Tadato Tani

Quantitative determination of amygdalin epimers by cyclodextrin-modified micellar electrokinetic chromatography.

A new capillary electrophoresis method was developed for the quantitative determination of the amygdalin epimers, amygdalin and neoamygdalin, which are biologically significant constituents in the crude drugs, namely Persicae Semen and Armeniacae Semen. The effects of surfactants, additives and other analytical parameters were studied. As a result, the resolution of two epimers was performed by cyclodextrin-modified micellar electrokinetic chromatography with a buffer containing alpha-cyclodextrin and sodium deoxycholate. By the application of this method, a simple, fast and simultaneous quantitative determinations of amygdalin epimers in the crude drugs (Persicae Semen and Armeniacae Semen) and the Chinese herbal prescriptions (Keishi-bukuryo-gan and Mao-to) were achieved.

Histochemistry : IX. Distribution of saikosaponins in bupleurum falcatum root

Abstract The root of Bupleurum falcatum cultivated in Japan was examined by histological and chemical means in order to clarify the distribution of saikosaponins in the various kinds of tissues and parts of the root. By means of qualitative thin-layer chromatographic—densitometric and quantitative high-performance liquid chromatographic analyses, saikosaponins, bioactive principles in the root, were found to be abundant in the outer phloem layer, especially the pericycle and its neighbouring parenchyma cells in the root. It was also found that the highest level of saikosaponins was detected in thinner root hairs and that the level decreased towards the thicker root head.