Sylvie Baudino
French Institute of Health and Medical Research
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Publication
Featured researches published by Sylvie Baudino.
Molecular Breeding | 2001
Veronique Gruber; Patrick P. Berna; Thierry Arnaud; Philippe Bournat; Christèle Clément; Dominique Mison; Béatrice Olagnier; Laurence Philippe; Manfred Theisen; Sylvie Baudino; Claude Benicourt; Claire Cudrey; Carole Bloës; Nathalie Duchateau; Sylvie Dufour; Catherine Gueguen; Séverine Jacquet; Catherine Ollivo; Christine Poncetta; Nathalie Zorn; Dolores Ludevid; Alain Van Dorsselaer; Robert Verger; Annette Marian Doherty; Bertrand Merot; Charles Danzin
A recombinant dog gastric lipase with therapeutic potential for the treatment of exocrine pancreatic insufficiency was expressed in transgenic tobacco plants. We targeted the protein using two different signal sequences for either vacuolar retention or secretion. In both cases, an active glycosylated recombinant protein was obtained. The recombinant enzymes and the native enzyme displayed similar properties including acid resistance and acidic optimum pH. The proteolytic maturation and the specific activity of the recombinant proteins, however, were found to be dependent on subcellular compartmentalization. Expression levels of recombinant dog gastric lipase were about 5% and 7% of acid extractable plant proteins for vacuolar retention and secretion respectively. This expression system already has allowed the production of tens of grams of purified lipase through open-field culture of transgenic tobacco plants.
Nature | 1997
Wilfrid Dieryck; J. Pagnier; Claude Poyart; Michael C. Marden; Véronique Gruber; Philippe Bournat; Sylvie Baudino; Bertrand Merot
Archive | 1993
Philippe Lenee; Veronique Gruber; Sylvie Baudino; John Mason; David Comeau; Mohamad Ali Rezaian; Ian B. Dry; Justin E. Rigden
Archive | 1996
Bertrand Merot; Wilfrid Dieryck; Philippe Lenee; Michael C. Marden; Véronique Gruber; Renee-Josee Pagnier; Sylvie Baudino; Claude Poyart
Archive | 1996
Philippe Lenee; Veronique Gruber; Sylvie Baudino; Bertrand Merot; Claude Benicourt; Claire Cudrey
Archive | 2001
Bertrand Merot; Wilfrid Dieryck; Philippe Lenee; Michael C. Marden; Véronique Gruber; Renee-Josee Pagnier; Sylvie Baudino; Claude Poyart
Archive | 1996
Bertrand Merot; Sylvie Baudino; Veronique Gruber; Philippe Bournat; Philippe Lenee; Michel Riviere; Jean-Christophe Audonnet
Archive | 2002
Philippe Lenee; Veronique Gruber; Sylvie Baudino; Bertrand Merot; Claude Benicourt; Claire Cudrey
Archive | 1996
Sylvie Baudino; Wilfrid Dieryck; Veronique Gruber; Philippe Lenee; Michael C. Marden; Bertrand Merot; Renee-Josee Pagnier; Claude Poyart
Transfusion Clinique Et Biologique | 1998
J. Pagnier; Michael C. Marden; Claude Poyart; Véronique Gruber; Pascal Burnat; Sylvie Baudino; Manfred Theisen; Bertrand Merot
Collaboration
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Commonwealth Scientific and Industrial Research Organisation
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Commonwealth Scientific and Industrial Research Organisation
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