T. Rowe

Low Variability of Intraindividual Longitudinal Leukocyte Gene Expression Profiling Cardiac Allograft Rejection Scores

with ET-Kyoto solution before pancreas preservation significantly improved islet yield from both deceased donor and autologous live pancreata (4, 9). Both collagenase infusion and DI require a pancreatic duct, therefore, an opened main pancreatic duct as created by the Puestow procedure is a major problem. To overcome this issue, we reconstructed the pancreatic duct and jejunum conduit using autosuture. This simple procedure made it possible for us to reconstruct a watertight conduit for collagenase infusion and DI. This is the first report to show that the reconstruction of the pancreatic duct resulted in successful islet isolation and, in turn, insulin independence. In addition, duct reconstruction could be applicable to posttraumatic pancreatectomy cases in which a laceration or damage to the duct could be present (10). Bacteria contamination is a concern using this technique. In this case, bacteremia was not observed; however, we recommend careful observation for bacteremia. Even if pancreatitis is in its advanced phase, TP with AIT could effectively reduce abdominal pain and maintain excellent glycemic control as long as patients receive a sufficient quantity of islets (11). This reconstruction method should be useful to promote TP with AIT after Puestow procedure for patients who have advanced pancreatitis.