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Journal of Biological Chemistry | 1999

Further Characterization of the Type 3 Ryanodine Receptor (RyR3) Purified from Rabbit Diaphragm

Takashi Murayama; Toshiharu Oba; Eisaku Katayama; Hideto Oyamada; Katsuji Oguchi; Masakazu Kobayashi; Kazuyuki Otsuka; Yasuo Ogawa

We characterized type 3 ryanodine receptor (RyR3) purified from rabbit diaphragm by immunoaffinity chromatography using a specific antibody. The purified receptor was free from 12-kDa FK506-binding protein, although it retained the ability to bind 12-kDa FK506-binding protein. Negatively stained images of RyR3 show a characteristic rectangular structure that was indistinguishable from RyR1. The location of the D2 segment, which exists uniquely in the RyR1 isoform, was determined as the region around domain 9 close to the corner of the square-shaped assembly, with use of D2-directed antibody as a probe. The RyR3 homotetramer had a single class of high affinity [3H]ryanodine-binding sites with a stoichiometry of 1 mol/mol. In planar lipid bilayers, RyR3 displayed cation channel activity that was modulated by several ligands including Ca2+, Mg2+, caffeine, and ATP, which is consistent with [3H]ryanodine binding activity. RyR3 showed a slightly larger unit conductance and a longer mean open time than RyR1. Whereas RyR1 showed two classes of channel activity with distinct open probabilities (P o), RyR3 displayed a homogeneous and steeply Ca2+-dependent activity withP o ∼1. RyR3 was more steeply affected in the channel activity by sulfhydryl-oxidizing and -reducing reagents than RyR1, suggesting that the channel activity of RyR3 may be transformed more precipitously by the redox state. This is also a likely explanation for the difference in the Ca2+ dependence of RyR3 between [3H]ryanodine binding and channel activity.


Archive | 1998

Characteristics of Ryanodine Receptor Type 3 Isoform (RyR3) and Its Homologues

Yasuo Ogawa; Takashi Murayama


生物物理 | 2014

3P139 コネクチン遺伝子の上流から転写される横紋筋タンパク質の解析(10. 筋肉,ポスター,第52回日本生物物理学会年会(2014年度))

Akira Hanashima; Naruki Sato; Sumiko Kimura; Takashi Murayama


生物物理 | 2014

2P302 1 型リアノジン受容体N 末領域における悪性高熱症に関わる機能的変異(27. バイオイメージング,ポスター,第52回日本生物物理学会年会(2014年度))

Toshiko Yamazawa; Takashi Murayama; Hideto Oyamada; Junji Suzuki; Nagomi Kurebayashi; Kazunori Kanemaru; Maki Yamaguchi; Shigeru Takemori; Masamitsu Iino


生物物理 | 2013

3P156 ダイナクチンp150の分子構造(11.分子モーター,ポスター,日本生物物理学会年会第51回(2013年度))

Kei Saito; Takashi Murayama; Tomonori Hata; Yoko Y. Toyoshima


生物物理 | 2013

3P155 Bicaudal-D2による微小管系輸送の制御機構(11.分子モーター,ポスター,日本生物物理学会年会第51回(2013年度))

Takuya Kobayashi; Akira Hanashima; Yoko Y. Toyoshima; Takashi Murayama


生物物理 | 2013

2P201 悪性高熱症関連変異をもたらしている1型リアノジン受容体の機能解析(12.細胞生物的課題,ポスター,日本生物物理学会年会第51回(2013年度))

Toshiko Yamazawa; Takashi Murayama; Hideto Oyamada; Junji Suzuki; Kazunori Kanemaru; Nagomi Kurebayashi; Masamitsu Iino; Shigeru Takemori


Archive | 2013

Over-expression of ryanodine receptor type 1 enhances mitochondrial fragmentation

Jin O-Uchi; Bong Sook Jhun; Stephen Hurst; Sara Bisetto; Polina Gross; Ming Chen; Sarah Kettlewell; Jongsun Park; Hideto Oyamada; Godfrey L. Smith; Takashi Murayama; Shey-Shing Sheu


Archive | 2012

Labeled fusion protein

Eisaku Katayama; Takashi Murayama; Taku Kashiyama; Takuya Kobayashi


生物物理 | 2011

2M1424 哺乳類細胞質ダイニンが示す拡散と一方向性の運動の転換について(分子モーター3,第49回日本生物物理学会年会)

Takayuki Torisawa; Muneyoshi Ichikawa; Tomonori Hata; Takashi Murayama; Yoko Y. Toyoshima

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