Sylwia Grabska

3D composites based on the blends of chitosan and collagen with the addition of hyaluronic acid

3D porous composites based on blends of chitosan, collagen and hyaluronic acid were obtained through the lyophilization process. Mechanical properties, swelling behavior and thermal stability of the blends were studied. Moreover, SEM images were taken and the structure of the blends was studied. Biological properties of the materials obtained were investigated by analyzing of proliferation rate of fibroblast cells incubated with biomaterial extract using MTT assay (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide). The results showed that the properties of 3D composites based on the blends of chitosan and collagen were altered after the addition 1%, 2% and 5% of hyaluronic acid. Mechanical properties and thermal stability of chitosan/collagen blends were improved in the presence of hyaluronic acid in the composite. New 3D materials based on the blends of chitosan, collagen and hyaluronic acid were non-toxic and did not significantly affect cell morphology.

Surface and thermal properties of collagen/hyaluronic acid blends containing chitosan

The structure and surface properties of binary and ternary blends containing collagen (Coll), hyaluronic acid (HA) and chitosan (Ch) were investigated by contact angle measurements, thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and atomic force microscopy (AFM). Thin films of Coll/HA and Coll/HA/Ch blends have been formed by casting methods from aqueous acid solutions. The surface roughness, hydrophobic/hydrophilic character and thermal stability of Coll/HA were changed after addition of chitosan. Thermal stability of binary blends increase upon the addition of chitosan. The results of contact angle and the surface free energy revealed that hyaluronic acid films are more polar than collagen and chitosan films. The surface energy and its polar and dispersive components of binary and ternary blends were calculated and more hydrophilic films were produced by the addition of HA and chitosan, also resulting in more thermally stabile materials. These results demonstrate that collagen interacts with hyaluronic acid and chitosan changing the surface properties of polymer films.

Physico-chemical properties of three-component mixtures based on chitosan, hyaluronic acid and collagen

ABSTRACT The aim of this work was preparation of biopolymer materials based on blends of chitosan, hyaluronic acid and collagen. The properties of blend films have been investigated by contact angle measurements and atomic force microscopy. The contact angle measurements showed that chitosan films are more polar after addition of hyaluronic acid and collagen. The AFM results showed that the addition of chitosan to hyaluronic acid led to the increase of surface roughness. Moreover, miscibility studies of biopolymeric blends were also done using the viscometric method. Viscometric studies indicated that two-component and three-component blends are partially miscible.

Structure and Interactions in Chitosan Composites

In the present study, the results of Fourier transform infrared spectroscopy (FTIR) and atomic force microscopy (AFM) studies of chitosan (Ch) with montmorillonite (MMT) or nanoclay are presented. MMT samples have been used without modification and after nanoclay surface modification. Composites of chitosan with montmorillonite samples were obtained by drop casting suspensions of montmorillonite (3% relative to chitosan) and polymer (2wt%) in 1% acetic acid. The FTIR spectra of film composites in the amide I, amide II bands and the hydroxyl stretching bands of chitosan have been analyzed. The homogeneity and morphology of chitosan composites were ascertained from tapping-mode atomic force microscopy. The obtained results suggested that there were interactions between chitosan and montmorillonite. The microstructure obtained by AFM microscopy for chitosan and its composites showed that particles are relatively well dispersed in the chitosan matrix.

Preparation and characterization of silk fibroin/collagen sponge modified by chemical cross-linking

ABSTRACT 3D scaffolds made of blend of silk fibroin and collagen were obtained by lyophilisation process and cross-linking with EDC/NHS. The structure of the scaffolds was studied by ATR-FTIR spectroscopy. Morphological properties were analyzed by SEM. Porosity for cross-linked samples were measured. The mechanical properties of samples were tested in dry and wet conditions and compared with non-modified samples. The results showed that the blend of silk fibroin and collagen after cross-linking with EDC/NHS and lyophilisation has porous structure. Mechanical properties were improved by cross-linking with EDC/NHS. Moreover, the cross-linked scaffolds have got better stability in water than non cross-linked ones.

Preparation and characterization of collagen/chitosan/hyaluronic acid thin films for application in hair care cosmetics

Abstract In this study thin films based on a blend of collagen, chitosan and hyaluronic acids were prepared and their surface and mechanical properties were studied. The structure of the films was studied using FTIR spectroscopy, contact angle measurement and AFM images. Swelling and mechanical analyses were also performed. The hair protection possibility of collagen/chitosan/hyaluronic was studied using SEM microscopy and the mechanical testing of hair coated by the blends. It was found that the addition of hyaluronic acid to a collagen/chitosan blend improves the mechanical resistance of biopolymeric films. Samples with the addition of hyaluronic acid were more stable in aqueous conditions and provided higher roughness of surface.

CHARACTERISATION OF CHITOSAN/HYALURONIC ACID BLEND FILMS MODIFIED BY COLLAGEN

In the present study, thin films based on the blends of chitosan (Ch) and hyaluronic acid (HA) with and without collagen (Coll) were characterised using tensile tests, attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and thermogravimetric analysis (TGA). Ch/HA and Ch/HA/Coll polymer blends were prepared using the solvent evaporation technique. The interactions between polymer components in the binary and ternary blends were studied by FTIR analysis. Mechanical properties were studied and compared with those of simple polymer films. These results show that the addition of collagen into Ch/HA blends led to the increase of tensile strength and Young modulus. Moreover, it was found that the thermal stability of the Ch/HA binary blend significantly increased upon the addition of collagen.

THE INFLUENCE OF THE TYPE SOLVENT ON THE STRUCTURE OF CHITOSAN BLENDS WITH HYALURONIC ACID

The influence of the type solvent on the structure of chitosan, hyaluronic acid and their blend films was investigated. Aqueous acetic acid, hydrochloric acid, sodium chloride and aqueous acetic acid/NaCl were used as solvents for chitosan, hyaluronic acid and Ch/HA solution blends. Ch, HA and their blend films were prepared by casting technique. The homogeneity and morphology of chitosan blends were ascertained from the tapping-mode atomic force microscopy (AFM) and scanning electron microscopy (SEM). The changes of topography images are considered by determining the root mean square (RMS, Rq) deviation in the image data. The surface roughness of chitosan, hyaluronic acid and Ch/HA blended films was altered by mixing. The structure of chitosan blends with hyaluronic acid depends on the blend composition and on the solvent used for preparing the blend.

Polymer films based on silk fibroin and collagen - the physico-chemical properties

ABSTRACT Thin films based on the blend of collagen and silk fibroin were prepared. Biopolymers were dissolved separately in an aqueous solution, mixed together, and after solvent evaporation films were obtained. The surface properties of films have been studied by the contact angle measurements and atomic force microscopy. The mechanical properties such as Young modulus, tensile strength and elongation at break were measured. The results suggest that modification of properties of biopolymeric materials is a consequence of the strong interaction between the polymeric components. The structure and surface properties of two-component blends depend on the mixture composition.