Talitha Santini

Microbially-driven strategies for bioremediation of bauxite residue

Globally, 3 Gt of bauxite residue is currently in storage, with an additional 120 Mt generated every year. Bauxite residue is an alkaline, saline, sodic, massive, and fine grained material with little organic carbon or plant nutrients. To date, remediation of bauxite residue has focused on the use of chemical and physical amendments to address high pH, high salinity, and poor drainage and aeration. No studies to date have evaluated the potential for microbial communities to contribute to remediation as part of a combined approach integrating chemical, physical, and biological amendments. This review considers natural alkaline, saline environments that present similar challenges for microbial survival and evaluates candidate microorganisms that are both adapted for survival in these environments and have the capacity to carry out beneficial metabolisms in bauxite residue. Fermentation, sulfur oxidation, and extracellular polymeric substance production emerge as promising pathways for bioremediation whether employed individually or in combination. A combination of bioaugmentation (addition of inocula from other alkaline, saline environments) and biostimulation (addition of nutrients to promote microbial growth and activity) of the native community in bauxite residue is recommended as the approach most likely to be successful in promoting bioremediation of bauxite residue.

Spontaneous vegetation encroachment upon bauxite residue (red mud) as an indicator and facilitator of in situ remediation processes.

The spontaneous colonization of a bauxite residue (alumina refining tailings) deposit by local vegetation in Linden, Guyana, over 30 years, indicates that natural weathering processes can ameliorate tailings to the extent that it can support vegetation. Samples were collected from vegetated and unvegetated areas to investigate the relationships between bauxite residue properties and vegetation cover. Compared to unvegetated areas, bauxite residue in vegetated areas had lower pH (mean pH 7.9 vs 10.9), lower alkalinity (mean titratable alkalinity 0.4 vs 1.4 mol H(+) kg(-1)), lower electrical conductivity (mean EC 0.3 vs 2.1 mS cm(-1)), lower total Al (mean Al2O3 19.8 vs 25.8% wt) and Na (mean Na2O 0.9 vs 3.7% wt), and less sodalite and calcite. Accumulation of N, NH4(+), and organic C occurred under vegetation, demonstrating the capacity for plants to modify residue to suit their requirements as a soil-like growth medium. Aeolian redistribution of coarse grained tailings appeared to support vegetation establishment by providing a thin zone of enhanced drainage at the surface. Natural pedogenic processes may be supplemented by irrigation, enhanced drainage, and incorporation of sand and organic matter at other tailings deposits to accelerate the remediation process and achieve similar results in a shorter time frame.

In situ neutralisation of uncarbonated bauxite residue mud by cross layer leaching with carbonated bauxite residue mud

Unameliorated residue mud from the Bayer process generates highly alkaline leachates (pH ca. 13) after deposition in storage areas. Pre-deposition treatment of bauxite residue mud (BRM) with CO(2) gas (carbonation) lowers leachate pH to ca. 10.5. Laboratory scale leaching columns were used to investigate the potential for in situ pH reduction in existing uncarbonated BRM deposits through exposure to carbonated mud leachate. Leachates from uncarbonated and carbonated residues in single and dual-layer column configurations were analysed for pH, electrical conductivity, carbonate and bicarbonate content, and element concentrations. Air-dried solids were analysed by X-ray diffraction before and after leaching. Cross layer leaching lowers leachate pH from uncarbonated BRM. Leachate pH was significantly lower in dual layer and carbonated residue than in uncarbonated residue between one and 400 pore volumes leached. Carbonated residue porewater as well as dawsonite and calcite dissolution were identified as sources of (bi-)carbonate. Leachate concentrations of As, Cr, Cu, Ga and La were immediately reduced in dual layer treatments compared with uncarbonated residue. No element analysed exhibited a significantly higher leachate concentration in dual layer treatments than the highest observed concentration in single layer treatments. The implementation of dual layer leaching in the field therefore presents an opportunity to improve leachate quality from existing uncarbonated residue deposits and justifies further testing at field scale.

Microbial Diversity in Engineered Haloalkaline Environments Shaped by Shared Geochemical Drivers Observed in Natural Analogues

ABSTRACT Microbial communities in engineered terrestrial haloalkaline environments have been poorly characterized relative to their natural counterparts and are geologically recent in formation, offering opportunities to explore microbial diversity and assembly in dynamic, geochemically comparable contexts. In this study, the microbial community structure and geochemical characteristics of three geographically dispersed bauxite residue environments along a remediation gradient were assessed and subsequently compared with other engineered and natural haloalkaline systems. In bauxite residues, bacterial communities were similar at the phylum level (dominated by Proteobacteria and Firmicutes) to those found in soda lakes, oil sands tailings, and nuclear wastes; however, they differed at lower taxonomic levels, with only 23% of operational taxonomic units (OTUs) shared with other haloalkaline environments. Although being less diverse than natural analogues, bauxite residue harbored substantial novel bacterial taxa, with 90% of OTUs nonmatchable to cultured representative sequences. Fungal communities were dominated by Ascomycota and Basidiomycota, consistent with previous studies of hypersaline environments, and also harbored substantial novel (73% of OTUs) taxa. In bauxite residues, community structure was clearly linked to geochemical and physical environmental parameters, with 84% of variation in bacterial and 73% of variation in fungal community structures explained by environmental parameters. The major driver of bacterial community structure (salinity) was consistent across natural and engineered environments; however, drivers differed for fungal community structure between natural (pH) and engineered (total alkalinity) environments. This study demonstrates that both engineered and natural terrestrial haloalkaline environments host substantial repositories of microbial diversity, which are strongly shaped by geochemical drivers.

The geomicrobiology of mining environments

As the global population increases, so does the demand for minerals and energy resources. Demand for some of the major global commodities is currently growing at rates of: copper - 1.6% p.a.(1); iron ore: 1.4% p.a.(2); aluminium - 5% p.a.(3); rare earth elements - 7% p.a.(4), driven not only by population growth in China, India, and Africa, but also by increasing urbanisation and industrialisation globally. Technological advances in renewable energy production and storage, construction materials, transport, and computing could see demand for some of these resources spike by 2600% over the next 25 years under the most extreme demand scenarios(5). Coupled with declining ore grades, this demand means that the global extent of mining environments is set to increase dramatically. Land disturbance attributed to mining was estimated to be 400 000 km(2) in 2007(6), with projected rates of increase of 10 000 km(2) per year(7). This will increase the worldwide extent of mining environments from around 500 000 km(2) at present to 1 330 000 km(2) by 2100, larger than the combined land area of New South Wales and Victoria (1 050 000 km(2)), making them a globally important habitat for the hardiest of microbial life. The extreme geochemical and physical conditions prevalent in mining environments present great opportunities for discovery of novel microbial species and functions, as well as exciting challenges for microbiologists to apply their understanding to solve complex remediation problems.

Microbial Fermentation of Organic Carbon Substrates Drives Rapid pH Neutralization and Element Removal in Bauxite Residue Leachate

Globally, mineral processing activities produce an estimated 680 GL/yr of alkaline wastewater. Neutralizing pH and removing dissolved elements are the main goals of wastewater treatment prior to discharge. Here, we present the first study to explicitly evaluate the role of microbial communities in driving pH neutralization and element removal in alkaline wastewaters by fermentation of organic carbon, using bauxite residue leachate as a model system, and evaluate the effects of organic carbon complexity and microbial inoculum addition rates on the performance of these treatment systems at laboratory scale. Rates and extents of pH neutralization were higher in bioreactors fed with simpler organic carbon substrates (glucose and banana: 6 days to reach pH ≤ 8) than those fed with more complex organic carbon substrates (eucalyptus mulch: 15 days to reach pH ≤ 8; woodchips: equilibrium pH around 9). Concentrations of dissolved Al, As, B, Mo, Na, S, and V all significantly decreased after bioremediation. Increasing soil inoculant addition rate accelerated rates and extent of pH neutralization and element removal up to 0.1 wt %; further increases had little effect. Overall, glucose added at 1.8 wt % and soil inoculum added at 0.1 wt % provided the most effective minimal combination of carbon substrate and inoculum to drive pH neutralization and element removal.

Late-Holocene cliff-top blowout activation and evolution in the Cooloola Sand Mass, south-east Queensland, Australia

Cliff-top dunes are a locally important geomorphic features of sedimentary coasts. They are traditionally interpreted as being sourced by (or with) sand derived from the beach below the cliff. This paper presents the results of a stratigraphic and geochronological study of Carlo Sand Blow, a coastal blowout that has developed on top of a high sandy cliff in the Cooloola Sand Mass, south-east Queensland. We use a combination of sedimentological, pedological and geophysical techniques along with optically stimulated luminescence dating to determine the depositional history and evolution of the blowout. We demonstrate that the blowout is dominantly nourished by sand eroded from its floor rather than the adjacent beach. The original dune surface dates to the first half of the last glacial period (c. 40–70 ka) and this dune was deflated in the late-Holocene. Dune activity is directly associated with cliff undercutting because of coastal retreat in the late-Holocene, but coastal erosion on its own is not capable of maintaining aeolian activity. Blowout activity occurred between 2.6 and 2.3 ka and again at 0.3 ka with aeolian sand burying palaeosols. Both soil surfaces contained charcoal and tree stumps in growth position and our study suggests that fire is the immediate trigger for blowout reactivation. It is likely that these fires were anthropogenic in origin, because the site is somewhat protected from natural fire and the ages coincide with intensification of human use of coastal sites in the area.