Taketo Shimoyama

The Promoting Effects of Food Dyes, Erythrosine (Red 3) and Rose Bengal B (Red 105), on Thyroid Tumors in Partially Thyroidectomized N‐Bis(2‐hydroxypropyl)‐ nitrosamine‐treated Rats

The effects of erythrosine (Red 3), rose bengal B (Red 105) and thyroidectomy on the development of thyroid tumor were examined in male Wistar rats treated with N‐bis(2‐hydroxypropyl)nitrosamine (DHPN). Red 3 and Red 105 were used at 4% in the basal diet and were administered for 19 weeks from week 2 to 20. Thyroidectomy was performed by resection of the left lobe at week 4. Single injection of DHPN was performed intraperitoneally at 280 mg per 100 g body weight at the beginning of the experiment. Red 3 and Red 105 significantly promoted the development of thyroid tumors in thyroidectomized rats given DHPN, but had no significant effect in non‐thyroidectomized rats. The incidence of thyroid tumors was 91% in rats with partial thyroidectomy, Red 3 and DHPN, 100% in rats with partial thyroidectomy, Red 105 and DHPN, and 64% in rats with partial thyroidectomy and DHPN. Serum TSH was 5.5±3.1 ng/ml in rats with partial thyroidectomy, Red 3 and DHPN, 2.1 ± 2.2 ng/ml in rats with partial thyroidectomy, Red 105 and DHPN, and 1.5±0.5 ng/ml in rats with partial thyroidectomy and DHPN.

MALIGNANT “RITON” TUMOR WITH METASTATIC HEMANGIOPERICYTOMA IN A PATIENT ASSOCIATED WITH VON RECKLINGHAUSEN'S DISEASE

An autopsy case of malignant schwannoma in a 31‐year‐old female associated with von Recklinghausens disease with unusual elements is reported. The patient who was diagnosed as von Recklinghausens disease from a biopsy specimen of a subcutaneous tumor in her shoulder and multiple café au lait spots in her chest and back, complained of gradual increase in pain in her lower abdomen. A large mass measuring about 18 cm in diameter was located in her left retroperitoneum. Malignant schwannoma associated with von Recklinghausens disease was indicated by the cellularity of the tumor, intersecting bundles of spindle cells, alternating cellular and less cellular myxoid areas in the peripheral portion of the tumor, whereas a large part of the tumor had a hemangiopericytoma‐like arrangement with gaping blood vessels. In addition, however, foci of rhabdomyosarcomatous differentiation and of angiosarcomatous components were found in some areas of this tumor. Moreover, intravascular schwannian growth, which was presumably related to von Recklinghausens disease, was observed in the heart and thyroid. This tumor should be classified as a malignant triton tumor because of the presence of rhabdomyoblasts.

Carcinogenicity study of a commercial sodium oleate in fischer rats

Sodium oleate was added to the drinking-water of groups of 50 male and 50 female F344 rats at levels of 2.5 and 5.0% for 108 wk. The mean liver weight in males given 5% oleate was lower than that of the males given 2.5% oleate or distilled water alone. The mean thymus weight of females given 5% oleate was higher than that of females given 2.5% oleate or distilled water. There were no statistically significant differences between treated and control rats in the results of the urine and serum analyses or haematological determinations or in the incidence of tumours, apart from pancreatic tumours. The latter, in the males, showed some increase over the concurrent controls but did not differ significantly from the reported spontaneous incidence in this strain. It was concluded that sodium oleate does not induce tumours when given orally to F344 rats.

Primary Sjögren's Syndrome Followed by Chronic Myelogenous Leukemia: A Case Report with a Ten Year History

We describe a female patient with primary Sjögrens syndrome who subsequently developed chronic myelogenous leukemia. The patient received a bone marrow transplant (BMT) from a genotypically HLA‐identical brother after pre‐transplant preparation with busulfan and cyclophosphamide. Eighteen weeks later, cutaneous and mucosal chronic graft‐versus‐host disease developed, followed by death 11 months after transplantation. Anti‐Ro/SS‐A antibodies disappeared within 6 months post‐BMT, but antinuclear antibodies remained positive through the post‐transplant course.

Effects of trisodium nitrilotriacetate monohydrate, nitrilotriacetic acid and ammonium chloride on urinary bladder carcinogenesis in rats pretreated with N-bis(2-hydroxypropyl) nitrosamine

The effects of trisodium nitrilotriacetate monohydrate (Na3 NTA.H2O) nitrilotriacetic acid (H3NTA) and ammonium chloride (NH4Cl) on two-stage urinary bladder carcinogenesis were examined. Carcinogenesis was initiated by administration of 0.2% N-bis (2-hydroxypropyl)-nitrosamine (DHPN) to male Wistar rats in the drinking water for 2 weeks, and then the animals were treated with basal diet containing Na3NTA.H2O, Na3NTA.H2O plus NH4Cl, H3NTA, H3NTA plus NH4Cl, or without these chemicals for 28 weeks. Na3NTA.H2O increased significantly the resultant incidence of neoplastic and preneoplastic lesions of the urinary bladder. Moreover, treatment with Na3NTA.H2O, without the initiation, itself induced papillary or nodular (PN)-hyperplasia. H3NTA produced only a slight increase in the incidence of preneoplastic urinary bladder lesions (PN-hyperplasia) in rats initiated by DHPN, and this was not statistically significant. Elevation of both pH and sodium ion concentration in the urine were correlated with promotion of tumor development. These data showed that Na3NTA.H2O was more effective than H3NTA with regard to promoting potential, and that changes in both urinary pH and concentration of sodium played important roles in enhancement of urinary bladder tumorigenesis by these chemicals.

Primary systemic amyloidosis with severe cardiac amyloidosis.

An autopsy case of primary systemic amyloidosis with severe cardiac amyloidosis in a 75‐year‐old Japanese man is reported. The initial signs were dyspnea and cough, followed by general fatigue and loss of appetite. Heart failure, pleural effusion, and liver cysts were found on physiological examination. Autopsy findings were primary sytemic amyloidosis with severe diffuse cardiac amyloidosis and cardiac hypertrophy (585 g). Focal necrosis by infarction was found in the liver and spleen. The cause of amyloidosis was unknown. ACTA PATHOL. JPN. 35: 905–914, 1985.