Tadashi Nagamatsu

実験的腎炎ラットに関する薬理学的研究(第11報) 抗ラット GBM ウサギ血清による腎炎の病理組織と生化学的パラメーターの変動

Anti-rat glomerular basement membrane (GBM) rabbit serum was produced by immunizing rabbits with the supernatant substance of trypsin-digested rat GBM. Nephritis was induced in rats by a single intravenous administration of 0.25 ml of anti-serum and changes in pathohistological and biochemical parameters during the process of the disease were investigated in comparison with those of Masugi nephritis and the modified type of Masugi nephritis previously reported. In light microscopic studies, histological changes seen in the kidneys closely resembled those of typical human glomerulonephritis. Changes such as hypercellularity, adhesion between capillary wall and Bowmans capsule, crescent formation and hyalinization in glomeruli and interstitial infiltration were the most pronounced on the 30th day after the anti-serum injection. In immunofluorescent studies, a linear fixation of rabbit IgG was observed along the GBM from the 1st day and the staining of a certain intensity was preserved throughout the experimental periods. A linear staining with anti-rat IgG serum was recognized from the 10th day. The fixation of fibrinogen was also seen in not only the glomerular capillary walls, but also in Bowmans space after the 10th day. Proteinuria significantly increased from the 1st day, reached a peak of 12 times the control level, and thereafter gradually decreased. The patterns of progress of urinary alkaline phosphatase and N-acetyl-beta-glucosaminidase activities were much the same as those seen in cases of proteinuria and the levels at their peak times were about 10 and 3 times control levels, respectively. Plasma urea nitrogen level transiently increased on the 5th day and then reverted to the control level by the 30th day. Plasma cholesterol levels were significantly high from the 5th to the 20th days. It is concluded that glomerular damages in this model are more severe, so-called, nephritic type and continue for longer periods than in cases of Masugi nephritis, however, do not differ in degree and duration from findings in the modified type of Masugi nephritis.

Anti-nephritic effects of prostaglandin E1 on serum sickness nephritis in rat

結 果 (1)尿 中パ ラメータに対す る効果(図1) (I)蛋 白尿:PGE1で の治療開始7日 目において,皿 群 のみが治療開始前 と比較 して45%の 有意 な 減少を示 し た.一 方,II・III群 では1群 に対 しおのおの20~30%の 治癒率を示 した. II ALP活 性:1群 では経 日的 に上昇 したのに比 し, 1皿群では減少を示 し,7日 目には1群 に対 し約40%の 有 意な治癒率を示 した. (2)血 中 パラメータに対す る効果(図2) I群 はBUNお よびcho.値 とも10日 間の実験 期間を 通 しておのおの約45mg/dlお よび250mg/dlの 高値を 持続 した.こ れ に対 してII・III群では両パ ラメータとも 治療 開始前 の値 と比較 して,5,10日 目に有意 に減少 し た.ま た10日 目のBUNに おいてII・III群では1群 に対 し約70~80%の 有意 な治癒 率を示 した. (3)腎 糸球体組織学 的パ ラメータに対す る効果 (I)光学顕微鏡所見:図 は省略 したが,PGE1処 置後10 日目において1群 では糸球体の毛細血 管内白血球 に よる 富核,メ サ ソジウム基 質の増加,係 蹄壁の肥厚お よび腫 大 が顕著 であった.II・III群 では1群 に比 していずれの 所見 もかな りの改善 を示 した. II螢光抗体所見(表1):RSA,IgG,IgMは 主 にメ サ ンジウムに局在 し,大 きな塊 として観察 され,糸 球体