Taek-Won Ahn

Neuroprotective Effects of Hyangsayangwi-tang in MPTP-induced Mouse Model of Parkinson's Disease

Objectives To evaluate the neuroprotective effects of Hyangsayangwi-tang (HY), a Korean traditional medicinal prescription in a Parkinsons disease mouse model. Methods Four groups(each of 10 mouse per group) were used in this study. The neuroprotective effect of HY was examined in a Parkinsons disease mouse model. C57BL/6 mouse treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30mg/kg/day), intraperitoneal (i.p.) for 5 days. Slow behavioral responses and memory disorder is the major clinical symptoms of PD. In order to investigate the effect of HY on recovery of behavioral deficits and memory, we examined the motor function and memory by using Morris water maze and Forced swimming test. Ischemic mouse brain stained with TTC(2,3,5 triphenyl tetrazolium chloride) in the MPTP-induced Parkinsons disease to find out ischemia and tissue damage in mouse. The convenient, simple, and accurate high-performance liquid chromatography (HPLC) method was established for simultaneous determination of neurotransmitters in MPTP-HY group. To measure the amount of dopamine in mice brain, striatum-substantia nigra, was examined by Bradford assay. Immunohistochemistry was examined in the MPTP-induced Parkinsons disease (PD) mouse to evaluate the neuroprotective effects of Hyangsayangwi-tang on hippocampal lesion, ST and SNpc. Results and Conclusions Hyangsayangwi-tang (HY) prevents MPTP-induced loss of serotonin, hippocampus and TH-ir cell.

A Case Study about Soyangin Cerebellar Atrophy Patient suffering from Dizziness improved by Hyeungbangjihwang-tang

Objectives This case is about a Soyangin cerebellar atrophy patient. In this study, we report the effect of Sasang Constitutional treatment to this patient. Methods This patient was treated by Soyangin`s constitutional medications and acupuncture according to the result of Sasang Constitutional diagnosis. We used Visual Analogue Scale(VAS) and Equilibrium Function Test. Results and Conclusions This patient`s chief complaints were dizziness. By using Hyeungbangjihwang-tang, she showed positive response about her symptom. This study shows that Hyeungbangjihwang-tang has effect to cerebellar atrophy in Soyangin.

Neuroprotective Effects of Modified Yuldahanso-tang (MYH) in a Parkinson's Disease Mouse Model

Neuroprotective Effects of Modified Yuldahanso-tang (MYH) in a Parkinsons Disease Mouse Model Ga-Yeon Go, Yoon-Ha Kim, Taek-Won Ahn Dept. of Sasang Constitutional Medicine, College of Oriental Medicine, Daejeon Univercity Objectives To evaluate the neuroprotective effects of modified Yuldahanso-tang (MYH) in a Parkinsons disease mouse model. Methods 1) Four groups (each of 8 rats per group) were used in this study. 2) The neuroprotective effect of MYH was examined in a Parkinsons disease mouse model. C57BL/6 mice treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg/day), intraperitoneal (i.p.) for 5 days. 3) The brains of 2 mice per group were removed and frozen at -20°C, and the striatum-substantia nigra part was seperated. The protein volume was measured by Bradford method following Bio-Rad protein analyzing kit. Using mouse/Rat Dopamine ELISA Assay Kit. 4) The brains of 2 mice per group were separated and removed. TH-immunohistochemical was examined in the MPTP-induced Parkinsons disease mice to evaluate the neuroprotective effects of MYH on ST and SNpc. 5) Two mice out of each group were anesthetized and skulls were opened from occipital to frontal direction to take out the brains. The brains added TTC solution for 20 minutes for staining. 6) The water tank used for morris water maze test was filled with 28°C water, and a round platform of 10cm in diameter was installed for mice to step on. The study was carried out once a day within 30 seconds, keep exercising to step on the platform in the pool. 7) The brains of two mice out of each group were fixed in 10% formaldehyde solution and paraphillin substance was infiltrated. They were fragmented by microtome, and observed under an optical microscope after Hematoxylin & Eosin staining. 8) A round acrylic cylinder with its upper side open was filled with clean water and depressive mouse models were forced to swim for 15 minutes. After 24 hours the animals were put in the same equipment for 5 minutes and were forced to swim. 9) The convenient, simple, and accurate high-performance liquid chromatography (HPLC) method was established for simultaneous determination of Neurotransmitters in MPTP-MYH group. Results 1) MYH possess Dopamine cell protective effect on MPTP-induced injury in striatum and substantia nigra pars compacta. 2) MYH inhibits the loss of tyrosine hydroxylase-immunoreacitive (TH-IR) cells in the striatum and substantia nigra pars compacta on MPTP-induced injury in C57BL/6 mice.

2 Case Reports on Oro-facial Dyskinesia Patients Diagnosed and Treated in Sasang Constitutional Medicine

Abstract 2 Case Reports on Oro-facial Dyskinesia Patients Diagnosed and Treated in Sasang Constitutional Medicine Jung-Hwan Park, Yoon-Ha Kim, Ga-Yeon Go, Taek-Won AhnDept. of Sasang Constitutional Medicine, College of Oriental Medicine, Daejeon UnivercityObjectivesThis study was designed to report diagnosis and treatment by Sasang Constitutional Medicine to the patients with Oro-facial Dyskinesia.Methods These two patients were diagnosed as Soyangin and Taeeumin according to the result of Sasang constitutional diagnosis, and treated by Sasang constitutional medications and acupuncture. The progress was evaluatedwith the Global Assessment Scale (GAS).ResultOro-facial Dyskinesia was almost disappeared in each patients. the one patient was cured with Chongshimyeonja-tang and Yuldahanso-tang, and the other was cured with Hyeungbangjihwang-tang. ConclusionWe can treat Oro-facial Dyskinesia whose cause is mostly idiopathic with the diagnosis and treatment of Sasang Constitutional Medicine.Key Words: Oro-facial Dyskinesia, Chongshimyeonja-tang, Yuldahanso-tang, Hyeungbangjihwang-tang.

A Case Report on Parkinson`s Disease Soyangin Patient with Abdominal Pain and Constipation

A Case Report on Parkinson’s Disease Soyangin Patient with Abdominal Pain and Constipation Jinyoung Kwak*, Yoon-Ha Kim*, Jung-Hwan Park*, Seo-jin Hong†, Taek-Won Ahn* *Dept. of Sasang Constitutional Medicine, College of Oriental Medicine, Daejeon University †Cheonan Oriental Hospital of Daejeon University Objectives The purpose of this case was to report significant improvement of abdominal pain, constipation and a slight improvement of tremor after treatment by Sasang Constitutional Medicine to the patient with Parkinson’s disease. Methods The patient was diagnosed as Soyangin according to the result of Sasang constitutional diagnosis, and treated by Sasang constitutional medications and acupuncture. The progress was evaluated with the Global Assessment Scale (GAS). Results The abdominal pain was reduced from Numeric Rating Scale (NRS)9 to NRS1 and headache was reduced from NRS5 to NRS3. The tremor was reduced to GAS 10. Unified Parkinson Disease Rating Scale (UPDRS) was reduced from 40 to 27. The difference of heat distribution between left and right side was reduced and the abdomen was more warmer on Digital Infrared Thermal Imaging (DITI). Conclusions Sasang constitutional medicine can be a solution for the non motor symptoms caused by Parkinson’s disease.

A study on the effect on obesity and lipid metabolism in liver hypofunction animal-experimental model induced by Acetaminophen(AAP) injection

Objectives: The objective of this research is to develop new animal-experimental model for Sasang Constitutional Medicine, especially for partial Taeyangin(one of four constitution which has good pulmonary function and poor hepatic function) by AAP intraperitoneal injection, and to estimate from the viewpoint of obesity and lipid metabolism. Methods: The C57bl/6J mice was divided into 4 groups ; Normal group, AAP group, High-Fat-Diet(HFD) group, and HFD+AAP group. 200mg AAP was injected intraperitoneally to the AAP group twice a week for six weeks, and HFD group was fed with 60%-High-fat Diet for six weeks. HFD+AAP group got both AAP injection and 60%-High-fat Diet at the same time for the same period. In this period, We measured the weight and Food Efficiency Ratio(FER, %) once a week. After six weeks, We conducted the blood chemical test from the groups, and extracted the fat tissue to measure weight. Results & conclusion: In the liver function test, two AAP groups had higher AST and ALP, and normal LDH. The blood level of creatinine from all groups were normal. The rate in weight was lesser by 7.8% in HFD+AAP group, and had lesser FER than HFD group. Also They had lesser Total cholesterol and LDL cholesterol, and had more HDL cholesterol than HFD group. HFD+AAP group hadmore glucose in serum and lesser Insulin-like Growth Factor 1(IGF-1) than HFD group.