Tanya D. Ivanova

The origins of neuromuscular fatigue post-stroke.

Fatigue post-stroke is a disabling and persistent symptom affecting many stroke survivors. Despite its high prevalence, the pathophysiology underlying this phenomenon remains obscure. The aim of the present study was to investigate the origins of neuromuscular fatigue post-stroke. Ten chronic stroke survivors and 10 controls sustained an isometric contraction at 30% of maximal voluntary contraction (MVC) with the ankle dorsiflexors. Motor evoked potential (MEP), cortical silent period (SP), voluntary activation, M wave and contractile properties were evaluated before, during and after fatigue among the paretic, non-paretic and control limbs. The pattern of response to fatigue in the non-paretic and control limbs was comparable; therefore, results are presented between the paretic and non-paretic limbs. Before fatigue, reduced MVC peak torque and MEP amplitude were observed on the paretic side in comparison with the non-paretic side. During fatigue, the cortical SP duration increased significantly in both limbs, whereas the MEP amplitude significantly increased only in the non-paretic limb. After fatigue, MVC peak torque decreased significantly in both limbs. Significant reductions in M wave and twitch peak torque were observed in both limbs, pointing to the development of peripheral fatigue. However, central fatigue, evident by a significant reduction in voluntary activation, was greater in the paretic than in the non-paretic limb. After stroke, an inability to increase central excitability in response to an increased cortical inhibition associated with the fatiguing contraction may contribute to central fatigue observed in the paretic limb, which may also be linked to increased self-reported fatigue during activities of daily living. These findings advance our understanding of the neuromuscular basis of fatigue post-stroke.

Reliability of center of pressure measures within and between sessions in individuals post-stroke and healthy controls

BACKGROUNDnKnowing the reliability of the center of pressure (COP) is important for interpreting balance deficits post-stroke, especially when the balance deficits can necessitate the use of short duration trials. The novel aspect of this reliability study was to examine the center of pressure measures using two adjacent force platforms between and within sessions in stroke and controls. After stroke, it is important to understand the contribution of the paretic and non-paretic leg to the motor control of standing balance. Because there is a considerable body of knowledge on COP reliability on a single platform, we chose to examine reliability using two adjacent platforms which has not been examined previously in stroke.nnnMETHODSnTwenty participants post-stroke and 22 controls performed an arm raise, load drop and quiet stance balance task while standing on two adjacent force platforms, on two separate days. Intraclass correlations coefficient (ICC2,1) and percentage standard error of measurement (SEM%) were calculated for COP velocity, ellipse area, anterior-posterior (AP) displacement, and medial-lateral (ML) displacement.nnnRESULTSnBetween sessions, COP velocity was the most reliable with high ICCs and low SEM% across groups and tasks and ellipse area was less reliable with low ICCs across groups and tasks. COP measures were less reliable during the arm raise than load drop post-stroke. Within session reliability was high for COP velocity and ML displacement requiring no more than six trials across tasks.nnnCONCLUSIONSnThe COP velocity was the most reliable measure with high ICCs between sessions and the high reliability was achieved with fewer trials in both groups in a single session.

Retraining Postural Responses With Exercises Emphasizing Speed Poststroke

Background Postural responses are impaired after stroke, with reduced or delayed muscle activity in the paretic leg muscles. Objective The efficacy of exercises emphasizing speed of movement in modifying postural responses to perturbations that were not practiced was investigated. Design This was a dual cohort design. Methods A convenience sample of 32 individuals with hemiparesis poststroke (mean number of weeks poststroke=11.3, SD=4.1) who were recruited upon discharge from an inpatient rehabilitation hospital and a control group of age- and sex-matched individuals who were healthy (n=32) performed a single session of exercise emphasizing speed of movement. To assess postural responses to internal perturbation, unilateral arm raise and load drop tasks were performed before exercises (pre-exercise), immediately after exercises (post-exercise), and 15 minutes after exercises (retention). The time to burst peak and area of the biceps femoris muscle (BF) electromyographic (EMG) activity in the arm raise task was measured with the arm acceleration and velocity of the center of pressure (COP) excursion. For the load drop task, the anticipatory EMG deactivation area of the BF was calculated. In both tasks, the vertical ground reaction forces were recorded for each leg separately. Results Before exercise, EMG and force platform measures were smaller in the stroke group than in the control group. After exercise, the paretic BF time to burst peak decreased, the paretic BF EMG area increased, and the COP velocity increased in the arm raise task, as did the paretic BF anticipatory EMG deactivation area in the load drop task. The stroke group was weight bearing more symmetrically after exercises. Most changes were retained 15 minutes after the exercises. Limitations The retention period was short, and there was no control group of individuals with stroke. Conclusions The results of this efficacy study demonstrated that fast movement exercises improved postural responses to perturbations that were not practiced.

Effects of Fast Functional Exercise on Muscle Activity After Stroke

Background. In stroke rehabilitation, considerable emphasis is placed on improving muscle strength with less focus on the speed of movement. Muscle power (product of force and velocity) is essential for balance and mobility but velocity of movement is impaired after stroke. Objective. The purpose of this efficacy study is to determine if a single session of fast functional movements can increase muscle activation and the speed of movement in participants with a subacute stroke. Methods. In total, 32 individuals poststroke and 32 age- and sex-matched controls performed a single session of 50 fast squats and steps. Electromyographic (EMG) activity was measured bilaterally in the rectus femoris (RF), biceps femoris (BF), tibialis anterior (TA), and soleus muscles. The average EMG area and the movement speed were calculated over 10 trials. The effect of exercise was determined as the change from the second set (Start) to the last set (End) of 10 trials. Results. The stroke group had significant increases in EMG area of the TA, BF, and RF during the squatting exercise. There was an increase in EMG area of the RF and BF when the paretic leg was stepping. Improvements in EMG area of the soleus and RF when the paretic leg was in stance accompanied increases in EMG area when the nonparetic leg was stepping. There was a trend for improved movement speed for both exercises. Conclusion. A single session of exercises emphasizing speed of movement can be used to improve muscle activation in persons with mild to moderately severe strokes.

Control of fast squatting movements after stroke

OBJECTIVEnLittle is known about how residual motor impairments after stroke affect the motor control of fast movements, particularly those that combine postural control and limb movement. The purpose of this study was to examine the influence of stroke on the motor control of fast squatting movements.nnnMETHODSnSeventeen individuals with hemiparesis and seventeen age- and sex-matched controls performed fast squatting movements. Force platform data, knee acceleration, and electromyographic activity from rectus femoris, biceps femoris, tibialis anterior, soleus, were collected.nnnRESULTSnSubjects after stroke performed the squats asymmetrically, with reduced velocity and acceleration compared to controls. Subjects with low motor recovery depended on the non-paretic leg to compensate for poor paretic muscle activation whereas subjects with high motor recovery activated muscles in the paretic leg in an adaptive manner, making the movement more symmetrical. Difficulty with postural control was evident by reduced coupling of the timing of the knee movement with the center of pressure excursion.nnnCONCLUSIONSnSlow performance of squatting movements was accompanied by altered muscle activation, coupled with impaired postural control.nnnSIGNIFICANCEnFast squatting movements in standing require appropriate muscle activation and postural control, the latter of which can be measured easily with force platform and accelerometer data.

Regionalization of the stretch reflex in the human vastus medialis

Regionalization of the stretch reflex, i.e. the notion that the activation of 1a afferents from a muscle region influences only the activation of motor units in the same region, has been demonstrated previously in animals but not in humans. Mechanical stretches applied to regions of vastus medialis as close as 10 mm apart resulted in recruitment of motor units localized topographically with respect to the location of the mechanical stretch. Stretch reflexes are regionalized in the human vastus medialis. The human spinal cord has the neuromuscular circuitry to preferentially activate motoneurones innervating muscle fibres located in different regions of the vastus medialis.

Between-day reliability of triceps surae responses to standing perturbations in people post-stroke and healthy controls: A high-density surface EMG investigation

The reliability of triceps surae electromyographic responses to standing perturbations in people after stroke and healthy controls is unknown. High-Density surface Electromyography (HDsEMG) is a technique that records electromyographic signals from different locations over a muscle, overcoming limitations of traditional surface EMG such as between-day differences in electrode placement. In this study, HDsEMG was used to measure responses from soleus (SOL, 18 channels) and medial and lateral gastrocnemius (MG and LG, 16 channels each) in 10 people after stroke and 10 controls. Timing and amplitude of the response were estimated for each channel of the grids. Intraclass Correlation Coefficient (ICC) and normalized Standard Error of Measurement (SEM%) were calculated for each channel individually (single-channel configuration) and on the median of each grid (all-channels configuration). Both timing (single-channel: ICC=0.75-0.96, SEM%=5.0-9.1; all-channels: ICC=0.85-0.97; SEM%=3.5-6.2%) and amplitude (single-channel: ICC=0.60-0.91, SEM%=25.1-46.6; ICC=0.73-0.95, SEM%=19.3-42.1) showed good-to-excellent reliability. HDsEMG provides reliable estimates of EMG responses to perturbations both in individuals after stroke and in healthy controls; reliability was marginally better for the all-channels compared to the single-channel configuration.

Behavior of medial gastrocnemius motor units during postural reactions to external perturbations after stroke

OBJECTIVEnThis study investigated the behavior of medial gastrocnemius (GM) motor units (MU) during external perturbations in standing in people with chronic stroke.nnnMETHODSnGM MUs were recorded in standing while anteriorly-directed perturbations were introduced by applying loads of 1% body mass (BM) at the pelvis every 25-40s until 5% BM was maintained. Joint kinematics, surface electromyography (EMG), and force platform measurements were assessed.nnnRESULTSnAlthough external loads caused a forward progression of the anterior-posterior centre of pressure (APCOP), people with stroke decreased APCOP velocity and centre of mass (COM) velocity immediately following the highest perturbations, thereby limiting movement velocity in response to perturbations. MU firing rate did not increase with loading but the GM EMG magnitude increased, reflecting MU recruitment. MU inter spike interval (ISI) during the dynamic response was negatively correlated with COM velocity and hip angular velocity.nnnCONCLUSIONSnThe GM utilized primarily MU recruitment to maintain standing during external perturbations. The lack of MU firing rate modulation occurred with a change in postural central set. However, the relationship of MU firing rate with kinematic variables suggests underlying long-loop responses may be somewhat intact after stroke.nnnSIGNIFICANCEnPeople with stroke demonstrate alterations in postural control strategies which may explain MU behavior with external perturbations.

Motor unit recruitment and firing rate in medial gastrocnemius muscles during external perturbations in standing in humans

There is limited investigation of the interaction between motor unit recruitment and rate coding for modulating force during standing or responding to external perturbations. Fifty-seven motor units were recorded from the medial gastrocnemius muscle with intramuscular electrodes in response to external perturbations in standing. Anteriorly directed perturbations were generated by applying loads in 0.45-kg increments at the pelvis every 25-40 s until 2.25 kg was maintained. Motor unit firing rate was calculated for the initial recruitment load and all subsequent loads during two epochs: 1) dynamic response to perturbation directly following each load drop and 2) maintenance of steady state between perturbations. Joint kinematics and surface electromyography (EMG) from lower extremities and force platform measurements were assessed. Application of the external loads resulted in a significant forward progression of the anterior-posterior center of pressure (AP COP) that was accompanied by modest changes in joint angles (<3°). Surface EMG increased more in medial gastrocnemius than in the other recorded muscles. At initial recruitment, motor unit firing rate immediately after the load drop was significantly lower than during subsequent load drops or during the steady state at the same load. There was a modest increase in motor unit firing rate immediately after the load drop on subsequent load drops associated with regaining balance. There was no effect of maintaining balance with increased load and forward progression of the AP COP on steady-state motor unit firing rate. The medial gastrocnemius utilized primarily motor unit recruitment to achieve the increased levels of activation necessary to maintain standing in the presence of external loads.

Motoneurone afterhyperpolarisation time-course following stroke

OBJECTIVEnOur aim was to investigate any changes in the estimated time-course of the afterhyperpolarisation (AHP) in motoneurones innervating the tibialis anterior following stroke, with a secondary objective to compare the results from two different AHP estimation techniques.nnnMETHODSnMotor units from tibialis anterior on the paretic and non-paretic sides of 15 subjects with chronic stroke were recorded using intramuscular electrodes during voluntary isometric contraction. Participants varied the motor unit firing rate from its lowest rate to approximately 10 Hz. The AHP duration was estimated using the interval death rate (IDR) and transition point methods.nnnRESULTSnThe AHP decay time-constant was significantly different between sides (paretic: 41.7 ± 8.5 ms, non-paretic: 36.2 ± 6.4 ms). Additionally, the paretic AHP time-constant was significantly longer in participants with low motor recovery (45.9 ± 9.1 ms) than with high motor recovery (39.3 ± 10.0 ms) as measured by CMSA score. The AHP estimates from the two techniques were correlated (r=0.78).nnnCONCLUSIONSnThe AHP time-course prolongation on the paretic side of people with chronic stroke is more pronounced in people with low motor recovery.nnnSIGNIFICANCEnChanges in the motoneurone AHP time course post-stroke were related to muscle function and may play a role in the commonly-observed reduction of motor unit discharge rate during voluntary contractions following stroke.