Suyun Li

Association of Sleep Duration with the Morbidity and Mortality of Coronary Artery Disease: A Meta-analysis of Prospective Studies

PURPOSE Insufficient and excessive sleep duration have become increasingly common in modern society. Published literatures report controversial results about the association of sleep duration with coronary artery disease (CAD). METHODS A comprehensive search was performed to identify related prospective studies providing quantitative estimates between sleep duration and CAD. Meta-analysis was applied to calculate the combined relative risks (RRs) with 95% confidence intervals (CI) for sleep with morbidity and mortality of CAD. The risk of bias was assessed by the Egger regression asymmetry test. RESULTS Fifteen studies conformed to the criteria. Compared with normal sleep duration, the pooled RRs (95%CI) of short sleep duration were 1.10(1.04-1.17) and 1.25(1.06-1.47) for the morbidity and mortality of CAD, and the pooled RRs (95%CI) of long sleep duration were 1.03(0.92-1.16) and 1.26(1.11-1.42) for the morbidity and mortality of CAD, respectively. The effect of short and long sleep duration on mortality of CAD were always significantly greater than the morbidity of CAD. CONCLUSIONS Short sleep duration was associated with higher morbidity of CAD, and short sleepers and long sleepers had higher risk for CAD mortality. Keeping normal sleep duration is an appropriate recommendation to prevent and control CAD.

Association between variants in nicotinic acetylcholine receptor genes and smoking cessation in a Chinese rural population

BACKGROUND AND OBJECTIVES Genetic studies have revealed a significant association between variants in nicotinic acetylcholine receptor (CHRN) genes and smoking cessation, but the results are inconsistent. In this study, we aimed to examine the relationship between single nucleotide polymorphisms (SNPs) in seven CHRN genes and smoking cessation in a Chinese rural population. METHODS Participants were recruited from 17 villages of 3 counties in Shandong, China. DNA was extracted from the blood samples. Thirty-two SNPs in seven CHRN genes were genotyped. Logistic regression was used to explore the relationship between single SNP and smoking cessation. Pearsons χ(2) test was performed to test the association between haplotype and smoking cessation. RESULTS Rs578776 (in CHRNA3), rs660652 (in CHRNA3), and rs588765 (in CHRNA5) were significantly related to smoking cessation. Two haplotypes were associated with smoking cessation. CONCLUSIONS This study confirmed the association between CHRN genes and smoking cessation in the Chinese rural population. SCIENTIFIC SIGNIFICANCE Our findings provide confirmatory support to the role of CHRN genes to the etiology of smoking cessation in the Chinese rural population. (Am J Addict 2016;25:297-300).

Association of the G1057D polymorphism in insulin receptor substrate 2 gene with type 2 diabetes mellitus: a meta-analysis

OBJECTIVES The G1057D polymorphism in insulin receptor substrate (IRS)-2 gene is associated with type 2 diabetes mellitus (T2DM) risk, but results in published literatures are controversial. In addition, the effect of obesity as a modifier on this association is also inconsistent. Thus, this meta-analysis was performed to assess the above-mentioned association. METHODS A comprehensive search was performed to identify case-control or cohort studies (from 1990 to 2014) of the aforementioned association. The I(2) statistic was used to examine between-study heterogeneity. Fixed or random effect model was selected based on heterogeneity test among studies. Publication bias was estimated using modified Eggers regression test. RESULTS Nine articles with ten studies were included. After excluding studies deviated from Hardy-Weinberg equilibrium (HWE) in controls, results showed a significant association of D allele with reduced T2DM risk in dominant (OR = 0.825, 95% CI: 0.705-0.965) and codominant (OR = 0.857, 95% CI: 0.763-0.964) models, but no significant association in recessive (OR = 0.806, 95% CI: 0.628-1.035) model. For studies stratified by obesity, after excluding studies deviated from HWE in controls, no significant association of D allele with T2DM risk was found in three inherited models in obese group; however, a significant protective effect of D allele was observed in dominant (OR = 0.714, 95% CI: 0.533-0.958), recessive (OR = 0.438, 95% CI: 0.253-0.760) and codominant (OR = 0.706, 95% CI: 0.565-0.883) models in non-obese group. CONCLUSIONS This meta-analysis suggested that D allele of G1057D polymorphism have a significant effect on reduced risk of T2DM, and obesity is a modifier of this association. This result needs to be confirmed by further studies.

The association of dopamine pathway gene score, nicotine dependence and smoking cessation in a rural male population of Shandong, China

BACKGROUND AND OBJECTIVES Smoking and smoking cessation are both psychological and physiological traits. We aimed to investigate the interaction between dopamine pathway gene scores and nicotine dependence on smoking cessation in a rural Chinese male population. METHODS Participants were recruited from 17 villages in Shandong, China. DNA was extracted from blood sample of 819 participants. 25 single nucleotide polymorphisms (SNPs) in 8 dopamine (DA) pathway genes were genotyped. Weighted gene score of each gene is used to analyze the whole gene effect. Logistic regression was used to calculate odds ratios (OR) and multivariate-adjusted OR of each gene score for smoking cessation. Multiplicative model interaction was assessed through a cross-product interaction term of gene score by nicotine dependence in a multivariate logistic regression model. RESULTS After adjusting for age, occupation, education, marital status, self-rating anxiety score, and disease status, we observed significant negative associations of catechol-O-methyltransferase (COMT), dopamine receptor D2 (DRD2) gene score and smoking cessation, as well as significant positive associations between ankyrin repeat and kinase domain containing 1 (ANKK1), dopamine transporter (SLC6A3), dopamine receptor D4 (DRD4) gene score and smoking cessation. A significant multiplicative model interaction between nicotine dependence and the SLC6A3 gene score on smoking cessation was also observed (p = .03). CONCLUSIONS AND SCIENTIFIC SIGNIFICANCE There is a significant multiplicative model interaction of SLC6A3 gene score and nicotine dependence on smoking cessation. This finding could help to identify smokers who may be at high risk of relapse, and thus to develop more professional and personalized smoking cessation treatment. (Am J Addict 2016;25:493-498).

Assessment of successful smoking cessation by psychological factors using the Bayesian network approach.

The association between psychological factors and smoking cessation is complicated and inconsistent in published researches, and the joint effect of psychological factors on smoking cessation is unclear. This study explored how psychological factors jointly affect the success of smoking cessation using a Bayesian network approach. A community-based case control study was designed with 642 adult male successful smoking quitters as the cases, and 700 adult male failed smoking quitters as the controls. General self-efficacy (GSE), trait coping style (positive-trait coping style (PTCS) and negative-trait coping style (NTCS)) and self-rating anxiety (SA) were evaluated by GSE Scale, Trait Coping Style Questionnaire and SA Scale, respectively. Bayesian network was applied to evaluate the relationship between psychological factors and successful smoking cessation. The local conditional probability table of smoking cessation indicated that different joint conditions of psychological factors led to different outcomes for smoking cessation. Among smokers with high PTCS, high NTCS and low SA, only 36.40% successfully quitted smoking. However, among smokers with low pack-years of smoking, high GSE, high PTCS and high SA, 63.64% successfully quitted smoking. Our study indicates psychological factors jointly influence smoking cessation outcome. According to different joint situations, different solutions should be developed to control tobacco in practical intervention.

Associations of cytochrome P450 oxidoreductase genetic polymorphisms with smoking cessation in a Chinese population

Recently, a single nucleotide polymorphism (SNP) A503V (rs1057868) in cytochrome P450 oxidoreductase (POR) gene was reported to influence nicotine metabolism. Considering the importance of nicotine metabolism to smoking cessation, the aim of this study was to investigate the association between POR gene polymorphisms and smoking cessation in a Chinese population. A case–control study was conducted with 363 successful smoking quitters as the cases, and 345 failed smoking quitters as the controls. Eight tagSNPs which cover the entire gene and four functional SNPs were selected and genotyped. Logistic regression was used to explore the relationship between POR SNPs and smoking cessation in codominant, additive, dominant and recessive models. After adjustment for potential confounders, multiple logistic regression analysis indicated that POR rs3823884 and rs3898649 were associated with increased possibility of smoking cessation. Meanwhile, POR rs17685 and rs239953 were shown to have negative effect on successful smoking cessation. No significant differences in the distribution of haplotypes between cases and controls were detected. In conclusion, this study reveals that four SNPs in the POR gene (rs3823884, rs3898649, rs239953 and rs17685) may affect the susceptibility of smoking cessation in a Chinese Han population.

Association of serotonergic pathway genes with smoking cessation in a Chinese rural male population

INTRODUCTION Previous studies have found serotonergic pathway genes have inhibitory effects on dopamine system which may influence smoking addiction. This study examined the associations of serotonergic pathway genes (serotonergic receptor genes, solute carrier family 6 member4 and tryptophan hydroxylase gene) with smoking cessation. MATERIALS AND METHODS Male current and former smokers (n=819) were recruited from 17 villages of three counties in Shandong province, China. DNA was extracted from the blood samples. Eleven single nucleotide polymorphisms (SNPs) in serotonergic pathway genes were genotyped. Multiple logistic regression was used to assess associations between SNPs and smoking cessation. Pearsons χ2 test was performed to explore associations of haplotypes with smoking cessation. Multiple logistic regression was used to detect the interaction between SNPs on smoking cessation. RESULTS In multiple logistic regression, rs1042173 of Solute carrier family 6 member 4 was significantly related to smoking cessation in additive and dominant model (p=0.03 and 0.02, respectively). Rs4570625 of tryptophan hydroxylase 2 was significantly associated with smoking cessation in dominant model (p=0.03). Nine significant interactions were detected between SNPs in serotonergic pathway genes. CONCLUSIONS The present study reveals that serotonergic pathway genes were significantly related to smoking cessation. Future research should expand upon these findings to confirm them.

The association of environmental, individual factors, and dopamine pathway gene variation with smoking cessation

Abstract This study aimed to examine whether dopamine (DA) pathway gene variation were associated with smoking cessation, and compare the relative importance of infulence factors on smoking cessation. Participants were recruited from 17 villages of Shandong Province, China. Twenty-five single nucleotide polymorphisms in 8 DA pathway genes were genotyped. Weighted gene score of each gene was used to analyze the whole gene effect. Logistic regression was used to calculate odds ratios (OR) of the total gene score for smoking cessation. Dominance analysis was employed to compare the relative importance of individual, heaviness of smoking, psychological and genetic factors on smoking cessation. 415 successful spontaneous smoking quitters served as the cases, and 404 unsuccessful quitters served as the controls. A significant negative association of total DA pathway gene score and smoking cessation was observed (p < 0.001, OR: 0.25, 95% CI 0.16–0.38). Dominance analysis showed that the most important predictor for smoking cessation was heaviness of smoking score (42%), following by individual (40%), genetic (10%) and psychological score (8%). In conclusion, although the DA pathway gene variation was significantly associated with successful smoking cessation, heaviness of smoking and individual factors had bigger effect than genetic factors on smoking cessation.

Relative contribution of genetic, individual, and self-efficacy factors to smoking cessation in a Chinese rural population

BACKGROUND AND OBJECTIVES Nicotine dependence is influenced by genetic, individual, and psychological factors. We aimed to examine whether nicotinic acetylcholine receptor genes (CHRN) were associated with smoking cessation (SC) using genetic risk score and compare the relative contribution of genetic, individual and self-efficacy factors to SC. METHODS Eight hundred and nineteen male smokers (mean age: 59.62) were recruited from 17 villages of three counties in Shandong province, China. Thirty-two single nucleotide polymorphisms (SNPs) in seven CHRN genes were genotyped. Logistic regression was used to explore the relationship between genetic risk score and SC. Dominance analysis was performed to compare the relative contribution of genetic, individual, and self-efficacy factors on SC. RESULTS CHRNA3 genetic risk score was associated with SC. Dominance analysis showed that individual factor was the most important predictor for SC, followed by genetic and self-efficacy factors. CONCLUSIONS CHRNA3 was associated with successful SC. Individual factor had more contribution than genetic factor to SC. SCIENTIFIC SIGNIFICANCE Our findings provide support to the role of CHRN genes in the etiology of smoking cessation using genetic risk score. Individual factor should be particularly valued in smoking control intervention. (Am J Addict 2017;26:161-166).