Suzanne P. Thompson

DENITRIFICATION IN A CONSTRUCTED WETLAND RECEIVING AGRICULTURAL RUNOFF

Constructed wetlands are recognized as a means to improve water quality through nitrogen (N) removal. Water-quality concerns in the N-sensitive Neuse River Estuary, North Carolina, USA, have necessitated enactment of a 30% reduction, in nitrogen (N) loading accompanied by an N loading cap. Open Grounds Farm (OGF) is an 18,220-ha row-crop farm located in the lower Neuse River Estuary. In 1999, a wetland was constructed to remove nutrients (N and Phosphorus), sediment, and pathogens in surface water draining from a 971-ha area of OGF. The wetland site is 5.1 ha of alternating segments of emergent marsh and open water. Nitrogen removal from the wetland via denitrification was measured monthly by analysis of dissolved nitrogen, oxygen, and argon in laboratory incubated sediment chambers using a Membrane Inlet Mass Spectrometer (MIMS). Nitrate concentration appeared to be the primary variable controlling denitrification rates. Spatial and temporal variability in rates of denitrification were investigated, including pre- and post- N loading events. Following rainfall, there was a 400% increase in denitrification rates in response to increased inorganic N loading. Nutrient loads entering and leaving the wetland were determined from nutrient analysis (twice monthly), intensive precipitation event sampling, and continuous flow measurements at the entrance and exit of the, wetland. Results indicated that the wetland received variable N loading (1-1,720 kg N per month) and had variable N removal via denitrification (8-81 kg N per month). Denitrification was an important mechanism for N removal.

Seasonal Patterns of Nitrification and Denitrification in a Natural and a Restored Salt Marsh

Seasonal patterns of microbially-mediated nitrogen cycling via the nitrification-denitrification pathway were compared between a natural and a restored salt marsh. Sedimentary denitrification rates, measured with a modification of the acetylene block technique, were approximately 44 times greater in the natural marsh relative to an adjacent transplanted marsh. Nitrification rates were similar at both sites. The difference in denitrification rates was attributed to oxygen inhibition at low tide and tidal flushing of porewater nutrients at high tide in the coarse sediments of the restored marsh. Denitrification was positively correlated with nitrification throughout the year in the natural marsh with a seasonal fall peak in denitrification corresponding to a maximum in porewater ammonia concentration. A weak correlation existed between the two processes in the restored marsh, where nitrification rates exceeded denitrification rates by a factor of 20. Transplanted marsh denitrification rates exhibited a spring peak, corresponding to elevated porewater ammonia concentrations. Our findings demonstrate functional differences in microbial nitrogen dynamics of a young (0–3 yr) restored marsh relative to a mature (>50 yr) salt-marsh system. *** DIRECT SUPPORT *** A01BY070 00008

Denitrification Rates Measured Along a Salinity Gradient in the Eutrophic Neuse River Estuary, North Carolina, USA

Denitrification rates along a salinity gradient in the eutrophic Neuse River Estuary, North Carolina, were quantified using membrane inlet mass spectrometry (MIMS) within short-term batch incubations. Denitrification rates within the system were highly variable, ranging from 0 to 275 μmol N m−2 h−1. Intrasite variability increased with salinity, but no significant differences were observed across the salinity gradient. Denitrification rates were positively correlated with sediment oxygen demand at the upstream sampling site where sediment organic carbon levels were lowest. This relationship was not observed in the more saline sampling sites. Denitrification rates were highest during winter. On an annual basis, denitrification accounted for 26% of the dissolved inorganic nitrogen and 12% of the total nitrogen supplied to the system.

Denitrification of Nitrogen Released from Senescing Algal Biomass in Coastal Agricultural Headwater Streams

Assimilation of inorganic N by photoautotrophs has positive impacts on nutrient retention; however this retention is only temporary. As the biomass senesces, organic and inorganic forms of N are released back to the stream where they can be further transformed (i.e., nitrification, denitrification) or exported downstream. The purpose of this study was to assess the fate of the remineralized N, particularly the potential for removal by denitrification. Experiments were conducted on intact sediment cores from streams in an agricultural watershed. Cores were amended with varying ages of algal leachate and denitrification rates were measured with a membrane inlet mass spectrometer. Results of this study demonstrated that senescing algal biomass stimulated denitrification rates and provided a source of N and labile C to denitrifiers. Regardless of leachate age, addition of leachate to intact cores revealed a net loss of dissolved inorganic N from the water column. Denitrification rates were most strongly related to concentrations of dissolved and particulate C in the overlying water and secondarily to C quality (molar C to N ratio of total dissolved C and N) and NO(3)(-) flux. Using a mass balance approach, the proportion of N from senescing algal biomass that was denitrified accounted for as much as 10% of the total dissolved nitrogen (TDN) and up to 100% of the NO(3)(-) during a 3-h experiment. These results suggest an important link between instream algal uptake and eventual denitrification thereby providing a pathway for permanent removal of watershed-derived N from the stream ecosystem.

Coastal stormwater wet pond sediment nitrogen dynamics

Wet ponds are a common type of stormwater control measure (SCM) in coastal areas of the southeastern US, but their internal nitrogen dynamics have not been extensively studied. Using flow-through intact sediment core incubations, net sediment N2 fluxes before and after a nitrate addition from five wet ponds spanning a range of ages (3.25-10years old) were quantified through membrane inlet mass spectrometry during early summer. Multiple locations within a single wet pond (6.16years old) were also sampled during ambient conditions in late summer to determine the combined effects of depth, vegetation, and flow path position on net N2 fluxes at the sediment-water interface. All pond sediments had considerable rates of net nitrogen fixation during ambient conditions, and net N2 fluxes during nitrate-enriched conditions were significantly correlated with pond age. Following a nitrate addition to simulate storm conditions, younger pond sediments shifted towards net denitrification, but older ponds exhibited even higher rates of net nitrogen fixation. The pond forebay had significantly higher rates of net nitrogen fixation compared to the main basin, and rates throughout the pond were an order of magnitude higher than the early summer experiment. These results identify less than optimal nitrogen processing in this common SCM, however, data presented here suggest that water column mixing and pond sediment excavation could improve the capacity of wet ponds to enhance water quality by permanently removing nitrogen.

Nitrogen cycling processes within stormwater control measures: A review and call for research

Stormwater control measures (SCMs) have the potential to mitigate negative effects of watershed development on hydrology and water quality. Stormwater regulations and scientific literature have assumed that SCMs are important sites for denitrification, the permanent removal of nitrogen, but this assumption has been informed mainly by short-term loading studies and measurements of potential rates of nitrogen cycling. Recent research concluded that SCM nitrogen removal can be dominated by plant and soil assimilation rather than by denitrification, and rates of nitrogen fixation can exceed rates of denitrification in SCM sediments, resulting in a net addition of nitrogen. Nitrogen cycling measurements from other human-impacted aquatic habitats have presented similar results, additionally suggesting that dissimilatory nitrate reduction to ammonium (DNRA) and algal uptake could be important processes for recycling nitrogen in SCMs. Future research should directly measure a suite of nitrogen cycling processes in SCMs and reveal controlling mechanisms of individual rate processes. There is ample opportunity for research on SCM nitrogen cycling, including investigations of seasonal variation, differences between climatic regions, and trade-offs between nitrogen removal and phosphorus removal. Understanding nitrogen dynamics within SCMs will inform more efficient SCM design and management that promotes denitrification to help mitigate negative effects of urban stormwater on downstream ecosystems.