Takashi Maeda | Researchain

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Featured researches published by Takashi Maeda.

Growth Factors Journal | 2009

Human eosinophil cationic protein enhances stress fiber formation in Balb/c 3T3 fibroblasts and differentiation of rat neonatal cardiomyocytes.

Takayuki Fukuda; Miki Iwata; Midori Kitazoe; Takashi Maeda; David S. Salomon; Satoshi Hirohata; Katsuyuki Tanizawa; Shun'ichi Kuroda; Masaharu Seno

We found that eosinophil cationic protein (ECP) stimulated the growth of mouse Balb/c 3T3 fibroblasts. ECP-treated 3T3 cells were more flattened and exhibited enhanced stress fiber formation. The enhancement of cytoskeleton after addition of recombinant ECP appeared stable and was able to inhibit disassembly of actin filaments that was induced by fibroblast growth factor-2. The ROCK inhibitor, Y-27632, abrogated this enhancement on stress fiber formation that was induced by ECP indicating the involvement of Rho/ROCK signaling pathway. The effect of ECP was assessed on the differentiation of primary cardiomyocytes derived from rat neonatal heart since the development of actin filaments is significantly related with organization of stress fibers. As the result, both beating rate and the expression of cardiac muscle specific markers such as atrial natriuretic factor were enhanced in the presence of ECP. Thus ECP may also function as a cardiomyocyte differentiation factor.

Biochemistry | 2001

Preparation of potent cytotoxic ribonucleases by cationization : enhanced cellular uptake and decreased interaction with ribonuclease inhibitor by chemical modification of carboxyl groups

Junichiro Futami; Takashi Maeda; Midori Kitazoe; Emiko Nukui; Hiroko Tada; Masaharu Seno; Megumi Kosaka; Hidenori Yamada

Journal of Bioscience and Bioengineering | 2005

Intracellular Delivery of Proteins into Mammalian Living Cells by Polyethylenimine-Cationization

Junichiro Futami; Midori Kitazoe; Takashi Maeda; Emiko Nukui; Masakiyo Sakaguchi; Jun Kosaka; Masahiro Miyazaki; Megumi Kosaka; Hiroko Tada; Masaharu Seno; Junzo Sasaki; Nam Hu Huh; Masayoshi Namba; Hidenori Yamada

FEBS Journal | 2002

Growth inhibition of mammalian cells by eosinophil cationic protein

Takashi Maeda; Midori Kitazoe; Hiroko Tada; Rafael de Llorens; David S. Salomon; Masakazu Ueda; Hidenori Yamada; Masaharu Seno

Journal of Molecular Biology | 2000

Three-dimensional crystal structure of human eosinophil cationic protein (RNase 3) at 1.75 Å resolution

Goretti Mallorquí-Fernández; Joan Pous; Rosa Peracaula; Joan Aymamí; Takashi Maeda; Hiroko Tada; Hidenori Yamada; Masaharu Seno; Rafael de Llorens; F. Xavier Gomis-Rüth; Miquel Coll

Journal of Biochemistry | 2002

Optimum Modification for the Highest Cytotoxicity of Cationized Ribonuclease

Junichiro Futami; Emiko Nukui; Takashi Maeda; Megumi Kosaka; Hiroko Tada; Masaharu Seno; Hidenori Yamada

Journal of Biochemistry | 2005

Protein Transduction Assisted by Polyethylenimine-Cationized Carrier Proteins

Midori Kitazoe; Hitoshi Murata; Junichiro Futami; Takashi Maeda; Masakiyo Sakaguchi; Masahiro Miyazaki; Megumi Kosaka; Hiroko Tada; Masaharu Seno; Nam Ho Huh; Masayoshi Namba; Mitsuo Nishikawa; Yoshitake Maeda; Hidenori Yamada

Journal of Biochemistry | 2002

RNase 3 (ECP) Is an Extraordinarily Stable Protein among Human Pancreatic-Type RNases.

Takashi Maeda; Kazutoshi Mahara; Midori Kitazoe; Junichiro Futami; Aiko Takidani; Megumi Kosaka; Hiroko Tada; Masaharu Seno; Hidenori Yamada

Biochemistry | 2006

Denatured and reversibly cationized p53 readily enters cells and simultaneously folds to the functional protein in the cells.

Hitoshi Murata; Masakiyo Sakaguchi; Junichiro Futami; Midori Kitazoe; Takashi Maeda; Hideki Doura; Megumi Kosaka; Hiroko Tada; Masaharu Seno; Nam Ho Huh; Hidenori Yamada

Anticancer Research | 2002