T.Y Kim

Global physiological understanding and metabolic engineering of microorganisms based on omics studies

Through metabolic engineering, scientists seek to modify the metabolic pathways of living organisms to facilitate optimized, efficient production of target biomolecules. During the past decade, we have seen notable improvements in biotechnology, many of which have been based on metabolically engineered microorganisms. Recent developments in the fields of functional genomics, transcriptomics, proteomics, and metabolomics have changed metabolic engineering strategies from the local pathway level to the whole system level. This article focuses on recent advances in the field of metabolic engineering, which have been powered by the combined approaches of the various “omics” that allow us to understand the microbial metabolism at a global scale and to develop more effectively redesigned metabolic pathways for the enhanced production of target bioproducts.

Phylogenetic analysis based on genome-scale metabolic pathway reaction content

Phylogenetic classifications based on single genes such as rRNA genes do not provide a complete and accurate picture of evolution because they do not account for evolutionary leaps caused by gene transfer, duplication, deletion and functional replacement. Here, we present a whole-genome-scale phylogeny based on metabolic pathway reaction content. From the genome sequences of 42 microorganisms, we deduced the metabolic pathway reactions and used the relatedness of these contents to construct a phylogenetic tree that represents the similarity of metabolic profiles (relatedness) as well as the extent of metabolic pathway similarity (evolutionary distance). This method accounts for horizontal gene transfer and specific gene loss by comparison of whole metabolic subpathways, and allows evaluation of evolutionary relatedness and changes in metabolic pathways. Thus, a tree based on metabolic pathway content represents both the evolutionary time scale (changes in genetic content) and the evolutionary process (changes in metabolism).

A method to find an optimal draw solute for cost-effective FO(forward osmosis) desalination process

Abstract A method to find a draw solute for forward osmosis desalination is developed. The economics of a forward osmosis process is largely influenced by the selection of a draw solute and energy consumed for its separation from the fresh product water. The proposed method evaluates a vast number of possible draw solutes by predicting the production rate of fresh water, investment cost and energy consumption in the separation process. The possible draw solutes are confined to electrolytes and its separation processes to thermal systems. Subsequently, a cost-minimizing draw solute is determined subject to any necessary constraints. The developed method will contribute to designing commercially viable forward-osmosis desalination processes.