Tae-Kwang Oh

Nocardioides dubius sp. nov., isolated from an alkaline soil

A Gram-positive, rod- or coccus-shaped bacterial strain, KSL-104(T), was isolated from an alkaline soil from Korea and its taxonomic position was investigated by a polyphasic approach. Strain KSL-104(T) grew optimally at pH 7.0-8.0 and 30 degrees C. It was characterized chemotaxonomically as having a cell-wall peptidoglycan type based on ll-2,6-diaminopimelic acid with MK-8(H(4)) as the predominant menaquinone. The major fatty acid was iso-C(16:0) and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The DNA G+C content was 70.6 mol%. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain KSL-104(T) joined the cluster comprising Nocardioides jensenii and Marmoricola aurantiacus. The cellular fatty acid profile of strain KSL-104(T) was different from that of M. aurantiacus. Strain KSL-104(T) and the type strain of N. jensenii exhibited a 16S rRNA gene sequence similarity value of 97.1% and a mean DNA-DNA relatedness value of 13%. Levels of 16S rRNA gene sequence similarity between strain KSL-104(T) and the type strains of other Nocardioides species were in the range 94.0-96.5%. On the basis of phenotypic, phylogenetic and genetic data, strain KSL-104(T) (=KCTC 9992(T)=JCM 13008(T)) represents a novel species of the genus Nocardioides, for which the name Nocardioides dubius sp. nov. is proposed.

Halobacillus locisalis sp. nov., a halophilic bacterium isolated from a marine solar saltern of the Yellow Sea in Korea.

A Gram-positive, motile, endospore-forming and rod-shaped halophilic bacterial strain MSS-155 (KCTC 3788 and KCCM 41687) was isolated from a marine solar saltern of the Yellow Sea in Korea and was subjected to a polyphasic taxonomic study. This organism grew at temperature of 10.0–42.0°C with an optimum of 35°C. Strain MSS-155 grew optimally in the presence of 10% NaCl and did not grow in the absence of NaCl. The cell wall peptidoglycan type of strain MSS-155 was A4β based on l-Orn-d-Asp. Strain MSS-155 was also characterized chemotaxonomically by having menaquinone-7 (MK-7) as the predominant isoprenoid quinone and anteiso-C15:0 as the major fatty acid. The DNA G+C content was 44.0xa0mol%. Phylogenetic analysis based on 16S rDNA sequences showed that strain MSS-155 falls within the radiation of the cluster comprising Halobacillus species. Levels of 16S rDNA sequence similarity between strain MSS-155 and the type strains of four Halobacillus species were in the range 97.6–98.8%. Strain MSS-155 exhibited levels of DNA-DNA relatedness of 6.2–11.2% to the type strains of Halobacillus species described previously. On the basis of phenotypic properties, phylogeny, and genomic data, strain MSS-155 should be placed in the genus Halobacillus as a member of a novel species, for which we propose the name Halobacillus locisalis sp. nov.