Tanja Bohinc

Sowing mixtures of Brassica trap crops is recommended to reduce Phyllotreta beetles injury to cabbage

Abstract We studied the extent of damage by cabbage flea beetles on four different Brassica species in a two-year field experiment (2009–2010) at two locations in Slovenia. The entire experiment was based on testing oilseed rape, white mustard and oil radish as potential trap crops to protect cabbage from cabbage flea beetles. A significant influence of the Brassica species on the feeding by the flea beetles was confirmed at both locations. The damage index on oil radish was the highest throughout most of the growth period, whereas oilseed rape and white mustard were preferred only during a certain growth period. The initial damage by the cabbage flea beetles occurred in the first half of May, whereas the greatest damage occurred at the beginning of July. The research shows that the onset of cabbage flea beetle feeding can be controlled in a medium-late cabbage cultivar using trap cropping. However, because none of the tested trap crops strongly attracted the flea beetles throughout the entire growing period of the crop, we recommend sowing mixtures of crops for cabbage production; thus, each of the three Brassica species would attract phytophagous insects during a particular part of the cabbage growing season.

Testing the efficacy of single applications of five insecticides against Scaphoideus titanus on common grapevines

The appearance of the Flavescence Dorée phytoplasma and the possibility of its rapid spread by its vector, the American Grapevine Leafhopper (Scaphoideus titanus Ball) (AGVL), represent a serious threat to viticulture production in Slovenia and elsewhere in Europe. Insecticide treatment represents one of major means of reducing the abundance of AGVL in wine-growing areas. In the period from 2010 to 2011, five insecticides against AGVL were tested, and the efficacy of the substances was calculated according to the number of nymphs (at 2, 7, 14 and 21 d after application), which were counted on the grapevine leaves. In both years, the highest cumulative efficacy (96–97% or 0.01 nymphs/leaf) was confirmed for thiamethoxam, whereas chlorpyrifos-methyl showed a very high cumulative efficacy (87–89% or 0.01–0.02 nymphs/leaf). In 2010, indoxacarb exhibited only a 43% (0.09 nymphs/leaf) cumulative efficacy; however, its substitute in 2011, chlorpyrifos, was more effective (73% or 0.05 nymphs/leaf). A single application of pyrethrin demonstrated only 45–61% (0.05–0.09 nymphs/leaf) efficacy. Based on the results of our research, we recommend the use of thiamethoxam and chlorpyrifos-methyl against AGVL.

Cruciferous plants' use as biofumigants in potato against wireworms

In Slovenia, only tefluthrin is registered for controlling wireworms. In order to investigate the potential alternatives for controlling wireworms in potato, the field experiment was conducted in the period from 2011 (year 1) to 2012 (year 2). As alternatives, different Brassica crops (year 1: rapeseed, oilseed radish and white mustard; year 2: rapeseed, oilseed radish, oilseed rape, kale and white mustard) were tested (fresh foliage was incorporated into the soil by ploughing) for their biofumigant effect. Results of our investigation showed that in both years the potato yield was affected by measures of pest control; however, none of the tested biofumigant plants proved to be a potentially promising candidate in wireworm control. The rapid release of volatiles after plant chopping before and during incorporation into the soil could be the reason of inefficacy in our experiment. In our study, most wireworm damage occurred on biggest potato tubers. Moreover, edge effect of the field played an important role in our experiment in year 1. Treatment (chemical or biological) of wireworms only at the edge part of the field could offer a cost-effective, time-saving and environmentally more friendly way of controlling studied pest organism.

Leaf epicuticular wax as a factor of antixenotic resistance of cabbage to cabbage flea beetles and cabbage stink bugs attack

The aim of present research was to establish the role of epicuticular wax content in eight cabbage genotypes (four white hybrids and one red hybrid, two red varieties and one white variety) in the context of its natural resistance to attack cabbage flea beetles (Phyllotreta spp.) and cabbage stink bugs (Eurydema spp.), which are among the most important cabbage pests in southern Europe. For this reason and for the purpose of diminishing the use of synthetic insecticides against the cabbage pests the field experiments in 2006 and 2008 were conducted. We found out that individual cabbage genotypes – they had different epicuticular wax content – differ in regard to their susceptibility to attacks by the studied groups of harmful insect pests. The highest susceptibility to attacks by Phyllotreta spp. was confirmed for the hybrid ‘Cheers F1’, in the first year (1.68 ± 0.05), as well as in the second year of the experiment (2.87 ± 0.13). Cabbage stink bugs in both years of the experiment caused the highest extent of injuries on the hybrids ‘Destiny F1’, ‘Cheers F1’, and ‘Vestri F1’. In both years we found higher epicuticular wax content in red cabbage genotypes. In almost all studied genotypes we found a pronounced negative correlation between the content of epicuticular wax and the extent of injuries done by both groups of harmful pests. We have established that epicuticular wax is an important factor of cabbages antixenotic resistance to attacks by cabbage flea beetles and cabbage stink bugs, and that the cabbage genotypes with higher content of this substance are consequently more suitable for environmentally acceptable manners of cabbage production.

The effect of a mixture of two plant growth-promoting bacteria from Argentina on the yield of potato, and occurrence of primary potato diseases and pest – short communication

ABSTRACT In 2017, we conducted a field experiment to test the influence of a mixture of two plant growth-promoting bacteria (Pseudomonas fluorescens and Azospirillum brasilense) on the yield of three potato varieties. In addition, the influence of the mixture on potato susceptibility to infection by the pseudofungus Phytophthora infestans and fungus Alternaria solani and to attacks by the Colorado potato beetle (Leptinotarsa decemlineata) was investigated. It was the first experiment of its kind in Europe. In the unusually hot and dry year, a positive influence on the yield (17%–31%) was found in all three varieties when the tubers were soaked in the bacterial mixture prior to sowing. We observed no significant differences in potato resilience/susceptibility to harmful organisms among the different treatments (soaked tubers, sprayed tubers, untreated tubers); however, there were significant differences in the productivity between the tuber varieties. The results of our research indicate the bacterial mixture used in this study has a high potential to support satisfactory potato yields under dry conditions and under low levels of infection by foliar fungal diseases and attacks by foliar insects.

Field testing of different synthetic attractants for mass trapping of common European cockchafer (Melolontha melolontha [L.], Coleoptera, Scarabaeidae) adults

ABSTRACT Between 2012 and 2014, we tested the efficacy of different synthetic attractants for the purpose of massive trapping of common European cockchafer adults (Melolontha melolontha). The research took place in three different locations in Slovenia (Otlica, Črni Vrh nad Idrijo, Cesta nad Ajdovščino) during flight periods of adult beetles. In the period 2013–2014, we used, on the basis of the preliminary test results (2012), the following chemicals: toluquinone, cis–3–hexen–1-ol, ethyl acetate, toluquinone + cis–3 hexen–1-ol, and ethanol as a control. M. melolontha adults were most abundant in the location Črni Vrh nad Idrijo; the traps in this location caught 18 times more of them than those in the first location and more than six times more than those in the third location. Male insects accounted for 73 to 87% of the trapped specimens. We confirmed the highest efficiency of cis–3–hexen–1-ol in 2013 (43.25 ± 0.08 males/trap), as well as in 2014 (15.00 ± 0.14 males/trap). On the basis of the trapped adult common European cockchafers and the simple economic analysis of applying different synthetic substances for attracting them, we found that independent application of cis-3-hexen-1-ol is the most efficient and cost-effective option for attracting the studied insect pest. Consequently, we recommend it as an attractant in the traps for massive trapping of adult common European cockchafers.

About Previous Investigations Regarding the Role of Glucosinolates in Controlling Brassica Insect Pests in Slovenia

The chapter presents previous field and laboratory investigations of cabbage flea beetles (Phyllotreta spp.) and cabbage stink bugs (Eurydema spp.) interactions with different Bras‐ sica crops in Slovenia. The special emphasis is given to an influence of different glucosi‐ nolates on injuries caused by mentioned economically important two genera of insect pests. In the study, we found out that the content of glucosinolates differs between differ‐ ent Brassica species, as well as between individual plant organs of the same species. The content of glucosinolates is conditioned also by environmental influences. Among the an‐ alysed glucosinolates, glucobrassicin was presented in all plant species. In almost all plant species, it inhibited the feeding of cabbage flea beetles, except in oil rape, where it had stimulative effects. We have established that the influence of individual glucosinolate on Phyllotreta spp. and Eurydema spp. is not identical as it differs between individual plant species. Because of the variability of glucosinolates as well as different preferences of the studied groups of harmful pests in regard to the plant species, one of the options for diminishing the damage caused by cabbage stink bugs and cabbage flea beetles is the use of mixed Brassica crops for trapping the pests in the growing season. In the future, glucosinolates should be employed to a greater extent in environmentally acceptable ways of food production, one of which is also the use of trap crops in order to reduce harmful effects of cabbage stink bugs.