Takeshi Harada

An immunohistochemical study of the extracellular matrix in oral squamous cell carcinoma and its association with invasive and metastatic potential

The expression of extracellular matrices (ECMs) laminin (LN), type IV collagen (IV C), heparansulphate proteoglycan (HS-PG), fibronectin (FN), tenascin (TN), decorin and vitronectin (VN) was examined immunohistochemically in 112 primary tumours and 29 metastatic cervical lymph nodes in oral squamous cell carcinoma (OSCC). In highly invasive primary tumours, the expression of LN, IV C and HS-PG in the basement membrane along the tumour-stroma borderline and the expression of decorin and VN in the tumour stroma at the invasive site were all significantly decreased. The expression of FN and TN in the tumour stroma at the same site was markedly increased. In peritumour stroma in metastatic lymph nodes, LN, IV C, HS-PG, decorin and VN were weakly expressed, while FN and TN were strongly expressed. Thus, the staining pattern of the ECMs in the metastatic lymph nodes was similar to that in highly invasive primary tumours. Furthermore, in primary tumours of metastatic cases, the expression of LN, IV C, HS-PG, decorin and VN obviously decreased, while the expression of FN and TN increased when compared with those of the non-metastatic cases. The investigation of ECMs in OSCC was valuable in predicting tumour behaviour.

Fluorimetric determination of gallium with lumogallion by flow injection analysis based on solvent extraction

Abstract Gallium in the concentration range 4 X 10 -6 –8.7 X 10 -5 M is determined fluorimetrically with lumogallion by an extraction—flow injection procedure. Sensitivity and selectivity are improved compared to those obtained earlier with an entirely aqueous system. The transient phenomena occurring in extraction in a narrow teflon tube are examined by a laser excitation technique, in which the concentration profile of the gallium—lumogallion complex is measured directly without phase separation. The results show that mixing between segments plays an important role in dispersion of the sample in the extraction coil. Two extraction processes with different rates were observed in the transient signals during extraction.

Heterotopic salivary gland tissue in lymph nodes of the cervical region

The occurrence and composition of intranodal salivary gland tissue (ISGT) in the head and neck region were investigated. ISGT was mainly composed of mature acini, intercalated ducts and intralobular ducts but immature acini (20.8%) and immature small ducts (18.8%) were also observed. The latter resembled salivary gland tissue in an early developmental stage. Various histological changes of ISGT were observed including cyst formation (16.7%), oncocytic metaplasia (8.3%), oncocytic adenomatous hyperplasia (8.3%) and ductal proliferation (20.8%). Thus ISGT may contain immature components with a potential for differentiation and proliferation.

Immunohistochemical detection of desmosomes in oral squamous cell carcinomas: Correlation with differentiation, mode of invasion, and metastatic potential

An expression of desmosomal glycoprotein 1 (DG 1) was immunohistochemically examined in 77 biopsies and 21 metastatic cervical lymph nodes of oral squamous cell carcinomas (SCC). In the primary tumors the DG 1 expression was significantly reduced at the invasive site of poorly differentiated and highly invasive tumors. In cases of metastases in cervical lymph nodes, the DG 1 staining at the invasive site of the primary tumor was significantly less than that of nonmetastatic cases. The DG 1 expression in the metastatic lymph nodes was as weak as that in the primary tumor. Thus, we suggest that immunohistochemical investigation of DG 1 expression in oral SCC is valuable in predicting tumor behavior.

Time‐resolved diode laser spectroscopy of the ν6 band of propargyl produced by the UV photolysis of allene

The propargyl radical (CH2C≡CH) produced by the ArF excimer laser photolysis of allene was observed by time‐resolved infrared diode laser spectroscopy. More than one hundred and fifty absorption lines have been assigned to the ν6 (CH2‐wagging) fundamental band of propargyl. Most of the absorption lines were observed as doublets due to the spin–rotation interaction in the 2B1 ground electronic state. The rotational and spin–rotation interaction constants derived for the ground vibrational state are, A0=9.608 47(36), B0=0.317 674(24), C0=0.307 098(24), eaa=−0.017 58(95), and ebb=−0.000 355(76) cm−1, where the figures in parentheses are 2.5 times standard deviations to be attached to the last digit. The ν6 band origin is 687.176 03(62) cm−1, consistent with the infrared spectrum observed in the argon matrix. Anomalously large vibrational changes in the A rotational constant and the ΔK centrifugal distortion constant are accounted for by the a‐type Coriolis interaction between the ν6 and ν10 states, where ν10...

Mode of tumor invasion in oral squamous cell carcinoma: Improved grading based on immunohistochemical examination of extracellular matrices

To predict the nodal involvement of oral squamous cell carcinoma (OSCC) many investigators have studied the histologic features of primary tumors. However, conventional histologic grading still is not sufficient to provide an objective and practical evaluation. In this study we tried to modify one standard type of histologic grading, namely, mode of invasion (MI), on the basis of the extracellular matrix (ECM) staining pattern.

A widespread intramucosal naevus associated with hypertrophy of oral mucosa and alveolar bone: a case report

A case of widespread intramucosal naevus with unusual clinical expression associated with hypertrophy of the oral mucosa and maxilla is presented. Hypertrophy was seen in the alveolar bone and all layers of the oral mucosa just beneath the pigmented area. The diagnosis and further examination is discussed.

Clinical and immunohistological study of squamous cell carcinoma of the lower gum. Mode of invasion and distribution of extracellular matrix.

腫瘍の下顎骨への浸潤状態と組織学的腫瘍浸潤様式ならびに腫瘍における細胞外基質出現状態との関連性について下顎歯肉扁平上皮癌37症例で臨床的, 免疫組織学的に検索した。腫瘍の下顎骨への浸潤状態については, レントゲン検査による下顎骨の吸収様式と吸収程度について検索した。下顎骨の吸収様式と吸収程度は Swearingen と戸塚の分類を用いた。組織学的腫瘍浸潤様式は山本, 小浜の分類を用いた。さらに, MIIIを細胞外基質出現状態によってMIIIaとMIIIbに細分類した。細胞外基質についてはラミニン, タイプIVコラーゲン, ヘパラン硫酸プロテオグリカン, ファイブロネクチン, テネイシン, ビトロネクチン, デコリンについて免疫組織学的に検索した。結果は以下のごとくであった。1) Tの程度と腫瘍の下顎骨への浸潤状態の間に明らかな関連性が認められた。2) 組織学的腫瘍浸潤様式と腫瘍の下顎骨への浸潤状態の間に関連性が認められた。3) レントゲン検査による下顎骨の吸収様式と細胞外基質出現状態との間に関連性が認められた。これらの結果より, これら因子の詳細な解析によって下顎歯肉扁平上皮癌の治療計画を決めるための有用な情報が与えられることが示唆された。