Advanced Institute of Science and Technology

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