T.D. Lester

Influence of toxic endophyte-infected fescue on sperm characteristics and endocrine factors of yearling Brahman-influenced bulls

Sixteen (mean age = 1.1 +/- 0.1 yr; mean BW = 478 +/- 34 kg) Brahman-influenced bulls were used to determine the influence of fescue type on sperm characteristics and serum concentrations of prolactin, cortisol, and testosterone. Bulls were blocked by BW, scrotal circumference (SC), and pregrazing sperm characteristics and randomly assigned to graze toxic endophyte-infected (EI; 4 bulls/pasture; 2 pastures) or novel endophyte-infected (NE; 4 bulls/pasture; 2 pastures) tall fescue for 121 d. Semen was collected by electroejaculation, and SC was measured and blood samples collected monthly. Sperm were evaluated for motility and morphology with an integrated visual optical system. Overall mean concentration of prolactin was decreased more (P < 0.01) in EI bulls than NE bulls from May to August. Scrotal circumference was not affected by fescue type (P = 0.58); overall SC averaged 36.7 +/- 2.3 cm. Percentage of live sperm was not different (P = 0.24) between NE bulls (80%) than EI bulls (67%) in July and August. Bulls grazing NE fescue had more (P < 0.06) motile sperm than EI bulls in July and August. Percentages of progressive (57 vs. 38%, NE and EI, respectively; P < 0.06) and rapid (67 vs. 46%, NE and EI, respectively; P = 0.04) sperm were greater from bulls grazing NE than EI bulls in July and August. Average velocity of the smoothed sperm path and progressive velocity in a straight line from the beginning to the end of the sperm track were slower (P < 0.09) in EI bulls than NE bulls and were slower (P = 0.04) in August compared with July. Mean width of head oscillation as the sperm swims was less (P < 0.06) in August than July. Concentrations of cortisol and testosterone were not (P > 0.10) influenced by fescue type. Semen from bulls grazing EI had reduced motility and morphology than bulls grazing NE. Detrimental effects of toxic fescue may not be mediated by cortisol, testosterone, or both. Semen quality of bulls grazing toxic EI tall fescue was decreased with increased maximum ambient temperatures.

8 ANTI-MLLERIAN HORMONE AT WEANING AND BREEDING AS A PREDICTOR OF BEEF HEIFER FERTILITY

This study examined the relationship between serum anti-Müllerian hormone (AMH) concentration at weaning and breeding to determine if either or both measures could predict subsequent fertility of beef heifers. Blood was collected from 71 Angus based heifers at ~7 and 14 months of age and serum stored frozen (-20°C) until analysis for AMH, using bovine AMH ELISA kits (AL115; Ansh Laboratories, Webster, TX, USA). Before breeding, transrectal ultrasonography was used to determine cyclic status of heifers. Heifers received 25mg of prostaglandin F2α (PGF) and those detected in oestrus were AI ~12h after detected oestrus. Heifers not detected in oestrus after 7 days received a second PGF injection and oestrus detection and AI continued for 4 days. Ten days later, the heifers were exposed to fertile bulls for a 45-days breeding season. At 50 to 60 days after insemination, transrectal ultrasonography was used to identify pregnant heifers and to confirm conception date based on fetal size. At 60 days after bull removal, ultrasonography was performed to determine pregnancy in heifers conceiving during the breeding season and confirm continuing pregnancy in heifers previously identified as pregnant. Based on fetal size at ultrasonography, the oestrous cycle after initiation of breeding when conception occurred was estimated. For comparison, AI pregnancies were considered cycle 1, and pregnancies initiated during subsequent 21-day intervals of the breeding season were classified as cycles 2, 3, and 4, respectively. For analysis, frequency distribution was used to assign serum AMH concentration measured at weaning, breeding, and the change from weaning to breeding, into quartiles. Comparisons were then made via Chi-squared analysis for heifers in each quartile that were cyclic at synchronization, detected in oestrus, conceived after AI, pregnant at the end of the breeding season, and the estimated cycle that conception occurred. Comparison of heifers based on serum AMH quartiles at weaning failed (P ≥ 0.35) to detect any effect of AMH on subsequent heifer cyclicity at breeding, oestrous response after synchronization, AI conceptions, overall pregnancy rate, or estimated oestrous cycle of the breeding season when conception occurred. Based on AMH concentration at breeding, heifers in the lowest quartile (Q1) had a lower (10%; P=0.02) AI conception rate than heifers in other quartiles (43 to 73%), and conceived at a later oestrous cycle (P=0.03) in the breeding season. Comparison of heifers based on the change in AMH concentrations from weaning to breeding revealed that none of the heifers in the lowest quartile (Q1) conceived after AI, compared with 80% in the highest quartile (Q4; P<0.001). Heifers in the lowest quartile also conceived at a later oestrous cycle in the breeding season than heifers in the other quartiles (P=0.01). Results indicate that either AMH concentration at breeding or the change in AMH from weaning to breeding can identify beef heifers more likely to conceive to AI and to conceive early in the breeding season.