Takahiro Motoyoshi

Development of a real-time local environment stimulation system with visual feedback control

Single cell analysis has attracted much attention for revealing detailed and localized biological information. A local environmental control technique is desired when analyzing the detailed and localized properties of single cells. In this paper, we propose a local environmental stimulation system with micro dual-pipettes to stimulate the local reagent concentration dynamically, freely and automatically. When the local environment is controlled with chemical stimuli, the chemical solution is diffused and affects other objects. The local environment stimulation system with micro dual-pipettes can solve this problem by using spout pipette and suction pipette. As a cell analysis system, system quantification is necessary, so we try to realize it using a fluorescent substance. First step, we try to measure and control the light intensity. As an application of the system, the system can construct two-dimensional cell-organization by peeling off certain area of cell-sheet. Therefore, we try to control the area of peeling off cells using the system.

Real-time local environmental stimulation system with visual feedback control

Single cell analysis has attracted much attention for revealing detailed and localized biological information. A local environmental control technique is desired when analyzing the detailed and localized properties of single cells. In this paper, we propose a local environmental stimulation system which causes chemical stimulation in local area with micro dual-pipettes dynamically, freely, and automatically. As a cell analysis system, system quantification is necessary, so we try to realize it by using a fluorescent substance. Moreover, combining the spouted pipette and sucked pipette, we propose more localized stimulating system. As a first step, we try to measure the light intensity of the fluorescent substance using this system.

Control of flagellar motor using a real-time local environment chemical stimulation system

Single cell analysis has attracted much attention to reveal the localized biological information in detail. Local environmental control technique is developed to analyze the localized detail properties of single cells. In this paper, we propose the local environmental chemical stimulation system with micro dual pipettes to stimuli the local reagent concentration dynamically and automatically. Local environmental chemical stimulation by dual pipettes is applied to the rotational speed control of bacterial flagellar motor, which is a rotary molecular machine. Here, we show quick response and rotational speed control of Na-driven flagellar motor in both accelerating and relaxing directions was demonstrated by automatic switching the local spout between Na-containing and Na-free solutions with dual pipettes. It was shown that the rotational speed could be maintained by automatic controlling the spouting velocity of Na-containing and Na-free solution with multiplying the applied DC voltage. Furthermore, it was confirmed that by applying the P-control, rotational speed of flagellar motor could be controlled more stably.

Development of chemical stimulation system for local environment control by using combination of spout and suction from dual-pipettes.

Single cell analysis has attracted much attention for revealing detailed and localized biological information. A local environmental control technique is desired when analyzing the detailed and localized properties of single cells. In this paper, we propose a local environmental stimulation system with micro dual-pipettes to stimulate the local reagent concentration dynamically, freely and automatically. When the local environment is controlled with chemical stimuli, the chemical solution is diffused and affects other objects. The local environment stimulation system with micro dual-pipettes can solve this problem. By using spout pipette and suction pipette, it is possible to use local stimuli without affecting other objects. For evaluating the locality of the system, we use a fluorescent substance and measure the diffusion of the spouted solution.