Svätoslav Hluchý

The Influence of Ultra-Violet Radiation on Chicken Hatching

Abstract The influence of UV-B radiation on embryonic development of chickens Hampshire breed was investigated. The set eggs with the average weight of 60.0 ± 0.5 g were divided into six groups. The chickens in the control group C were hatched in the darkness. The chicken embryos in experimental groups were, during their incubation, influenced by UV light: in E1 (1 h a day), in E2 (2 h a day), in E3 (3 h a day), in E4 (4 h a day), and in E5 (5 h a day). After the experiment, we can state that UV radiation appealing on chickens embryos of shorter time (1–2 h) was reflected in decreasing embryonic mortality in experimental group E1 (1.27 ± 0.14%), the embryonic development was accelerated and the weight of hatched chickens was increased in group E2 (492.43 ± 5.02 h and 47.83 ± 2.62 g, respectively). The negative influence of UV radiation was reflected while it is longer appealing on chickens embryos (for 3–5 h), mainly by increased embryonic mortality in groups E3 (10.27 ± 1.65%), E4 (58.09 ± 3.12%), and E5 (100.00 ± 0.00%). The results obtained are highly significant (p < 0.001) in comparison with a control group C, as well as, with the experimental groups E1 and E2.

Distribution of diazinon and selenium in various tissues after single and common intraperitoneal administration

The aim of this study was to evaluate the distribution of diazinon and selenium in various tissues of laboratory rats after single and common intraperitoneal administration. Rats in the age of 135 days were randomly divided into 4 groups. Each group consisted of 10 males. Animals in the first group were administrated with diazinon 20 mg kg body weight intraperitoneally. Animals in the second group were administrated with selenium (Na2SeO3) 2 mg Se kg –1 body weight intraperitoneally in physiological solution. Animals in the third group were given a mixture of diazinon 20 mg kg body weight and selenium 2 mg Se kg body weight intraperitoneally in physiological solution. The fourth group served as a control group and was administrated only with the physiological solution. 24 hours after the administration of tested substances, animals were sacrificed and samples of livers, kidneys, muscles and fat tissue were taken during the autopsy. The amount of diazinon in tissues was determined using gas chromatography with mass spectrometry. The amount of selenium was determined using atomic absorption spectrometry. We detected significant increase of selenium amount in livers, kidneys and muscles after single selenium administration and also after common administration of both substances. On the other hand we detected significant decrease of selenium amount in muscles and fat tissue after single diazinon administration. We also observed slight accumulation of diazinon amount in samples of kidneys and muscles and significant increase of diazinon in fat tissue after single diazinon administration and also after common administration of both substances.