Syed Umer Jan

Effect of cisplatin on glutathione redox status in isolated plasma and cytosolic fraction

Cisplatin has been used therapeutically in the treatment of malignant tumors. Meanwhile, the major limitations associated with cisplatin are its side effects in the form of nephrotoxicity, neurotoxicity, emetogensis and emerging resistance. Most of these problems are due to its adverse effects on the body’s endogenous cytoprotective molecules like glutathione (GSH). In current study, the effect of glutathione on the improvement of cisplatin therapy along with control on its growing problem of resistance was emphasized. The effect of cisplatin on the chemical and metabolic status of glutathione was evaluated in human venous blood after its separation in to plasma and cellular fraction using ultra violet (UV)-visible spectrophotometer. The glutathione in isolated plasma and cellular fractions of the blood was exposed to different concentrations of cisplatin. It was found that there was a gradual depletion in the concentration of reduced glutathione. Similarly, time-dependent effect of cisplatin was also evaluated on the status of glutathione, in which positive correlation was found between exposure of glutathione to the given concentrations of cisplatin and the depletion of reduced glutathione as the time passed from 0 to 5 h. This depletion in the concentration of reduced GSH is either due to formation of Pt-SG complex or due to the conversion of this multifunctional molecule (glutathione) to its physiologically inactive disulfide form (GSSG). This study was carried out in vitro, which in principle depicts a model of in vivo reaction. This decrease in blood GSH levels after cisplatin treatment will result in decreased antioxidant capacity of the blood, which in turn will result in numerous pathological conditions.

Oxidation of glutathione (GSH) in blood plasma due to oxidative stressors: A case study of silver

1 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Gomal University, D. I. Khan, Pakistan. 2 Department of Pharmaceutics, Faculty of Pharmacy, Gomal University, D. I. Khan, Pakistan. 3 Faculty of Pharmacy and Alternative Medicine, the Islamia University of Bahawalpur, Pakistan. 4 Department of Pharmacy, University of Baluchistan, Quetta, Pakistan. 5 Gomal Center of Biochemistry and Biotechnology, Gomal University, D. I. Khan KPK, Pakistan.

STUDY OF THE CHEMICAL AND METABOLIC CHANGES IN PLASMA GLUTATHIONE (GSH) OF HUMAN BLOOD AFTER LITHIUM INTRODUCTION

Lithium remains a mainstay in the acute and prophylactic treatment of bipolar affective disorder. It is used in the augmentation of antidepressant treatment and, less frequently, in the augmentation of antipsychotic treatment of schizophrenia. It is reported to have specific anti-suicidal effects. Systematic reviews by the Cochrane collaboration and others have examined the evidence base or its use in these contexts. Thus it is interesting to study the effect of Lithium on the Glutathione. The effect of Lithium on the chemical status of the glutathione in plasma has been studied using Ellman’s method. The effect of Lithium on the chemical status of glutathione was determined in plasma for concentration and time dependent effects. There was found a drastic effect on decreasing the concentration of glutathione in plasma as the concentration is increased and time has passed. The decrease in the Glutathione level was concentration and time of interaction dependent, probably due to oxidation of GSH to corresponding disulphide (GSSG).In this paper the effect of Lithium metal on thiol /GSH level was discussed in vitro, which in principal may present a model of in vivo reaction.

CONCENTRATION AND TIME DEPENDENT EFFECT OF ALUMINIUM METAL ON GLUTATHIONE LEVEL IN PLASMA OF HUMAN BLOOD

Aluminium is an important metalloelement and has many medicinal uses like use of aluminum as an antacid. Thus it is interesting to study the effect of Aluminium on the glutathione (GSH) in vivo conditions. The concentration and time dependent effect of Aluminium on glutathione level in plasma was studied by using Ellman’s method. A drastic effect on decreasing the concentration of glutathione level in plasma was found by increasing the concentration of Aluminium sulphate over the time. The reason could probably be due to oxidation of GSH to corresponding disulphide (GSSG). In this paper the effect of Aluminium metal on thiol/GSH level was discussed in vitro, which in principal may present a model of in vivo reaction