Tadashi Miyake

Specific binding proteins of thyroxine and triiodothyronine in liver soluble proteins.

Abstract 1. 1. The binding of l -thyroxine (T 4 ) and 3, 5, 3′-triiodo- l -thyronine (T 3 ) by rat serum and liver soluble proteins was studied using DEAE-cellulose chromatography and gel filtration on Sephadex G-100. 2. 2. The methods employed provided clear evidence for specific T 4 - and T 3 - binding proteins, which differed from each other, in the liver soluble fraction. 3. 3. Gel filtration studies indicated that both cellular T 4 -binding protein and T 3 -binding protein were excluded by Sephadex G-100 gel in contrast with serum T 4 -binding protein, and therefore, their molecular weights were considered greater than 100 000. 4. 4. The sorption effect of Sepahdex for the thyroid hormones permitted comparison of the relative binding affinities of proteins for the hormones. The liver soluble proteins showed considerably lower for T 4 , but nearly the same order of affinity for T 3 as compared with serum proteins. Cellular T 4 -binding protein and T 3 -binding protein had the highest affinities for corresponding hormones in the liver soluble proteins. 5. 5. The results suggested that the cellular T 4 -binding protein and T 3 -binding protein could play a major role in the hepatic uptake and intracellular transport of separate hormones, and that an intracellular mechanism of T 3 transport may differ somewhat from that of T 4 . 6. 6. It was demonstrated that serum T 4 -binding protein of rats was different from albumin, and its molecular weights is probably lower than that of albumin.

ALZHEIMER'S DISEASE: A CASE REPORT WITH CEREBRAL BIOPSY AND ENYMATIC-HISTOCHEMICAL OBSERVATIONS

Alzheimer病における老人斑の成因は,近年老化現象と関連して注目されているが,なお明らかでない.著者らは脳生検による新鮮資料を用いて種々の酵素組織化学的検素を行なつた.症例は6年前より進行性の痴呆を示す57才の男で,高度の精神荒廃と左上・下肢の筋強剛および軽度の健忘性失語症,構成失行,左右障害を認めた.リコール蛋白分画の異常,脳波の全般性徐波,気脳写による高度の脳萎縮像等がみられた.前頭葉より得た生検資料により多数の嗜銀性老人斑を証明し, Alzheimer病の診断を確認した。しかしAlzheimer原線維変化はみられなかつた.老人斑のあるものはPAS陽性であつた.succinic dehydrogenase及びDPN diaphoraseは老人斑に活性の上昇を示さなかつたが, acid phosphataseは老人斑及びその周辺の小型細胞中に強い活性を示した.これは老人斑の沈澱物に対するmicroglia及び組織球の反応性増生を示唆する.この種の試みとしてはFriedeらに次ぐ第2例目の報告であり, Friedeらの成績と比較し考察を加えた.