Takeshi Matsumori

Studies on liver cirrhosis—effect of elastase on development and recovery of experimental liver fibrosis

Previous experiment showed that serum monoamine oxidase (MAO) level was moderately elevated in hepatic fibrosis and in serious hepato-cellular necrosis. Present study describes the partial purification of MAO in serum, liver mitochondria and various connective tissues such as granuloma, bone and lung by starch block electrophoresis and by ammonium sulfate fractionation. MAO from liver mitochondria has a different properties in heat sensitiveness and in lathyrogen inhibition from that of serum and various connective tissues. Three types of seurm MAO are determined electrophoretically. One of them are markedly elevated in the serum of liver cirrhosis. MAO fi-om liver mitochondria moves towards anode with two peaks. On the other hand MAO from various connective tissues moves towards cathod with two peaks. MAO in the saturation from twenty to thirty-five percent of ammonium sulfate markedly elevated in the serum of liver cirrhosis. Serum MAO in hepatoceUular necrosis can not be completely inhibited by 0 . I / t mole of aminoacetonitrile in vitro and has a similar electrophoretic pattern like that of liver mitochondria On the contrary, serum MAO in hepatic fibrosis has a different electrophoretic pattern from that of liver mitochondria and completely inhibited by lathyrogens. These results suggest that (i) elevation of serum MAO in hepatic fibrosis and in serious hepatocellular necrosis may be depend on the elevation of different component of isozyme. (ii) Serum MAO in hepatic fibrosis may be responsible with that of connective tissue. (iii) Serum MAO in hepatocellular necrosis may be originated from that of liver mitochondria.

Studies on carbohydrate metabolism in experimemtal fatty liver (III) With special reference to metabolism of glucose or xylitol

ConclusionIn choline-deficient fatty liver, oxidation of xylitol or glucose and its participation in glycogenesis dropped in parallel with development of fatty liver, while the mechanism of emitting liver TG was rather in promoted state at the initial stage, and decreased thereafter, which suggests that the administration of xylitol or glucose may favour glycogenesis and maintenance of energy metabolism and, moreover, it may play some role in the maintenance of mechanism of emitting liver TG.