Takako Honda
National Institute for Materials Science
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Takako Honda.
PLOS ONE | 2015
Yoshihide Hashimoto; Seiichi Funamoto; Shuji Sasaki; Jun Negishi; Takako Honda; Shinya Hattori; Kwangwoo Nam; Tsuyoshi Kimura; Manabu Mochizuki; Hisatoshi Kobayashi; Akio Kishida
The purpose of this study is to demonstrate the feasibility of DALK using a decellularized corneal matrix obtained by HHP methodology. Porcine corneas were hydrostatically pressurized at 980 MPa at 10°C for 10 minutes to destroy the cells, followed by washing with EGM-2 medium to remove the cell debris. The HHP-treated corneas were stained with H-E to assess the efficacy of decellularization. The decellularized corneal matrix of 300 μm thickness and 6.0 mm diameter was transplanted onto a 6.0 mm diameter keratectomy wound. The time course of regeneration on the decellularized corneal matrix was evaluated by haze grading score, fluorescein staining, and immunohistochemistry. H-E staining revealed that no cell nuclei were observed in the decellularized corneal matrix. The decellularized corneal matrices were opaque immediately after transplantation, but became completely transparent after 4 months. Fluorescein staining revealed that initial migration of epithelial cells over the grafts was slow, taking 3 months to completely cover the implant. Histological sections revealed that the implanted decellularized corneal matrix was completely integrated with the receptive rabbit cornea, and keratocytes infiltrated into the decellularized corneal matrix 6 months after transplantation. No inflammatory cells such as macrophages, or neovascularization, were observed during the implantation period. The decellularized corneal matrix improved corneal transparency, and remodelled the graft after being transplanted, demonstrating that the matrix obtained by HHP was a useful graft for corneal tissue regeneration.
Scientific Reports | 2016
Yoshihide Hashimoto; Shinya Hattori; Shuji Sasaki; Takako Honda; Tsuyoshi Kimura; Seiichi Funamoto; Hisatoshi Kobayashi; Akio Kishida
The decellularized cornea has received considerable attention for use as an artificial cornea. The decellularized cornea is free from cellular components and other immunogens, but maintains the integrity of the extracellular matrix. However, the ultrastructure of the decellularized cornea has yet to be demonstrated in detail. We investigated the influence of high hydrostatic pressure (HHP) on the decellularization of the corneal ultrastructure and its involvement in transparency, and assessed the in vivo behaviour of the decellularized cornea using two animal transplantation models, in relation to remodelling of collagen fibrils. Decellularized corneas were prepared by the HHP method. The decellularized corneas were executed by haematoxylin and eosin and Masson’s trichrome staining to demonstrate the complete removal of corneal cells. Transmission electron microscopy revealed that the ultrastructure of the decellularized cornea prepared by the HHP method was better maintained than that of the decellularized cornea prepared by the detergent method. The decellularized cornea after interlamellar keratoplasty and microkeratome-assisted anterior lamellar keratoplasty using a rabbit model was stable and remained transparent without ultrastructural alterations. We conclude that the superior properties of the decellularized cornea prepared by the HHP method were attributed to the preservation of the corneal ultrastructure.
Advanced Materials Research | 2011
Chih-Feng Huang; Chiaki Yoshikawa; Kun Zhang; Shinya Hattori; Takako Honda; Ewelina Zawadzak; Hisatoshi Kobayashi
In this research, we aimed to develop a new type of core-shell electrospun fiber, possessing “short” length (core) and “concentrated” polymer brush (shell). We prepared electrospun fibers with initiating moiety for surface-initiated atom transfer radical polymerization (SI-ATRP), one of living radical polymerizations. Then we grafted poly(sodium styrene sulfonate) (PSSNa) on the fibers by SI-ATRP. After the polymerization, we mechanically cut the electrospun fibers with a homogenizer, yielding regulated shortened fibers. Our ultimate goal is to make a novel shortened nanofibril biomaterial with concentrated brush as cell growth scaffolds. Therefore we expect that this unprecedented short nanofiber can be broadly applied as a biomaterial owing to the unique structures and properties of the concentrated brush. The details will be discussed.
Microscopy Research and Technique | 2013
Dohiko Terada; Shinya Hattori; Takako Honda; Masanori Iitake; Hisatoshi Kobayashi
The focused ion beam (FIB) technology has drawn considerable attention in diverse research fields. FIB can be used to mill samples at the nanometer scale by using an ion beam derived from electrically charged liquid gallium (Ga). This powerful technology with accuracy at the nanometer scale is now being applied to life science research. In this study, we show the potential of FIB as a new tool to investigate the internal structures of cells. We sputtered Ga+ onto the surface or the cross section of animal cells to emboss the internal structures of the cell. Ga+ sputtering can erode the cell surface or the cross section and thus emboss the cytoskeletons quasi‐3 dimensionally. We also identified the embossed structures by comparing them with fluorescent images obtained via confocal laser microscopy because the secondary ion micrographs did not directly provide qualitative information directly. Furthermore, we considered artifacts during the FIB cross sectioning of cells and propose a way to prevent undesirable artifacts. We demonstrate the usefulness of FIB to observe the internal structures of cells. Microsc. Res. Tech. 76:290–295, 2013.
Biomaterials | 2010
Yoshihide Hashimoto; Seiichi Funamoto; Shuji Sasaki; Takako Honda; Shinya Hattori; Kwangwoo Nam; Tsuyoshi Kimura; Manabu Mochizuki; Toshiya Fujisato; Hisatoshi Kobayashi; Akio Kishida
Molecular Vision | 2009
Shuji Sasaki; Seiichi Funamoto; Yoshihide Hashimoto; Tsuyoshi Kimura; Takako Honda; Shinya Hattori; Hisatoshi Kobayashi; Akio Kishida; Manabu Mochizuki
Chemistry Letters | 2010
Chiaki Yoshikawa; Yoshihide Hashimoto; Shinya Hattori; Takako Honda; Kun Zhang; Dohiko Terada; Akio Kishida; Yoshinobu Tsujii; Hisatoshi Kobayashi
Materials Letters | 2012
Chiaki Yoshikawa; Shinya Hattori; Takako Honda; Chih-Feng Huang; Hisatoshi Kobayashi
Bioinspired, biomimetic and nanobiomaterials | 2012
Shinya Hattori; Dohiko Terada; Andreana Bibin Bintang; Takako Honda; Chiaki Yoshikawa; Hidetoshi Teramoto; Tsunenori Kameda; Tamada Yasushi; Hisatoshi Kobayashi
In Commemoration of the 1st Asian Biomaterials Congress | 2008
Hisatoshi Kobayashi; Yoshiro Yokoyama; Chiaki Yoshikawa; Satoshi Igarashi; Shinya Hattori; Takako Honda; Hiroyuki Koyama; Tsuyoshi Takato