Taneaki Oikawa
Yamagata University
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Featured researches published by Taneaki Oikawa.
Journal of Reproductive Immunology | 1987
Yoshihiko Araki; Shoichiro Kurata; Taneaki Oikawa; Takao Yamashita; Masahiko Hiroi; Masaharu Naiki; Fujiro Sendo
A monoclonal antibody (mAb) designated AZPO-8 was produced by hybridizing a mouse myeloma with spleen cells from BALB/c mice immunized with materials obtained from the hamster oviduct. With an immunofluorescence test, AZPO-8 reacted with the zona pellucida (ZP) of ovulated eggs in the oviduct (ZP-OVI) but not with the zona pellucida of eggs in the ovary (ZP-OVA). Using indirect enzyme immunostaining, this mAb reacted with epithelial cells of the oviduct, the uterus (especially the cervical epithelium) and the gastric mucosa, but not with other hamster tissues examined. The reactivity of antigen-positive tissues was abrogated by pretreatment of the tissues with periodic acid. Western blotting analysis revealed that AZPO-8 reacted with substances of broad molecular weight range, and the strongest reactivity was detected at a molecular weight of approximately 200,000 in both cases when extract of ZP-OVI or the hamster oviduct was applied on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel. AZPO-8 showed strong hemagglutination activity only to group A human red blood cells. These results indicated that (1) ZP-OVI had an antigen that was not detected on ZP-OVA, (2) ZP-OVI and the oviduct shared the same antigenicity, and (3) the antigenic determinant reactive with the mAb might be carbohydrate in nature. A possible role of this antigen in fertilization was discussed.
Development Genes and Evolution | 1992
Hiroyuki Abe; Hiroaki Kinoh; Taneaki Oikawa; Norio Suzuki
SummaryThe immunocytochemical localization of the egg-jelly macromolecule, a fucose sulphate glycoconjugate (FSG) that induces the acrosome reaction in spermatozoa, was investigated in ovaries of the sea urchin Hemicentrotus pulcherrimus by use of a polyclonal antibody. The polyclonal antibody reacted with the accessory cells and oocytes in the ovarian lumen. In the accessory cells, evidence of an intense immunohistochemical reaction was observed in many globules of variable density. Products of the specific immunohistochemical reaction were frequently observed in the surface region of oocytes, at a distance from the ovarian wall. At the ultrastructural level, the polyclonal antibody was found to react with the material present in the vacuole-like structures of the globules in the accessory cells. Many gold particles, demonstrating specific immunolabelling, were associated with well-developed microvilli on the vitellogenic oocytes. In the mature oocytes, intense labelling was observed in the jelly coat but not in the vitelline coat. By contrast, oogonia and early oocytes were barely labelled. Quantitative data indicated that the extent of immunolabellings in the surface region of oocytes was very high in the vitellogenic and mature oocytes. In all cases, neither the oocyte cytoplasm nor the subcellular organelles were labelled. These results suggest that FSG is produced by the accessory cells and is deposited initially on the surface of vitellogenic oocytes for the formation of jelly. These findings may provide a new insight into the role of the accessory cells in the reproductive process of the sea urchin.
Gamete Research | 1988
Taneaki Oikawa; Yutaka Sendai; Shoichiro Kurata; Ryuzo Yanagimachi
Anatomical Record-advances in Integrative Anatomy and Evolutionary Biology | 1993
Hiroyuki Abe; Taneaki Oikawa
Anatomical Record-advances in Integrative Anatomy and Evolutionary Biology | 1993
Hiroyuki Abe; Taneaki Oikawa
Anatomical Record-advances in Integrative Anatomy and Evolutionary Biology | 1992
Hiroyuki Abe; Taneaki Oikawa
Journal of Experimental Zoology | 1990
Hiroyuki Abe; Taneaki Oikawa
Journal of Reproductive Immunology | 1988
Yasuko Sakai; Yoshihiko Araki; Takao Yamashita; Shoichiro Kurata; Taneaki Oikawa; Masahiko Hiroi; Fujiro Sendo
Anatomical Record-advances in Integrative Anatomy and Evolutionary Biology | 1991
Hiroyuki Abe; Taneaki Oikawa
Gamete Research | 1978
Taneaki Oikawa