Takehisa Matsuda

Ultra long-lifetime and high-sensitive fluorescent measurement using difference compensation method for single cell analysis

In this paper, we proposed long-lifetime and high-sensitive measurement using fluorescent intensity by compensation of photo-bleaching with the difference compensation for cell measurement. Photo-bleaching of fluorescence is one of the most crucial factors to cause measurement error in physiological measurement. In our approach, difference of fluorescent intensity between imaging time is compensated using single exponential function to make the difference amount uniform in each imaging time. We evaluated the dependencies of measurable time and the sensitivity by fluorescent lifetime and the decay coefficient of fluorescent lifetime using numerical simulation. From these results, the proposed method has much higher sensitivity and longer measurable time against conventional compensation method of photo-bleaching. By using the proposed method, we demonstrate the photosynthesis activity of single Synechocystis sp. PCC 6803 in the micro chamber with gas barrier layer.

Fabrication of 3D capillary vessel simulator using femtosecond laser and mask hybrid exposure

Circulation-type blood vessel model can contribute to evaluation of drag delivery systems and the alternative systems for animal test. The shape of conventional models fabricated by photolithography is limited to two dimensions (2D). Therefore, we needed to fabricate three-dimensional (3D) blood vessel models for simulating real blood vessel shapes. For this purpose, we propose a novel 3D exposure method as femtosecond laser and mask hybrid exposure: FMEx. FMEx is integrated two-photon absorption exposure method by using femtosecond laser and photolithography method by using mask aligner. We report the results of fabricated 3D capillary vessel model by using FMEx.